An up-to-date review: differential biosynthesis mechanisms and enrichment methods for health-promoting anthocyanins of citrus fruits during processing and storage

References

• Ahmed, O. M., S. F. Abouzid, N. A. Ahmed, M. Y. Zaky, and H. Liu. 2021. An up-to-date review on citrus flavonoids: Chemistry and benefits in health and diseases. Current Pharmaceutical Design 27 (4):513–30. doi: http://dx.doi.org/10.2174/1381612826666201127122313.
   PubMed Web of Science ®Google Scholar
• Allan, A. C., R. P. Hellens, and W. A. Laing. 2008. MYB transcription factors that colour our fruit. Trends in Plant Science 13 (3):99–102. doi: http://dx.doi.org/10.1016/j.tplants.2007.11.012.
   PubMed Web of Science ®Google Scholar
• Barry, G., M. Caruso, and F. Gmitter. 2020. Commercial scion varieties. In The genus citrus, eds. Talon, M., Caruso, M., and Gmitter, 83–104. Sawston Cambridge, United Kingdom: Woodhead Publishing. ISBN9780128121634.
   Google Scholar
• Bernardi, J., C. Licciardello, M. P. Russo, M. Luisa Chiusano, G. Carletti, G. R. Recupero, and A. Marocco. 2010. Use of a custom array to study differentially expressed genes during blood orange (Citrus sinensis L. Osbeck) ripening. Journal of Plant Physiology 167 (4):301–10. doi: http://dx.doi.org/10.1016/j.jplph.2009.09.009.
   PubMed Web of Science ®Google Scholar
• Briskey, D., G. A. Malfa, and A. Rao. 2022. Effectiveness of “Moro” blood orange Citrus Sinensis Osbeck (Rutaceae) standardized extract on weight loss in overweight but otherwise healthy men and women - A randomized double-blind placebo-controlled study. Nutrients 14 (3):427. doi: 10.3390/nu14030427.
   PubMed Web of Science ®Google Scholar
• Buscemi, S., G. Rosafio, G. Arcoleo, A. Mattina, B. Canino, M. Montana, S. Verga, and G. Rini. 2012. Effects of red orange juice intake on endothelial function and inflammatory markers in adult subjects with increased cardiovascular risk. The American Journal of Clinical Nutrition 95 (5):1089–95. doi: 10.3945/ajcn.111.031088.
   PubMed Web of Science ®Google Scholar
• Butelli, E., A. Garcia-Lor, C. Licciardello, G. Las Casas, L. Hill, G. R. Recupero, M. L. Keremane, C. Ramadugu, R. Krueger, Q. Xu, et al. 2017. Changes in anthocyanin production during domestication of citrus. Plant Physiology 173 (4):2225–42. doi: 10.1104/pp.16.01701.
   PubMed Web of Science ®Google Scholar
• Butelli, E., C. Licciardello, Y. Zhang, J. Liu, S. Mackay, P. Bailey, G. Reforgiato Recupero, and C. Martin. 2012. Retrotransposons control fruit-specific, cold-dependent accumulation of anthocyanins in blood oranges. The Plant Cell 24 (3):1242–55. doi: 10.1105/tpc.111.095232.
   PubMed Web of Science ®Google Scholar
• Cardile, V., A. Graziano, and A. Venditti. 2015. Clinical evaluation of Moro (Citrus Sinensis (L.) Osbeck) orange juice supplementation for the weight management. Natural Product Research 29 (23):2256–60. doi: http://dx.doi.org/10.1080/14786419.2014.1000897.
   PubMed Web of Science ®Google Scholar
• Carmona, L., B. Alquézar, C. Diretto, F. Sevi, T. Malara, M. T. Lafuente, and L. Peña. 2021. Curing and low-temperature combined post-harvest storage enhances anthocyanin biosynthesis in blood oranges. Food Chemistry 342:128334. doi: 10.1016/j.foodchem.2020.128334.
   PubMed Web of Science ®Google Scholar
• Carmona, L., B. Alquézar, V. V. Marques, and L. Peña. 2017. Anthocyanin biosynthesis and accumulation in blood oranges during postharvest storage at different low temperatures. Food Chemistry 237 (15):7–14. doi: http://dx.doi.org/10.1016/j.foodchem.2017.05.076.
   PubMedGoogle Scholar
• Carmona, L., B. Alquézar, S. Tárraga, and L. Peña. 2019. Protein analysis of moro blood orange pulp during storage at low temperatures. Food Chemistry 277 (30):75–83. doi: 10.1016/j.foodchem.2018.10.108.
   PubMedGoogle Scholar
• Caruso, M.,S. Fabroni,R. Emma,G. Ballistreri,M. Amenta,W. Currenti,C. Rinzivillo, andP. Rapisarda. 2021. A new standardized phytoextract from red orange and lemon wastes (red orange and lemon extract) reduces basophil degranulation and activation. Natural Product Research 35 (23):5354–9. 10.1080/14786419.2020.1761355.
   PubMed Web of Science ®Google Scholar
• Catalano, C., A. Ciacciulli, F. Salonia, M. P. Russo, P. Caruso, M. Caruso, G. Russo, G. Distefano, and C. Licciardello.,. 2020. Target-genes reveal species and genotypic specificity of anthocyanin pigmentation in citrus and related genera. Genes 11 (7):807. doi: 10.3390/genes11070807.
   PubMed Web of Science ®Google Scholar
• Cebadera-Miranda, L., L. Domínguez, M. I. Dias, L. Barros, I. C. F. R. Ferreira, M. Igual, N. Martínez-Navarrete, V. Fernández-Ruiz, P. Morales, and M. Cámara.,. 2019. Sanguinello and Tarocco (Citrus sinensis [L.] Osbeck): Bioactive compounds and colour appearance of blood oranges. Food Chemistry 270:395–402. doi: http://dx.doi.org/10.1016/j.foodchem.2018.07.094.
   PubMed Web of Science ®Google Scholar
• Chen, J., F. Liu, B. B. Ismail, W. J. Wang, E. B. Xu, H. B. Pan, X. Q. Ye, D. H. Liu, and H. Cheng. 2022. Effects of ethephon and low-temperature treatments on blood oranges (Citrus sinensis L. Osbeck): Anthocyanin accumulation and volatile profile changes during storage. Food Chemistry 393:133381. doi: http://dx.doi.org/10.1016/j.foodchem.2022.133381.
   PubMed Web of Science ®Google Scholar
• Chen, Z., Y. Zhao, D. Jiang, S. Xiang, and J. Li. 2019. Changes in DNA methylation patterns affect ripening time in Satsuma mandarin fruit. Plant Breeding 138 (6):967–78. doi: http://dx.doi.org/10.1111/pbr.12727.
   Web of Science ®Google Scholar
• Chiechio, S., M. Zammataro, M. Barresi, M. Amenta, G. Ballistreri, S. Fabroni, and P. Rapisarda. 2021. A standardized extract prepared from red orange and lemon wastes blocks high-fat diet-induced hyperglycemia and hyperlipidemia in mice. Molecules 26 (14):4291. doi: 10.3390/molecules26144291.
   PubMed Web of Science ®Google Scholar
• Cotroneo, P. S., M. P. Russo, M. Ciuni, G. R. Recupero, and A. R. L. Piero. 2006. Quantitative real-time reverse transcriptase-PCR profiling of anthocyanin biosynthetic genes during orange fruit ripening. Journal of the American Society for Horticultural Science 131 (4):537–43. doi: 10.21273/JASHS.131.4.537.
   Web of Science ®Google Scholar
• Crifò, T., I. Puglisi, G. Petrone, G. Reforgiato Recupero, and A. R. Lo Piero. 2011. Expression analysis in response to low temperature stress in blood oranges: Implication of the flavonoid biosynthetic pathway. Gene 476 (1–2):1–9. doi: 10.1016/j.gene.2011.02.005.
   PubMed Web of Science ®Google Scholar
• Cultrone, A., P. S. Cotroneo, and G. Reforgiato Recupero. 2010. Cloning and molecular characterization of R2R3-MYB and bHLH-MYC transcription factors from Citrus sinensis. Tree Genetics & Genomes 6 (1):101–12. doi: 10.1007/s11295-009-0232-y.
   Web of Science ®Google Scholar
• Damiano, S., V. Iovane, C. Squillacioti, N. Mirabella, F. Prisco, A. Ariano, M. Amenta, A. Giordano, S. Florio, and R. Ciarcia. 2019. Red orange and lemon extract prevents the renal toxicity induced by ochratoxin A in rats. Journal of Cellular Physiology 235 (6):5386–93. doi: 10.1002/jcp.29425.
   Web of Science ®Google Scholar
• Damiano, S., P. Lombari, E. Salvi, M. Papale, A. Giordano, M. Amenta, G. Ballistreri, S. Fabroni, P. Rapisarda, G. Capasso, et al. 2019. A red orange and lemon by-products extract rich in anthocyanins inhibits the progression of diabetic nephropathy. Journal of Cellular Physiology 234 (12):23268–78. doi: 10.1002/jcp.28893.
   PubMed Web of Science ®Google Scholar
• De Felice, E., D. Giaquinto, S. Damiano, A. Salzano, S. Fabroni, R. Ciarcia, P. Scocco, P. de Girolamo, and L. D’Angelo.,. 2021. Distinct pattern of NPY in gastro–entero–pancreatic system of goat kids fed with a new standardized red orange and lemon extract (RLE). Animals 11 (2):449. doi: 10.3390/ani11020449.
   Web of Science ®Google Scholar
• Degu, A., B. Hatew, A. Nunes-Nesi, L. Shlizerman, N. Zur, E. Katz, A. R. Fernie, E. Blumwald, and A. Sadka. 2011. Inhibition of aconitase in citrus fruit callus results in a metabolic shift towards amino acid biosynthesis. Planta 234 (3):501–13. doi: 10.1007/s00425-011-1411-2.
   PubMed Web of Science ®Google Scholar
• Espley, R. V., C. Brendolise, D. Chagné, S. Kutty-Amma, S. Green, R. Volz, J. Putterill, H. J. Schouten, S. E. Gardiner, R. P. Hellens, et al. 2009. Multiple repeats of a promoter segment cause transcription factor autoregulation in red apples. The Plant Cell 21 (1):168–83. doi: 10.1105/tpc.108.059329.
   PubMed Web of Science ®Google Scholar
• Fabroni, S., M. Amenta, N. Timpanaro, A. Todaro, and P. Rapisarda. 2020. Change in taste-altering non-volatile components of blood and common orange fruit during cold storage. Food Research International (Ottawa, Ontario) 131:108916. doi: 10.1016/j.foodres.2019.108916.
   PubMed Web of Science ®Google Scholar
• Fabroni, S., G. Ballistreri, M. Amenta, and P. Rapisarda. 2016. Anthocyanins in different citrus species: An UHPLC–PDA–ESI/MSn‐assisted qualitative and quantitative investigation. Journal of the Science of Food and Agriculture 96 (14):4797–808. doi: 10.1002/jsfa.7916.
   PubMed Web of Science ®Google Scholar
• Ferrara, M., M. F. Sgarro, A. Maggiolino, S. Damiano, F. Iannaccone, G. Mulè, and P. D. Palo. 2021. Effect of red orange and lemon extract-enriched diet in suckling lambs’ fecal microbiota. Agriculture 11 (7):572. doi: 10.3390/agriculture11070572.
   Google Scholar
• Ferré-D'Amaré, A. R., G. C. Prendergast, E. B. Ziff, and S. K. Burley. 1993. Recognition by max of its cognate DNA through a dimeric b/HLH/Z domain. Nature 363 (6424):38–45. doi: 10.1038/363038a0.
   PubMed Web of Science ®Google Scholar
• Ferrer, J. L., J. M. Jez, M. E. Bowman, R. A. Dixon, and J. P. Noel. 1999. Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis. Nature Structural Biology 6 (8):775–84. doi: 10.1038/11553.
   PubMedGoogle Scholar
• Gao, H., Z. K. Zhang, X. G. Lv, N. Cheng, B. Z. Peng, and W. Cao. 2016. Effect of 24-epibrassinolide on chilling injury of peach fruit in relation to phenolic and proline metabolisms. Postharvest Biology and Technology 111:390–7. doi: 10.1016/j.postharvbio.2015.07.031.
   Web of Science ®Google Scholar
• Grosso, G., F. Galvano, A. Mistretta, S. Marventano, F. Nolfo, G. Calabrese, S. Buscemi, F. Drago, U. Veronesi, and A. Scuderi. 2013. Red orange: Experimental models and epidemiological evidence of its benefits on human health. Oxidative Medicine and Cellular Longevity 2013:157240–11. doi: 10.1155/2013/157240.
   PubMed Web of Science ®Google Scholar
• Habibi, F, and A. Ramezanian. 2017. Vacuum infiltration of putrescine enhances bioactive compounds and maintains quality of blood orange during cold storage. Food Chemistry 227:1–8. doi: 10.1016/j.foodchem.2017.01.057.
   PubMed Web of Science ®Google Scholar
• Habibi, F., A. Ramezanian, F. Guillén, S. Castillo, M. Serrano, and D. Valero. 2020. Changes in bioactive compounds, antioxidant activity, and nutritional quality of blood orange cultivars at different storage temperatures. Antioxidants 9 (10):1016. doi: 10.3390/antiox9101016.
   Web of Science ®Google Scholar
• Habibi, F., A. Ramezanian, F. Guillén, D. Martínez-Romero, M. Serrano, and D. Valero. 2020. Susceptibility of blood orange cultivars to chilling injury based on antioxidant system, physiological and biochemical responses at different storage temperatures. Foods 9 (11):1609. doi: http://dx.doi.org/10.3390/foods9111609.
   PubMed Web of Science ®Google Scholar
• Habibi, F., A. Ramezanian, F. Guillén, M. Serrano, and D. Valero. 2020. Blood oranges maintain bioactive compounds and nutritional quality by postharvest treatments with γ-aminobutyric acid, methyl jasmonate or methyl salicylate during cold storage. Food Chemistry 306:125634. doi: 10.1016/j.foodchem.2019.125634.
   PubMed Web of Science ®Google Scholar
• Habibi, F., M. Serrano, L. Zacarías, D. Valero, and F. Guillén. 2021. Postharvest application of 24-epibrassinolide reduces chilling injury symptoms and enhances bioactive compounds content and antioxidant activity of blood orange fruit. Frontiers in Plant Science 12:629733. doi: 10.3389/fpls.2021.629733.
   PubMed Web of Science ®Google Scholar
• Habibi, F., D. Valero, M. Serrano, and F. Guillén. 2022. Exogenous application of glycine betaine maintains bioactive compounds, antioxidant activity, and physicochemical attributes of blood orange fruit during prolonged cold storage. Frontiers in Nutrition 9:873915. doi: 10.3389/fnut.2022.873915.
   PubMed Web of Science ®Google Scholar
• Hamedani, M., V. Rabiei, H. Moradi, A. Ghanbari, and M. R. Azimi. 2012. Determination of storage duration and temperature effects on fruit quality parameters of blood orange (Citrus sinensis cv. Tarocco). Biharean Biologist 6 (1):10–3. doi: 10.13140/RG.2.1.4443.1524.
   Google Scholar
• Hillebrand, S., M. Schwarz, and P. Winterhalter. 2004. Characterization of Anthocyanins and Pyranoanthocyanins from Blood Orange [Citrus sinensis (L.) Osbeck] Juice. Journal of Agricultural and Food Chemistry 52 (24):7331–8. doi: 10.1021/jf0487957.
   PubMed Web of Science ®Google Scholar
• Holton, T. A, and E. C. Cornish. 1995. Genetics and biochemistry of anthocyanin biosynthesis. The Plant Cell 7 (7):1071–83. doi: 10.1105/tpc.7.7.1071.
   PubMed Web of Science ®Google Scholar
• Huang, H., R. Liu, Q. Niu, K. Tang, B. Zhang, H. Zhang, K. Chen, J. K. Zhu, and Z. Lang. 2019. Global increase in DNA methylation during orange fruit development and ripening. Proceedings of the National Academy of Sciences of the United States of America 116 (4):1430–6. doi: 10.1073/pnas.1815441116.
   PubMedGoogle Scholar
• Huang, D., Z. Tang, J. Fu, Y. Yuan, X. Deng, and Q. Xu. 2020. CsMYB3 and CsRuby1 form an ‘activator-and-repressor’ loop for the regulation of anthocyanin biosynthesis in citrus. Plant & Cell Physiology 61 (2):318–30. doi: 10.1093/pcp/pcz198.
   PubMedGoogle Scholar
• Huang, D., X. Wang, Z. Tang, Y. Yuan, Y. Xu, J. He, X. Jiang, S.-A. Peng, L. Li, E. Butelli, et al. 2018. Subfunctionalization of the Ruby2-Ruby1 gene cluster during the domestication of citrus. Nature Plants 4 (11):930–41. doi: 10.1038/s41477-018-0287-6.
   PubMed Web of Science ®Google Scholar
• Huang, D., Y. Yuan, Z. Tang, Y. Huang, C. Kang, X. Deng, and Q. Xu. 2019. Retrotransposon promoter of Ruby1 controls both light- and cold-induced accumulation of anthocyanins in blood orange. Plant, Cell & Environment 42 (11):3092–104. doi: 10.1111/pce.13609.
   PubMed Web of Science ®Google Scholar
• Jaakola, L. 2013. New insights into the regulation of anthocyanin biosynthesis in fruits. Trends in Plant Science 18 (9):477–83. doi: 10.1016/j.tplants.2013.06.003.
   PubMed Web of Science ®Google Scholar
• Kokalj, D., E. Zlati, B. Cigi, and R. Vidrih. 2019. Postharvest light-emitting diode irradiation of sweet cherries (Prunus avium L.) promotes accumulation of anthocyanins. Postharvest Biology and Technology 148:192–9. doi: 10.1016/j.postharvbio.2018.11.011.
   Web of Science ®Google Scholar
• Li, S. 2014. Transcriptional control of flavonoid biosynthesis. Plant Signaling & Behavior 9 (1):e27522. doi: 10.4161/psb.27522.
   PubMed Web of Science ®Google Scholar
• Licciardello, C., N. D'Agostino, A. Traini, G. R. Recupero, L. Frusciante, and M. L. Chiusano. 2014. Characterization of the glutathione S-transferase gene family through ESTs and expression analyses within common and pigmented cultivars of Citrus sinensis (L.) Osbeck. BMC Plant Biology 14 (1):39. doi: 10.1186/1471-2229-14-39.
   PubMedGoogle Scholar
• Licciardello, C., M. P. Russo, G. Vale, and G. Reforgiato Recupero. 2008. Identification of differentially expressed genes in the flesh of blood and common oranges. Tree Genetics & Genomes 4 (2):315–31. doi: 10.1007/s11295-007-0111-3.
   Google Scholar
• Li, Z. T., F. G. Gmitter, Jr., J. W. Grosser, C. Chen, and D. J. Gray. 2012. Isolation and characterization of a novel anthocyanin-promoting MYBA gene family in citrus. Tree Genetics & Genomes 8 (4):675–85. doi: 10.1007/s11295-012-0517-4.
   Web of Science ®Google Scholar
• Li, L., G. K. Lyall, J. A. Martinez-Blazquez, F. Vallejo, F. A Tomas-Barberan, K. M. Birch, and C. Boesch. 2020. Blood orange juice consumption increases flow-mediated dilation in adults with overweight and obesity: A randomized controlled trial. The Journal of Nutrition 150 (9):2287–94.,. doi: 10.1093/jn/nxaa158.
   PubMed Web of Science ®Google Scholar
• Lima, L, and A. Barbosa. 2021. A review of the lipolytic effects and the reduction of abdominal fat from bioactive compounds and moro orange extracts. Heliyon 7:e07695. doi: 10.1016/j.heliyon.2021.e07695.
   PubMedGoogle Scholar
• Lin-Wang, K., K. Bolitho, K. Grafton, A. Kortstee, S. Karunairetnam, T. K. McGhie, R. V. Espley, R. P. Hellens, and A. C. Allan.,. 2010. An R2R3 MYB transcription factor associated with regulation of the anthocyanin biosynthetic pathway in Rosaceae. BMC Plant Biology 10 (1):50. doi: 10.1186/1471-2229-10-50.
   PubMedGoogle Scholar
• Liu, Y., Tikunov, Y. Schouten, R. E. Marcelis, L. F. M. Visser, R. G. F, and Bovy, A. 2018. Anthocyanin biosynthesis and degradation mechanisms in solanaceous vegetables: A review. Frontiers in Chemistry 6:52. doi: 10.3389/fchem.2018.00052.
   PubMed Web of Science ®Google Scholar
• Liu, C., X. Wang, Y. Xu, X. Deng, and Q. Xu. 2014. Genome-wide analysis of the R2R3-MYB transcription factor gene family in sweet orange (Citrus sinensis). Molecular Biology Reports 41 (10):6769–85. doi: 10.1007/s11033-014-3563-1.
   PubMedGoogle Scholar
• Li, D., P. Wang, Y. Luo, M. Zhao, and F. Chen. 2017. Health benefits of anthocyanins and molecular mechanisms: Update from recent decade. Critical Reviews in Food Science and Nutrition 57 (8):1729–41. doi: 10.1080/10408398.2015.1030064.
   PubMed Web of Science ®Google Scholar
• Lo Piero, A. R. 2015. The state of the art in biosynthesis of anthocyanins and its regulation in pigmented sweet oranges [(Citrus sinensis) L. Osbeck]. Journal of Agricultural and Food Chemistry 63 (16):4031–41. doi: 10.1021/acs.jafc.5b01123.
   PubMed Web of Science ®Google Scholar
• Lo Piero, A. R., L. Lo Cicero, and I. Puglisi. 2014. The metabolic fate of citric acid as affected by cold storage in blood oranges. Journal of Plant Biochemistry and Biotechnology 23 (2):161–6. doi: 10.1007/s13562-013-0197-7.
   Web of Science ®Google Scholar
• Lo Piero, A. R., I. Puglisi, and G. Petrone. 2006a. Gene isolation, analysis of expression, and in vitro synthesis of glutathione S-transferase from orange fruit [Citrus sinensis L. (Osbeck)]. Journal of Agricultural and Food Chemistry 54 (24):9227–33. doi: 10.1021/jf0616816.
   PubMed Web of Science ®Google Scholar
• Lo Piero, A. R., I. Puglisi, and G. Petrone. 2006b. Gene characterization, analysis of expression and in vitro synthesis of dihydroflavonol 4-reductase from Citrus Sinensis (L.) Osbeck. Phytochemistry 67 (7):684–95. doi: 10.1016/j.phytochem.2006.01.025.
   PubMed Web of Science ®Google Scholar
• Lo Piero, A. R., I. Puglisi, P. Rapisarda, and G. Petrone. 2005. Anthocyanins accumulation and related gene expression in red orange fruit induced by low temperature storage. Journal of Agricultural and Food Chemistry 53 (23):9083–8. doi: 10.1021/jf051609s.
   PubMed Web of Science ®Google Scholar
• Lu, X., W. Zhou, and F. Gao. 2009. Cloning, characterization and localization of CHS gene from blood orange, Citrus Sinensis (L.) osbeck cv. Ruby. Molecular Biology Reports 36 (7):1983–90. doi: 10.1007/s11033-008-9408-z.
   PubMed Web of Science ®Google Scholar
• Lu, X., W. Zhou, and F. Gao. 2010. Chromosomal location of 45S rDNA and DFR gene in citrus sinensis. Biologia Plantarum 54 (4):798–800. doi: 10.1007/s10535-010-0146-4.
   Web of Science ®Google Scholar
• Magalhães, M. L., R. V. de Sousa, J. R. Miranda, I. F. M. Konig, F. Wouters, F. R. Souza, S. D. Simão, A. da Silva Lunguinho, D. L. Nelson, and M. das Graças Cardoso. 2021. Effects of Moro orange juice (Citrus sinensis (l.) Osbeck) on some metabolic and morphological parameters in obese and diabetic rats. Journal of the Science of Food and Agriculture 101 (3):1053–64. doi: 10.1002/jsfa.10714.
   PubMedGoogle Scholar
• Maghoumi, M., Y. Mostofi, Z. Zamani, A. Talaie, M. Boojar, and P. A. Gomez. 2014. Influence of hot-air treatment, superatmospheric O2 and elevated CO2 on bioactive compounds and storage properties of fresh-cut pomegranate arils. International Journal of Food Science & Technology 49 (1):153–9. doi: 10.1111/ijfs.12290.
   Google Scholar
• Mazza, G, and F. J. Francis. 1995. Anthocyanins in grapes and grape products. Critical Reviews in Food Science and Nutrition 35 (4):341–71. doi: 10.1080/10408399509527704.
   PubMed Web of Science ®Google Scholar
• Mohamad, F., M. Amiri, M. E, and Kahneh, E. 2021. Effect of tea seed oil on post-harvest quality of Moro blood orange. Journal of Horticulture and Postharvest Research 4 (1):115–26. doi: 10.22077/jhpr.2020.3355.1143.
   Google Scholar
• Mohammad, S. S., R. O. Santos, M. I. Barbosa, and J. L. B. Junior. 2021. Anthocyanins: Chemical properties and health benefits: A review. Current Nutrition & Food Science 17 (7):662–72. doi: 10.2174/1573401317999210101150652.
   Web of Science ®Google Scholar
• Molinu, M. G., A. Dore, A. Palma, S. D’Aquino, E. Azara, V. Rodov, and G. D’hallewin. 2016. Effect of superatmospheric oxygen storage on the content of phytonutrients in ‘Sanguinello Comune’ blood orange. Postharvest Biology and Technology 112:24–30. doi: http://dx.doi.org/10.1016/j.postharvbio.2015.09.037.
   Web of Science ®Google Scholar
• Mondello, L., A. Cotroneo, G. Errante, G. Dugo, and P. Dugo. 2000. Determination of anthocyanins in blood orange juices by HPLC analysis. Journal of Pharmaceutical and Biomedical Analysis 23 (1):191–5. doi: 10.1016/S0731-7085(00)00269-7.
   PubMed Web of Science ®Google Scholar
• Montalbano, G., M. Mania, M. C. Guerrera, R. Laurà, F. Abbate, M. Levanti, A. Maugeri, A. Germanà, and M. Navarra. 2019. Effects of a flavonoid-rich extract from Citrus sinensis juice on a diet-induced obese zebrafish. International Journal of Molecular Sciences 20 (20):5116. doi: 10.3390/ijms20205116.
   PubMed Web of Science ®Google Scholar
• Moriguchi, T., M. Kita, Y. Tomono, T. Endo-Inagaki, and M. Omura. 1999. One type of chalcone synthase gene expressed during embryogenesis regulates the flavonoid accumulation in citrus cell cultures. Plant & Cell Physiology 40 (6):651–5. doi: 10.1093/oxfordjournals.pcp.a029589.
   PubMed Web of Science ®Google Scholar
• Muccilli, V., C. Licciardello, D. Fontanini, M. P. Russo, V. Cunsolo, R. Saletti, G. R. Recupero, and S. Foti.,. 2009. Proteome analysis of Citrus sinensis L. (Osbeck) flesh at ripening time. Journal of Proteomics 73 (1):134–52. doi: 10.1016/j.jprot.2009.09.005.
   PubMedGoogle Scholar
• Oh, C. J., J.-K. Woo, K. U. Yi, Y. C. Park, H.-Y. Lee, M. Kim, S. Park, S.-H. Yun, Y. Lee, H.-J. Kim, et al. 2021. Development of molecular markers for genotyping of Ruby, a locus controlling anthocyanin pigment production in citrus with its functional analysis. Scientia Horticulturae 289:110457. doi: http://dx.doi.org/10.1016/j.scienta.2021.110457.
   Web of Science ®Google Scholar
• Panche, A. N., A. D. Diwan, and S. R. Chandra. 2016. Flavonoids: An overview. Journal of Nutritional Science 5 (47):1–15. doi: 10.1017/jns.2016.4.
   Google Scholar
• Pannitteri, C., A. Continella, L. Lo Cicero, A. Gentile, S. La Malfa, E. Sperlinga, E. M. Napoli, T. Strano, G. Ruberto, and L. Siracusa. 2017. Influence of postharvest treatments on qualitative and chemical parameters of Tarocco blood orange fruits to be used for fresh chilled juice. Food Chemistry 230:441–7. doi: http://dx.doi.org/10.1016/j.foodchem.2017.03.041.
   PubMed Web of Science ®Google Scholar
• Rao, M. J., S. Wu, M. Duan, and L. Wang. 2021. Antioxidant metabolites in primitive, wild, and cultivated citrus and their role in stress tolerance. Molecules 26 (19):5801. doi: 10.3390/molecules26195801.
   PubMedGoogle Scholar
• Rapisarda, P., S. E. Bellomo, and S. Intelisano. 2001. Storage temperature effects on blood orange fruit quality. Journal of Agricultural and Food Chemistry 49 (7):3230–5. doi: 10.1021/jf010032l.
   PubMed Web of Science ®Google Scholar
• Ribeiro, A. P. D., A. G. Pereira, M. C. Todo, A. S. S. Fujimori, P. P. dos Santos, D. Dantas, A. A. Fernandes, S. G. Zanati, N. M. A. Hassimotto, L. A. M. Zornoff, et al. 2021. Pera’ orange (Citrus Sinensis) and ‘Moro’ orange juice (Citrus Sinensis (L.) Osbeck) attenuates left ventricular dysfunction and oxidative stress and improves myocardial energy metabolism in acute doxorubicin-induced cardiotoxicity in rats. Nutrition 91–92:111350. doi: 10.1016/j.nut.2021.111350.
   PubMed Web of Science ®Google Scholar
• Rodrigo, M. J., B. Alquézar, E. Alós, J. Lado, and L. Zacarías. 2013. Biochemical bases and molecular regulation of pigmentation in the peel of citrus fruit. Scientia Horticulturae 163:46–62. doi: 10.1016/j.scienta.2013.08.014.
   Web of Science ®Google Scholar
• Salamone, F., G. Li Volti, L. Titta, L. Puzzo, I. Barbagallo, F. La Delia, S. Zelber-Sagi, M. Malaguarnera, P. G. Pelicci, M. Giorgio, et al. 2012. Moro orange juice prevents fatty liver in mice. World Journal of Gastroenterology 18 (29):3862–8. doi: 10.3748/wjg.v18.i29.3862.
   PubMedGoogle Scholar
• Sharma, S., K. Barman, R. N. Prasad, and J. Sing. 2020. Chilling stress during postharvest storage of fruits and vegetables. In New Frontiers in Stress Management for Durable Agriculture, eds. Rakshit, M., Singh, H.B., Singh, A.K., Singh, U.S. and Fraceto, L., 75–100. Singapore: Springer Publishing. doi: 10.1007/978-981-15-1322-0_6.
   Google Scholar
• Sicilia, A., V. Catara, E. Scialò, and A. Lo Piero. 2021. Fungal infection induces anthocyanin biosynthesis and changes in DNA methylation configuration of blood orange [Citrus sinensis L. (Osbeck)]. Plants 10 (2):244. doi: 10.3390/plants10020244.
   Google Scholar
• Sicilia, A., E. Scialò, I. Puglisi, and A. Lo Piero. 2020. Anthocyanin biosynthesis and DNA methylation dynamics in sweet orange fruit [Citrus sinensis L. (Osbeck)] under cold stress. Journal of Agricultural and Food Chemistry 68 (26):7024–31. doi: 10.1021/acs.jafc.0c02360.
   PubMed Web of Science ®Google Scholar
• Silveira, A. Q., G. K. Z. S. Dourado, and T. B. Cesar. 2015. Red-fleshed sweet orange juice improves the risk factors for metabolic syndrome. International Journal of Food Sciences and Nutrition 66 (7):830–6. doi: 10.3109/09637486.2015.1093610.
   PubMed Web of Science ®Google Scholar
• Strano, M. C., S. D. Silvestro, M. Allegra, G. Russo, and M. Caruso. 2021. Effect of cold storage on the postharvest quality of different Tarocco sweet orange clonal selections. Scientia Horticulturae 285:110167. doi: 10.1016/j.scienta.2021.110167.
   Web of Science ®Google Scholar
• Sun, J., L. Lin, and P. Chen. 2013. Recent applications for HPLC-MS analysis of anthocyanins in food materials. Current Analytical Chemistry 9 (3):397–416. doi: 10.2174/1573411011309030009.
   Web of Science ®Google Scholar
• Sun, Q., L. Zheng, X. Wan-Peng, H. Shao-Lan, Y. Shi-Lai, L. Qiang, Z. Yong-Qiang, L. Deng, and X. Rang-Jin. 2014. Transcriptome analysis of blood orange (Citrus sinensis) following fruit bagging treatment by digital gene expression profiling. The Journal of Horticultural Science and Biotechnology 89 (4):397–407. doi: 10.1080/14620316.2014.11513098.
   Web of Science ®Google Scholar
• Tirnaz, S, and J. Batley. 2019. DNA methylation: Toward crop disease resistance improvement. Trends in Plant Science 24 (12):1137–50. doi: 10.1016/j.tplants.2019.08.007.
   PubMed Web of Science ®Google Scholar
• Tomasello, B., G. A. Malfa, A. La Mantia, N. Miceli, G. Sferrazzo, M. F. Taviano, C. Di Giacomo, M. Renis, and R. Acquaviva. 2021. Anti-adipogenic and anti-oxidant effects of a standardised extract of Moro blood oranges (Citrus sinensis (L.) Osbeck) during adipocyte differentiation of 3T3-L1 preadipocytes. Natural Product Research 35 (16):2660–7. doi: 10.1080/14786419.2019.1660337.
   PubMed Web of Science ®Google Scholar
• Trinei, M., A. Carpi, R. Menabo’, M. Storto, M. Fornari, A. Marinelli, S. Minardi, M. Riboni, F. Casciaro, F. DiLisa, et al. 2022. Dietary intake of cyanidin 3-glucoside induces a long-lasting cardioprotection from ischemia/reperfusion injury by altering the microbiota. The Journal of Nutritional Biochemistry 101:108921. doi: 10.1016/j.jnutbio.2021.108921.
   PubMed Web of Science ®Google Scholar
• Wang, J., J. Liu, K. Chen, H. Li, J. He, B. Guan, and L. He. 2016. Anthocyanin biosynthesis regulation in the fruit of Citrus sinensis cv. tarocco. Plant Molecular Biology Reporter 34 (6):1043–55. doi: 10.1007/s11105-016-0984-0.
   Web of Science ®Google Scholar
• Wang, J.-H., J.-J. Liu, K.-L. Chen, H.-W. Li, J. He, B. Guan, and L. He.,. 2017. Comparative transcriptome and proteome profiling of two Citrus sinensis cultivars during fruit development and ripening. BMC Genomics 18 (1):984. doi: 10.1186/s12864-017-4366-2.
   PubMedGoogle Scholar
• Wu, Q., J. Bai, X. Tao, W. Mou, Z. Luo, L. Mao, Z. Ban, T. Ying, and L. Li.,. 2018. Synergistic effect of abscisic acid and ethylene on color development in tomato (Solanum lycopersicum L.) fruit. Scientia Horticulturae 235:169–80. doi: 10.1016/j.scienta.2018.02.078.
   Web of Science ®Google Scholar
• Zhao, C. L., Z. J. Chen, X. S. Bai, C. Ding, T. J. Long, F. G. Wei, and K. R. Miao.,. 2014. Structure-activity relationships of anthocyanidin glycosylation. Molecular Diversity 18 (3):687–700. doi: 10.1007/s11030-014-9520-z.
   PubMed Web of Science ®Google Scholar