Induced pluripotent stem cell-based therapy for age-related macular degeneration

References

• Wong WL, Su X, Li X, et al. Global prevalence of age-related macular degeneration and disease burden projection for 2020 and 2040: a systematic review and meta-analysis. Lancet Glob Health. 2014;2(2):e106–16.
   PubMed Web of Science ®Google Scholar
• Martin DF, Maguire MG, Fine SL, et al. Ranibizumab and bevacizumab for treatment of neovascular age-related macular degeneration: two-year results. Ophthalmology. 2012;119(7):1388–1398.
   PubMed Web of Science ®Google Scholar
• Brown DM, Kaiser PK, Michels M, et al. Ranibizumab versus verteporfin for neovascular age-related macular degeneration. N Engl J Med. 2006;355(14):1432–1444.
   PubMed Web of Science ®Google Scholar
• Rosenfeld PJ, Brown DM, Heier JS, et al. Ranibizumab for neovascular age-related macular degeneration. N Engl J Med. 2006;355(14):1419–1431.
   PubMed Web of Science ®Google Scholar
• Singer MA, Awh CC, Sadda S, et al. HORIZON: an open-label extension trial of ranibizumab for choroidal neovascularization secondary to age-related macular degeneration. Ophthalmology. 2012;119(6):1175–1183.
   PubMed Web of Science ®Google Scholar
• Maguire MG, Martin DF, Ying GS, et al. Five-year outcomes with anti-vascular endothelial growth factor treatment of neovascular age-related macular degeneration: the comparison of age-related macular degeneration treatments trials. Ophthalmology. 2016;123(8):1751–1761.
   PubMed Web of Science ®Google Scholar
• Cohen SY, Mimoun G, Oubraham H, et al. Changes in visual acuity in patients with wet age-related macular degeneration treated with intravitreal ranibizumab in daily clinical practice: the LUMIERE study. Retina. 2013;33(3):474–481.
   PubMed Web of Science ®Google Scholar
• Hjelmqvist L, Lindberg C, Kanulf P, et al. One-year outcomes using ranibizumab for neovascular age-related macular degeneration: results of a prospective and retrospective observational multicentre study. J Ophthalmol. 2011;2011:405724.
   PubMed Web of Science ®Google Scholar
• Holz FG, Tadayoni R, Beatty S, et al. Multi-country real-life experience of anti-vascular endothelial growth factor therapy for wet age-related macular degeneration. Br J Ophthalmol. 2015;99(2):220–226.
   PubMed Web of Science ®Google Scholar
• Kruger Falk M, Kemp H, Sorensen TL. Four-year treatment results of neovascular age-related macular degeneration with ranibizumab and causes for discontinuation of treatment. Am J Ophthalmol. 2013;155(1):89–95.e3.
   PubMed Web of Science ®Google Scholar
• Rakic JM, Leys A, Brie H, et al. Real-world variability in ranibizumab treatment and associated clinical, quality of life, and safety outcomes over 24 months in patients with neovascular age-related macular degeneration: the HELIOS study. Clin Ophthalmol. 2013;7:1849–1858.
   PubMedGoogle Scholar
• Rasmussen A, Bloch SB, Fuchs J, et al. A 4-year longitudinal study of 555 patients treated with ranibizumab for neovascular age-related macular degeneration. Ophthalmology. 2013;120(12):2630–2636.
   PubMed Web of Science ®Google Scholar
• Souied EH, Oubraham H, Mimoun G, et al. Changes in visual acuity in patients with wet age-related macular degeneration treated with intravitreal ranibizumab in daily clinical practice: the TWIN Study. Retina. 2015;35(9):1743–1749.
   PubMed Web of Science ®Google Scholar
• Talks JS, Lotery AJ, Ghanchi F, et al. First-year visual acuity outcomes in the United Kingdom of providing aflibercept according to the VIEW study protocol for age-related macular degeneration. Ophthalmology. 2016;123(2):337–343.
   PubMed Web of Science ®Google Scholar
• Van Asten F, Evers-Birkenkamp KU, Van Lith-Verhoeven JJ, et al. A prospective, observational, open-label, multicentre study to investigate the daily treatment practice of ranibizumab in patients with neovascular age-related macular degeneration. Acta Ophthalmol. 2015;93(2):126–133.
   PubMed Web of Science ®Google Scholar
• Writing Committee for the UK Age-Related Macular Degeneration EMR Users Group. The neovascular age-related macular degeneration database: multicenter study of 92 976 ranibizumab injections: report 1: visual acuity. Ophthalmology. 2014;121(5):1092–1101.
   PubMed Web of Science ®Google Scholar
• Zarranz-Ventura J, Liew G, Johnston RL, et al. The neovascular age-related macular degeneration database: report 2: incidence, management, and visual outcomes of second treated eyes. Ophthalmology. 2014;121(10):1966–1975.
   PubMed Web of Science ®Google Scholar
• Ross AH, Donachie PH, Sallam A, et al. Which visual acuity measurements define high-quality care for patients with neovascular age-related macular degeneration treated with ranibizumab? Eye (Lond). 2013;27(1):56–64.
   PubMed Web of Science ®Google Scholar
• Arevalo JF, Lasave AF, Wu L, et al. Intravitreal bevacizumab for choroidal neovasculariztion in age-related macular degeneration: 5-year results of the pan-American collaborative retina study group. Retina. 2016;36(5):859–867.
   PubMed Web of Science ®Google Scholar
• Zarranz-Ventura J, Liew G, Johnston RL, et al. The neovascular age-related macular degeneration database: report 2: incidence, management, and visual outcomes of second treated eyes. Ophthalmology. 2014;121(10):1966–1975.
   PubMed Web of Science ®Google Scholar
• Mimeault M, Hauke R, Batra SK. Stem cells: a revolution in therapeutics-recent advances in stem cell biology and their therapeutic applications in regenerative medicine and cancer therapies. Clin Pharmacol Ther. 2007;82(3):252–264.
   PubMed Web of Science ®Google Scholar
• Zarbin MA. Current concepts in the pathogenesis of age-related macular degeneration. Arch Ophthalmol. 2004;122(4):598–614.
   PubMed Web of Science ®Google Scholar
• Ambati J, Ambati BK, Yoo SH, et al. Age-related macular degeneration: etiology, pathogenesis, and therapeutic strategies. Surv Ophthalmol. 2003;48(3):257–293.
   PubMed Web of Science ®Google Scholar
• Kinnunen K, Petrovski G, Moe MC, et al. Molecular mechanisms of retinal pigment epithelium damage and development of age-related macular degeneration. Acta Ophthalmol. 2012;90(4):299–309.
   PubMed Web of Science ®Google Scholar
• Eldred GE, Katz ML. Fluorophores of the human retinal pigment epithelium: separation and spectral characterization. Exp Eye Res. 1988;47(1):71–86.
   PubMed Web of Science ®Google Scholar
• Dorey CK, Wu G, Ebenstein D, et al. Cell loss in the aging retina. Relationship to lipofuscin accumulation and macular degeneration. Invest Ophthalmol Vis Sci. 1989;30(8):1691–1699.
   PubMed Web of Science ®Google Scholar
• Karwatowski WS, Jeffries TE, Duance VC, et al. Preparation of Bruch’s membrane and analysis of the age-related changes in the structural collagens. Br J Ophthalmol. 1995;79(10):944–952.
   PubMed Web of Science ®Google Scholar
• Ramrattan RS, Van Der Schaft TL, Mooy CM, et al. Morphometric analysis of Bruch’s membrane, the choriocapillaris, and the choroid in aging. Invest Ophthalmol Vis Sci. 1994;35(6):2857–2864.
   PubMed Web of Science ®Google Scholar
• Pauleikhoff D, Harper CA, Marshall J, et al. Aging changes in Bruch’s membrane. A histochemical and morphologic study. Ophthalmology. 1990;97(2):171–178.
   PubMed Web of Science ®Google Scholar
• Loffler KU, Lee WR. Basal linear deposit in the human macula. Graefes Arch Clin Exp Ophthalmol. 1986;224(6):493–501.
   PubMed Web of Science ®Google Scholar
• Chong NH, Keonin J, Luthert PJ, et al. Decreased thickness and integrity of the macular elastic layer of Bruch’s membrane correspond to the distribution of lesions associated with age-related macular degeneration. Am J Pathol. 2005;166(1):241–251.
   PubMed Web of Science ®Google Scholar
• Keenan TD, Pickford CE, Holley RJ, et al. Age-dependent changes in heparan sulfate in human Bruch’s membrane: implications for age-related macular degeneration. Invest Ophthalmol Vis Sci. 2014;55(8):5370–5379.
   PubMed Web of Science ®Google Scholar
• Wakatsuki Y, Shinojima A, Kawamura A, et al. Correlation of aging and segmental choroidal thickness measurement using swept source optical coherence tomography in healthy eyes. Plos One. 2015;10(12):e0144156.
   PubMed Web of Science ®Google Scholar
• Chirco KR, Sohn EH, Stone EM, et al. Structural and molecular changes in the aging choroid: implications for age-related macular degeneration. Eye (Lond). 2017;31(1):10–25.
   PubMed Web of Science ®Google Scholar
• Evans MD, Dizdaroglu M, Cooke MS. Oxidative DNA damage and disease: induction, repair and significance. Mutat Res. 2004;567(1):1–61.
   PubMed Web of Science ®Google Scholar
• Blasiak J, Petrovski G, Vereb Z, et al. Oxidative stress, hypoxia, and autophagy in the neovascular processes of age-related macular degeneration. Biomed Res Int. 2014;2014:768026.
   PubMed Web of Science ®Google Scholar
• Samiec PS, Drews-Botsch C, Flagg EW, et al. Glutathione in human plasma: decline in association with aging, age-related macular degeneration, and diabetes. Free Radic Biol Med. 1998;24(5):699–704.
   PubMed Web of Science ®Google Scholar
• Age-Related Eye Disease Study Research Group. A randomized, placebo-controlled, clinical trial of high-dose supplementation with vitamins C and E, beta carotene, and zinc for age-related macular degeneration and vision loss: AREDS report no. 8. Arch Ophthalmol. 2001;119(10):1417–1436.
   PubMed Web of Science ®Google Scholar
• Seddon JM, George S, Rosner B, et al. Progression of age-related macular degeneration: prospective assessment of C-reactive protein, interleukin 6, and other cardiovascular biomarkers. Arch Ophthalmol. 2005;123(6):774–782.
   PubMedGoogle Scholar
• Bok D. Evidence for an inflammatory process in age-related macular degeneration gains new support. Proc Natl Acad Sci U S A. 2005;102(20):7053–7054.
   PubMed Web of Science ®Google Scholar
• Grossniklaus HE, Miskala PH, Green WR, et al. Histopathologic and ultrastructural features of surgically excised subfoveal choroidal neovascular lesions: submacular surgery trials report no. 7. Arch Ophthalmol. 2005;123(7):914–921.
   PubMedGoogle Scholar
• Klein RJ, Zeiss C, Chew EY, et al. Complement factor H polymorphism in age-related macular degeneration. Science. 2005;308(5720):385–389.
   PubMed Web of Science ®Google Scholar
• Haines JL, Hauser MA, Schmidt S, et al. Complement factor H variant increases the risk of age-related macular degeneration. Science. 2005;308(5720):419–421.
   PubMed Web of Science ®Google Scholar
• Ambati J, Atkinson JP, Gelfand BD. Immunology of age-related macular degeneration. Nat Rev Immunol. 2013;13(6):438–451.
   PubMed Web of Science ®Google Scholar
• Khandhadia S, Cipriani V, Yates JR, et al. Age-related macular degeneration and the complement system. Immunobiology. 2012;217(2):127–146.
   PubMed Web of Science ®Google Scholar
• Van Lookeren Campagne M, LeCouter J, Yaspan BL, et al. Mechanisms of age-related macular degeneration and therapeutic opportunities. J Pathol. 2014;232(2):151–164.
   PubMed Web of Science ®Google Scholar
• Francis PJ, Schultz DW, Hamon S, et al. Haplotypes in the complement factor H (CFH) gene: associations with drusen and advanced age-related macular degeneration. Plos One. 2007;2(11):e1197.
   PubMed Web of Science ®Google Scholar
• Nozaki M, Raisler BJ, Sakurai E, et al. Drusen complement components C3a and C5a promote choroidal neovascularization. Proc Natl Acad Sci U S A. 2006;103(7):2328–2333.
   PubMed Web of Science ®Google Scholar
• Mullins RF, Schoo DP, Sohn EH, et al. The membrane attack complex in aging human choriocapillaris: relationship to macular degeneration and choroidal thinning. Am J Pathol. 2014;184(11):3142–3153.
   PubMed Web of Science ®Google Scholar
• Benzaquen LR, Nicholson-Weller A, Halperin JA. Terminal complement proteins C5b-9 release basic fibroblast growth factor and platelet-derived growth factor from endothelial cells. J Exp Med. 1994;179(3):985–992.
   PubMed Web of Science ®Google Scholar
• Bora PS, Sohn JH, Cruz JM, et al. Role of complement and complement membrane attack complex in laser-induced choroidal neovascularization. J Immunol. 2005;174(1):491–497.
   PubMed Web of Science ®Google Scholar
• Tsutsumi C, Sonoda KH, Egashira K, et al. The critical role of ocular-infiltrating macrophages in the development of choroidal neovascularization. J Leukoc Biol. 2003;74(1):25–32.
   PubMed Web of Science ®Google Scholar
• Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts. Science. 1998;282(5391):1145–1147.
   PubMed Web of Science ®Google Scholar
• Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006;126(4):663–676.
   PubMed Web of Science ®Google Scholar
• Takahashi K, Tanabe K, Ohnuki M, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007;131(5):861–872.
   PubMed Web of Science ®Google Scholar
• Yu J, Vodyanik MA, Smuga-Otto K, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science. 2007;318(5858):1917–1920.
   PubMed Web of Science ®Google Scholar
• Huangfu D, Osafune K, Maehr R, et al. Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2. Nat Biotechnol. 2008;26(11):1269–1275.
   PubMed Web of Science ®Google Scholar
• Mali P, Ye Z, Hommond HH, et al. Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts. Stem Cells. 2008;26(8):1998–2005.
   PubMed Web of Science ®Google Scholar
• Judson RL, Babiarz JE, Venere M, et al. Embryonic stem cell-specific microRNAs promote induced pluripotency. Nat Biotechnol. 2009;27(5):459–461.
   PubMed Web of Science ®Google Scholar
• Nakagawa M, Koyanagi M, Tanabe K, et al. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat Biotechnol. 2008;26(1):101–106.
   PubMed Web of Science ®Google Scholar
• Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors. Science. 2008;322(5903):949–953.
   PubMed Web of Science ®Google Scholar
• Yu J, Hu K, Smuga-Otto K, et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009;324(5928):797–801.
   PubMed Web of Science ®Google Scholar
• Stadtfeld M, Nagaya M, Utikal J, et al. Induced pluripotent stem cells generated without viral integration. Science. 2008;322(5903):945–949.
   PubMed Web of Science ®Google Scholar
• Fusaki N, Ban H, Nishiyama A, et al. Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome. Proc Jpn Acad Ser B Phys Biol Sci. 2009;85(8):348–362.
   PubMed Web of Science ®Google Scholar
• Woltjen K, Michael IP, Mohseni P, et al. piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature. 2009;458(7239):766–770.
   PubMed Web of Science ®Google Scholar
• Jia F, Wilson KD, Sun N, et al. A nonviral minicircle vector for deriving human iPS cells. Nat Methods. 2010;7(3):197–199.
   PubMed Web of Science ®Google Scholar
• Warren L, Manos PD, Ahfeldt T, et al. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell. 2010;7(5):618–630.
   PubMed Web of Science ®Google Scholar
• Malik N, Rao MS. A review of the methods for human iPSC derivation. Methods Mol Biol. 2013;997:23–33.
   PubMedGoogle Scholar
• Miyoshi N, Ishii H, Nagano H, et al. Reprogramming of mouse and human cells to pluripotency using mature microRNAs. Cell Stem Cell. 2011;8(6):633–638.
   PubMed Web of Science ®Google Scholar
• Kaji K, Norrby K, Paca A, et al. Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Nature. 2009;458(7239):771–775.
   PubMed Web of Science ®Google Scholar
• Lyssiotis CA, Foreman RK, Staerk J, et al. Reprogramming of murine fibroblasts to induced pluripotent stem cells with chemical complementation of Klf4. Proc Natl Acad Sci U S A. 2009;106(22):8912–8917.
   PubMed Web of Science ®Google Scholar
• Kim D, Kim CH, Moon JI, et al. Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins. Cell Stem Cell. 2009;4(6):472–476.
   PubMed Web of Science ®Google Scholar
• Strauss O. The retinal pigment epithelium in visual function. Physiol Rev. 2005;85(3):845–881.
   PubMed Web of Science ®Google Scholar
• Runkle EA, Antonetti DA. The blood-retinal barrier: structure and functional significance. Methods Mol Biol. 2011;686:133–148.
   PubMedGoogle Scholar
• Young RW, Bok D. Participation of the retinal pigment epithelium in the rod outer segment renewal process. J Cell Biol. 1969;42(2):392–403.
   PubMed Web of Science ®Google Scholar
• Baehr W, Wu SM, Bird AC, et al. The retinoid cycle and retina disease. Vision Res. 2003;43(28):2957–2958.
   PubMed Web of Science ®Google Scholar
• Holtkamp GM, Kijlstra A, Peek R, et al. Retinal pigment epithelium-immune system interactions: cytokine production and cytokine-induced changes. Prog Retin Eye Res. 2001;20(1):29–48.
   PubMed Web of Science ®Google Scholar
• Ishida K, Panjwani N, Cao Z, et al. Participation of pigment epithelium in ocular immune privilege. 3. Epithelia cultured from iris, ciliary body, and retina suppress T-cell activation by partially non-overlapping mechanisms. Ocul Immunol Inflamm. 2003;11(2):91–105.
   PubMed Web of Science ®Google Scholar
• Berger AS, Kaplan HJ, Clinical experience with the surgical removal of subfoveal neovascular membranes. Short-term postoperative results. Ophthalmology. 1992;996:969–975. discussion 975-6
   PubMed Web of Science ®Google Scholar
• Lambert HM, Capone A Jr., Aaberg TM, et al. Surgical excision of subfoveal neovascular membranes in age-related macular degeneration. Am J Ophthalmol. 1992;113(3):257–262.
   PubMed Web of Science ®Google Scholar
• Grossniklaus HE, Hutchinson AK, Capone A Jr., et al. Clinicopathologic features of surgically excised choroidal neovascular membranes. Ophthalmology. 1994;101(6):1099–1111.
   PubMed Web of Science ®Google Scholar
• Spraul CW, Lang GE, Grossniklaus HE, et al. Histologic and morphometric analysis of the choroid, Bruch’s membrane, and retinal pigment epithelium in postmortem eyes with age-related macular degeneration and histologic examination of surgically excised choroidal neovascular membranes. Surv Ophthalmol. 1999;44(Suppl 1):S10–32.
   PubMed Web of Science ®Google Scholar
• Castellarin AA, Nasir M, Sugino IK, et al. Progressive presumed choriocapillaris atrophy after surgery for age-related macular degeneration. Retina. 1998;18(2):143–149.
   PubMed Web of Science ®Google Scholar
• Algvere PV, Berglin L, Gouras P, et al. Transplantation of fetal retinal pigment epithelium in age-related macular degeneration with subfoveal neovascularization. Graefes Arch Clin Exp Ophthalmol. 1994;232(12):707–716.
   PubMed Web of Science ®Google Scholar
• Peyman GA, Blinder KJ, Paris CL, et al. A technique for retinal pigment epithelium transplantation for age-related macular degeneration secondary to extensive subfoveal scarring. Ophthalmic Surg. 1991;22(2):102–108.
   PubMedGoogle Scholar
• Algvere PV, Berglin L, Gouras P, et al. Transplantation of RPE in age-related macular degeneration: observations in disciform lesions and dry RPE atrophy. Graefes Arch Clin Exp Ophthalmol. 1997;235(3):149–158.
   PubMed Web of Science ®Google Scholar
• Pieramici DJ, De Juan E Jr., Fujii GY, et al. Limited inferior macular translocation for the treatment of subfoveal choroidal neovascularization secondary to age-related macular degeneration. Am J Ophthalmol. 2000;130(4):419–428.
   PubMed Web of Science ®Google Scholar
• Binder S, Stolba U, Krebs I, et al. Transplantation of autologous retinal pigment epithelium in eyes with foveal neovascularization resulting from age-related macular degeneration: a pilot study. Am J Ophthalmol. 2002;133(2):215–225.
   PubMed Web of Science ®Google Scholar
• Binder S, Krebs I, Hilgers RD, et al. Outcome of transplantation of autologous retinal pigment epithelium in age-related macular degeneration: a prospective trial. Invest Ophthalmol Vis Sci. 2004;45(11):4151–4160.
   PubMed Web of Science ®Google Scholar
• Joussen AM, Heussen FM, Joeres S, et al. Autologous translocation of the choroid and retinal pigment epithelium in age-related macular degeneration. Am J Ophthalmol. 2006;142(1):17–30.
   PubMed Web of Science ®Google Scholar
• Joussen AM, Joeres S, Fawzy N, et al. Autologous translocation of the choroid and retinal pigment epithelium in patients with geographic atrophy. Ophthalmology. 2007;114(3):551–560.
   PubMed Web of Science ®Google Scholar
• Lu Y, Han L, Wang C, et al. A comparison of autologous transplantation of retinal pigment epithelium (RPE) monolayer sheet graft with RPE-Bruch’s membrane complex graft in neovascular age-related macular degeneration. Acta Ophthalmol. 2016. doi:10.1111/aos.13054.
   Google Scholar
• Maaijwee K, Heimann H, Missotten T, et al. Retinal pigment epithelium and choroid translocation in patients with exudative age-related macular degeneration: long-term results. Graefes Arch Clin Exp Ophthalmol. 2007;245(11):1681–1689.
   PubMed Web of Science ®Google Scholar
• MacLaren RE, Uppal GS, Balaggan KS, et al. Autologous transplantation of the retinal pigment epithelium and choroid in the treatment of neovascular age-related macular degeneration. Ophthalmology. 2007;114(3):561–570.
   PubMed Web of Science ®Google Scholar
• Tezel TH, Del Priore LV, Berger AS, et al. Adult retinal pigment epithelial transplantation in exudative age-related macular degeneration. Am J Ophthalmol. 2007;143(4):584–595.
   PubMed Web of Science ®Google Scholar
• Stanga PE, Kychenthal A, Fitzke FW, et al. Retinal pigment epithelium translocation after choroidal neovascular membrane removal in age-related macular degeneration. Ophthalmology. 2002;109(8):1492–1498.
   PubMed Web of Science ®Google Scholar
• Akduman L, Karavellas MP, MacDonald JC, et al. Macular translocation with retinotomy and retinal rotation for exudative age-related macular degeneration. Retina. 1999;19(5):418–423.
   PubMed Web of Science ®Google Scholar
• Van Meurs JC, Ter Averst E, Hofland LJ, et al. Autologous peripheral retinal pigment epithelium translocation in patients with subfoveal neovascular membranes. Br J Ophthalmol. 2004;88(1):110–113.
   PubMed Web of Science ®Google Scholar
• Algvere PV, Gouras P, Dafgard Kopp E. Long-term outcome of RPE allografts in non-immunosuppressed patients with AMD. Eur J Ophthalmol. 1999;9(3):217–230.
   PubMed Web of Science ®Google Scholar
• Klimanskaya I, Hipp J, Rezai KA, et al. Derivation and comparative assessment of retinal pigment epithelium from human embryonic stem cells using transcriptomics. Cloning Stem Cells. 2004;6(3):217–245.
   PubMedGoogle Scholar
• Osakada F, Jin ZB, Hirami Y, et al. In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction. J Cell Sci. 2009;122(Pt 17):3169–3179.
   PubMed Web of Science ®Google Scholar
• Idelson M, Alper R, Obolensky A, et al. Directed differentiation of human embryonic stem cells into functional retinal pigment epithelium cells. Cell Stem Cell. 2009;5(4):396–408.
   PubMed Web of Science ®Google Scholar
• Kawasaki H, Suemori H, Mizuseki K, et al. Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity. Proc Natl Acad Sci U S A. 2002;99(3):1580–1585.
   PubMed Web of Science ®Google Scholar
• Zhang YS, Lu ZY, Yu Y, et al. Derivation, culture and retinal pigment epithelial differentiation of human embryonic stem cells using human fibroblast feeder cells. J Assist Reprod Genet. 2012;29(8):735–744.
   PubMed Web of Science ®Google Scholar
• Cho MS, Kim SJ, Ku SY, et al. Generation of retinal pigment epithelial cells from human embryonic stem cell-derived spherical neural masses. Stem Cell Res. 2012;9(2):101–109.
   PubMed Web of Science ®Google Scholar
• Maruotti J, Wahlin K, Gorrell D, et al. A simple and scalable process for the differentiation of retinal pigment epithelium from human pluripotent stem cells. Stem Cells Transl Med. 2013;2(5):341–354.
   PubMed Web of Science ®Google Scholar
• Buchholz DE, Hikita ST, Rowland TJ, et al. Derivation of functional retinal pigmented epithelium from induced pluripotent stem cells. Stem Cells. 2009;27(10):2427–2434.
   PubMed Web of Science ®Google Scholar
• Lane A, Philip LR, Ruban L, et al. Engineering efficient retinal pigment epithelium differentiation from human pluripotent stem cells. Stem Cells Transl Med. 2014;3(11):1295–1304.
   PubMed Web of Science ®Google Scholar
• Leach LL, Buchholz DE, Nadar VP, et al. Canonical/beta-catenin Wnt pathway activation improves retinal pigmented epithelium derivation from human embryonic stem cells. Invest Ophthalmol Vis Sci. 2015;56(2):1002–1013.
   PubMed Web of Science ®Google Scholar
• Maruotti J, Sripathi SR, Bharti K, et al. Small-molecule-directed, efficient generation of retinal pigment epithelium from human pluripotent stem cells. Proc Natl Acad Sci U S A. 2015;112(35):10950–10955.
   PubMed Web of Science ®Google Scholar
• Gong J, Fields MA, Moreira EF, et al. Differentiation of human protein-induced pluripotent stem cells toward a retinal pigment epithelial cell fate. Plos One. 2015;10(11):e0143272.
   PubMed Web of Science ®Google Scholar
• Choudhary P, Booth H, Gutteridge A, et al. Directing differentiation of pluripotent stem cells toward retinal pigment epithelium lineage. Stem Cells Transl Med. 2016;6(2):490–501.
   PubMed Web of Science ®Google Scholar
• Haruta M, Sasai Y, Kawasaki H, et al. In vitro and in vivo characterization of pigment epithelial cells differentiated from primate embryonic stem cells. Invest Ophthalmol Vis Sci. 2004;45(3):1020–1025.
   PubMed Web of Science ®Google Scholar
• Carr AJ, Vugler AA, Hikita ST, et al. Protective effects of human iPS-derived retinal pigment epithelium cell transplantation in the retinal dystrophic rat. Plos One. 2009;4(12):e8152.
   PubMed Web of Science ®Google Scholar
• Vugler A, Carr AJ, Lawrence J, et al. Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation. Exp Neurol. 2008;214(2):347–361.
   PubMed Web of Science ®Google Scholar
• Lund RD, Wang S, Klimanskaya I, et al. Human embryonic stem cell-derived cells rescue visual function in dystrophic RCS rats. Cloning Stem Cells. 2006;8(3):189–199.
   PubMedGoogle Scholar
• Sun J, Mandai M, Kamao H, et al. Protective effects of human iPS-derived retinal pigmented epithelial cells in comparison with human mesenchymal stromal cells and human neural stem cells on the degenerating retina in rd1 mice. Stem Cells. 2015;33(5):1543–1553.
   PubMed Web of Science ®Google Scholar
• Plaza Reyes A, Petrus-Reurer S, Antonsson L, et al. Xeno-free and defined human embryonic stem cell-derived retinal pigment epithelial cells functionally integrate in a large-eyed preclinical model. Stem Cell Reports. 2016;6(1):9–17.
   PubMed Web of Science ®Google Scholar
• Koss MJ, Falabella P, Stefanini FR, et al. Subretinal implantation of a monolayer of human embryonic stem cell-derived retinal pigment epithelium: a feasibility and safety study in Yucatan minipigs. Graefes Arch Clin Exp Ophthalmol. 2016;254(8):1553–1565.
   PubMed Web of Science ®Google Scholar
• Kokkinaki M, Sahibzada N, Golestaneh N. Human induced pluripotent stem-derived retinal pigment epithelium (RPE) cells exhibit ion transport, membrane potential, polarized vascular endothelial growth factor secretion, and gene expression pattern similar to native RPE. Stem Cells. 2011;29(5):825–835.
   PubMed Web of Science ®Google Scholar
• Sugino IK, Sun Q, Wang J, et al. Comparison of FRPE and human embryonic stem cell-derived RPE behavior on aged human Bruch’s membrane. Invest Ophthalmol Vis Sci. 2011;52(8):4979–4997.
   PubMed Web of Science ®Google Scholar
• Liao JL, Yu J, Huang K, et al. Molecular signature of primary retinal pigment epithelium and stem-cell-derived RPE cells. Hum Mol Genet. 2010;19(21):4229–4238.
   PubMed Web of Science ®Google Scholar
• Schwartz SD, Hubschman JP, Heilwell G, et al. Embryonic stem cell trials for macular degeneration: a preliminary report. Lancet. 2012;379(9817):713–720.
   PubMed Web of Science ®Google Scholar
• Schwartz SD, Regillo CD, Lam BL, et al. Human embryonic stem cell-derived retinal pigment epithelium in patients with age-related macular degeneration and Stargardt’s macular dystrophy: follow-up of two open-label phase 1/2 studies. Lancet. 2015;385(9967):509–516.
   PubMed Web of Science ®Google Scholar
• Schwartz SD, Tan G, Hosseini H, et al. Subretinal transplantation of embryonic stem cell-derived retinal pigment epithelium for the treatment of macular degeneration: an assessment at 4 years. Invest Ophthalmol Vis Sci. 2016;57(5):ORSFc1–9.
   PubMed Web of Science ®Google Scholar
• Song WK, Park KM, Kim HJ, et al. Treatment of macular degeneration using embryonic stem cell-derived retinal pigment epithelium: preliminary results in Asian patients. Stem Cell Reports. 2015;4(5):860–872.
   PubMed Web of Science ®Google Scholar
• Garber K. RIKEN suspends first clinical trial involving induced pluripotent stem cells. Nat Biotechnol. 2015;33(9):890–891.
   PubMed Web of Science ®Google Scholar
• Chakradhar S. An eye to the future: researchers debate best path for stem cell-derived therapies. Nat Med. 2016;22(2):116–119.
   PubMed Web of Science ®Google Scholar
• Foss AJ, Scott LJ, Rogers CA, et al. Changes in intraocular pressure in study and fellow eyes in the IVAN trial. Br J Ophthalmol. 2016.
   Web of Science ®Google Scholar
• Mandai M, Watanabe A, Kurimoto Y, et al. Autologous induced stem-cell-derived retinal cells for macular degeneration. N Engl J Med. 2017;376(11):1038–1046.
   PubMed Web of Science ®Google Scholar
• Yoshihara M, Hayashizaki Y, Murakawa Y. Genomic instability of iPSCs: challenges towards their clinical applications. Stem Cell Rev. 2016;13(1):7–16.
   Web of Science ®Google Scholar
• Zhao X, Liu J, Ahmad I. Differentiation of embryonic stem cells into retinal neurons. Biochem Biophys Res Commun. 2002;297(2):177–184.
   PubMed Web of Science ®Google Scholar
• Zhao X, Liu J, Ahmad I. Differentiation of embryonic stem cells to retinal cells in vitro. Methods Mol Biol. 2006;330:401–416.
   PubMedGoogle Scholar
• Yanai A, Laver C, Joe AW, et al. Efficient production of photoreceptor precursor cells from human embryonic stem cells. Methods Mol Biol. 2016;1307:357–369.
   PubMedGoogle Scholar
• Lamba DA, Karl MO, Ware CB, et al. Efficient generation of retinal progenitor cells from human embryonic stem cells. Proc Natl Acad Sci U S A. 2006;103(34):12769–12774.
   PubMed Web of Science ®Google Scholar
• Hirami Y, Osakada F, Takahashi K, et al. Generation of retinal cells from mouse and human induced pluripotent stem cells. Neurosci Lett. 2009;458(3):126–131.
   PubMed Web of Science ®Google Scholar
• Meyer JS, Shearer RL, Capowski EE, et al. Modeling early retinal development with human embryonic and induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2009;106(39):16698–16703.
   PubMed Web of Science ®Google Scholar
• Takahashi M, Palmer TD, Takahashi J, et al. Widespread integration and survival of adult-derived neural progenitor cells in the developing optic retina. Mol Cell Neurosci. 1998;12(6):340–348.
   PubMed Web of Science ®Google Scholar
• Guo Y, Saloupis P, Shaw SJ, et al. Engraftment of adult neural progenitor cells transplanted to rat retina injured by transient ischemia. Invest Ophthalmol Vis Sci. 2003;44(7):3194–3201.
   PubMed Web of Science ®Google Scholar
• Qiu G, Seiler MJ, Mui C, et al. Photoreceptor differentiation and integration of retinal progenitor cells transplanted into transgenic rats. Exp Eye Res. 2005;80(4):515–525.
   PubMed Web of Science ®Google Scholar
• Arnhold S, Klein H, Semkova I, et al. Neurally selected embryonic stem cells induce tumor formation after long-term survival following engraftment into the subretinal space. Invest Ophthalmol Vis Sci. 2004;45(12):4251–4255.
   PubMed Web of Science ®Google Scholar
• Schraermeyer U, Thumann G, Luther T, et al. Subretinally transplanted embryonic stem cells rescue photoreceptor cells from degeneration in the RCS rats. Cell Transplant. 2001;10(8):673–680.
   PubMed Web of Science ®Google Scholar
• Meyer JS, Katz ML, Maruniak JA, et al. Neural differentiation of mouse embryonic stem cells in vitro and after transplantation into eyes of mutant mice with rapid retinal degeneration. Brain Res. 2004;1014(1–2):131–144.
   PubMed Web of Science ®Google Scholar
• West EL, Gonzalez-Cordero A, Hippert C, et al. Defining the integration capacity of embryonic stem cell-derived photoreceptor precursors. Stem Cells. 2012;30(7):1424–1435.
   PubMed Web of Science ®Google Scholar
• Mizumoto H, Mizumoto K, Shatos MA, et al. Retinal transplantation of neural progenitor cells derived from the brain of GFP transgenic mice. Vision Res. 2003;43(16):1699–1708.
   PubMed Web of Science ®Google Scholar
• Pressmar S, Ader M, Richard G, et al. The fate of heterotopically grafted neural precursor cells in the normal and dystrophic adult mouse retina. Invest Ophthalmol Vis Sci. 2001;42(13):3311–3319.
   PubMed Web of Science ®Google Scholar
• Li T, Lewallen M, Chen S, et al. Multipotent stem cells isolated from the adult mouse retina are capable of producing functional photoreceptor cells. Cell Res. 2013;23(6):788–802.
   PubMed Web of Science ®Google Scholar
• Tucker BA, Park IH, Qi SD, et al. Transplantation of adult mouse iPS cell-derived photoreceptor precursors restores retinal structure and function in degenerative mice. Plos One. 2011;6(4):e18992.
   PubMed Web of Science ®Google Scholar
• Wang S, Girman S, Lu B, et al. Long-term vision rescue by human neural progenitors in a rat model of photoreceptor degeneration. Invest Ophthalmol Vis Sci. 2008;49(7):3201–3206.
   PubMed Web of Science ®Google Scholar
• McGill TJ, Cottam B, Lu B, et al. Transplantation of human central nervous system stem cells – neuroprotection in retinal degeneration. Eur J Neurosci. 2012;35(3):468–477.
   PubMed Web of Science ®Google Scholar
• Banin E, Obolensky A, Idelson M, et al. Retinal incorporation and differentiation of neural precursors derived from human embryonic stem cells. Stem Cells. 2006;24(2):246–257.
   PubMed Web of Science ®Google Scholar
• Young MJ, Ray J, Whiteley SJ, et al. Neuronal differentiation and morphological integration of hippocampal progenitor cells transplanted to the retina of immature and mature dystrophic rats. Mol Cell Neurosci. 2000;16(3):197–205.
   PubMed Web of Science ®Google Scholar
• Nishida A, Takahashi M, Tanihara H, et al. Incorporation and differentiation of hippocampus-derived neural stem cells transplanted in injured adult rat retina. Invest Ophthalmol Vis Sci. 2000;41(13):4268–4274.
   PubMed Web of Science ®Google Scholar
• Wojciechowski AB, Englund U, Lundberg C, et al. Subretinal transplantation of brain-derived precursor cells to young RCS rats promotes photoreceptor cell survival. Exp Eye Res. 2002;75(1):23–37.
   PubMed Web of Science ®Google Scholar
• Mandai M, Fujii M, Hashiguchi T, et al. iPSC-derived retina transplants improve vision in rd1 end-stage retinal-degeneration mice. Stem Cell Reports. 2017;8(1):69–83.
   PubMed Web of Science ®Google Scholar
• Chaudhry GR, Fecek C, Lai MM, et al. Fate of embryonic stem cell derivatives implanted into the vitreous of a slow retinal degenerative mouse model. Stem Cells Dev. 2009;18(2):247–258.
   PubMed Web of Science ®Google Scholar
• Lamba DA, McUsic A, Hirata RK, et al. Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells. Plos One. 2010;5(1):e8763.
   PubMed Web of Science ®Google Scholar
• Lamba DA, Gust J, Reh TA. Transplantation of human embryonic stem cell-derived photoreceptors restores some visual function in Crx-deficient mice. Cell Stem Cell. 2009;4(1):73–79.
   PubMed Web of Science ®Google Scholar
• Wang S, Lu B, Girman S, et al. Non-invasive stem cell therapy in a rat model for retinal degeneration and vascular pathology. Plos One. 2010;5(2):e9200.
   PubMed Web of Science ®Google Scholar
• Chao JR, Lamba DA, Klesert TR, et al. Transplantation of human embryonic stem cell-derived retinal cells into the subretinal space of a non-human primate. Transl Vis Sci Technol. 2017;6(3):4.
   PubMed Web of Science ®Google Scholar
• Santos-Ferreira T, Llonch S, Borsch O, et al. Retinal transplantation of photoreceptors results in donor-host cytoplasmic exchange. Nat Commun. 2016;7:13028.
   PubMed Web of Science ®Google Scholar
• Singh MS, Balmer J, Barnard AR, et al. Transplanted photoreceptor precursors transfer proteins to host photoreceptors by a mechanism of cytoplasmic fusion. Nat Commun. 2016;7:13537.
   PubMed Web of Science ®Google Scholar
• Pearson RA, Gonzalez-Cordero A, West EL, et al. Donor and host photoreceptors engage in material transfer following transplantation of post-mitotic photoreceptor precursors. Nat Commun. 2016;7:13029.
   PubMed Web of Science ®Google Scholar
• MacLaren RE, Pearson RA, MacNeil A, et al. Retinal repair by transplantation of photoreceptor precursors. Nature. 2006;444(7116):203–207.
   PubMed Web of Science ®Google Scholar
• Moisseiev E, Smit-McBride Z, Oltjen S, et al. Intravitreal administration of human bone marrow CD34+ stem cells in a murine model of retinal degeneration. Invest Ophthalmol Vis Sci. 2016;57(10):4125–4135.
   PubMed Web of Science ®Google Scholar
• Park SS, Moisseiev E, Bauer G, et al. Advances in bone marrow stem cell therapy for retinal dysfunction. Prog Retin Eye Res. 2016;56:148–165.
   PubMed Web of Science ®Google Scholar
• Kuriyan AE, Albini TA, Townsend JH, et al. Vision loss after intravitreal injection of autologous “stem cells” for AMD. N Engl J Med. 2017;376(11):1047–1053.
   PubMed Web of Science ®Google Scholar
• Kim JY, You YS, Kim SH, et al. Epiretinal membrane formation after intravitreal autologous stem cell implantation in a retinitis pigmentosa patient. Retin Cases Brief Rep. 2016;11(3):227–231.
   Google Scholar
• Leung EH, Flynn HW Jr., Albini TA, et al. Retinal detachment after subretinal stem cell transplantation. Ophthalmic Surg Lasers Imaging Retina. 2016;47(6):600–601.
   PubMed Web of Science ®Google Scholar
• Grisanti S, Ishioka M, Kosiewicz M, et al. Immunity and immune privilege elicited by cultured retinal pigment epithelial cell transplants. Invest Ophthalmol Vis Sci. 1997;38(8):1619–1626.
   PubMed Web of Science ®Google Scholar
• Zhang X, Bok D. Transplantation of retinal pigment epithelial cells and immune response in the subretinal space. Invest Ophthalmol Vis Sci. 1998;39(6):1021–1027.
   PubMed Web of Science ®Google Scholar
• Jiang LQ, Streilein JW. Immune responses elicited by transplantation and tissue-restricted antigens expressed on retinal tissues implanted subconjunctivally. Transplantation. 1991;52(3):513–519.
   PubMed Web of Science ®Google Scholar
• Drukker M. Immunogenicity of human embryonic stem cells: can we achieve tolerance? Springer Semin Immunopathol. 2004;26(1–2):201–213.
   PubMed Web of Science ®Google Scholar
• Zamiri P, Sugita S, Streilein JW. Immunosuppressive properties of the pigmented epithelial cells and the subretinal space. Chem Immunol Allergy. 2007;92:86–93.
   PubMedGoogle Scholar
• Jorgensen A, Wiencke AK, La Cour M, et al. Human retinal pigment epithelial cell-induced apoptosis in activated T cells. Invest Ophthalmol Vis Sci. 1998;39(9):1590–1599.
   PubMed Web of Science ®Google Scholar
• Zamiri P, Masli S, Streilein JW, et al. Pigment epithelial growth factor suppresses inflammation by modulating macrophage activation. Invest Ophthalmol Vis Sci. 2006;47(9):3912–3918.
   PubMed Web of Science ®Google Scholar
• Araki R, Uda M, Hoki Y, et al. Negligible immunogenicity of terminally differentiated cells derived from induced pluripotent or embryonic stem cells. Nature. 2013;494(7435):100–104.
   PubMed Web of Science ®Google Scholar
• Guha P, Morgan JW, Mostoslavsky G, et al. Lack of immune response to differentiated cells derived from syngeneic induced pluripotent stem cells. Cell Stem Cell. 2013;12(4):407–412.
   PubMed Web of Science ®Google Scholar
• Sugita S, Iwasaki Y, Makabe K, et al. Lack of T cell response to iPSC-derived retinal pigment epithelial cells from HLA homozygous donors. Stem Cell Reports. 2016;7(4):619–634.
   PubMed Web of Science ®Google Scholar
• Sugita S, Iwasaki Y, Makabe K, et al. Successful transplantation of retinal pigment epithelial cells from MHC homozygote iPSCs in MHC-matched models. Stem Cell Reports. 2016;7(4):635–648.
   PubMed Web of Science ®Google Scholar
• Okita K, Matsumura Y, Sato Y, et al. A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011;8(5):409–412.
   PubMed Web of Science ®Google Scholar
• Cai H, Del Priore LV. Bruch membrane aging alters the gene expression profile of human retinal pigment epithelium. Curr Eye Res. 2006;31(2):181–189.
   PubMed Web of Science ®Google Scholar
• Del Priore LV, Tezel TH. Reattachment rate of human retinal pigment epithelium to layers of human Bruch’s membrane. Arch Ophthalmol. 1998;116(3):335–341.
   PubMedGoogle Scholar
• Tezel TH, Del Priore LV. Reattachment to a substrate prevents apoptosis of human retinal pigment epithelium. Graefes Arch Clin Exp Ophthalmol. 1997;235(1):41–47.
   PubMed Web of Science ®Google Scholar
• Gullapalli VK, Sugino IK, Van Patten Y, et al. Impaired RPE survival on aged submacular human Bruch’s membrane. Exp Eye Res. 2005;80(2):235–248.
   PubMed Web of Science ®Google Scholar
• Lu L, Garcia CA, Mikos AG. Retinal pigment epithelium cell culture on thin biodegradable poly(DL-lactic-co-glycolic acid) films. J Biomater Sci Polym Ed. 1998;9(11):1187–1205.
   PubMed Web of Science ®Google Scholar
• Cai S, Smith ME, Redenti SM, et al. Mouse retinal progenitor cell dynamics on electrospun poly(-caprolactone). J Biomater Sci Polym Ed. 2012;23(11):1451–1465.
   PubMed Web of Science ®Google Scholar
• Tomita M, Lavik E, Klassen H, et al. Biodegradable polymer composite grafts promote the survival and differentiation of retinal progenitor cells. Stem Cells. 2005;23(10):1579–1588.
   PubMed Web of Science ®Google Scholar
• Lu B, Tai YC, Humayun MS. Microdevice-based cell therapy for age-related macular degeneration. Dev Ophthalmol. 2014;53:155–166.
   PubMedGoogle Scholar
• Warnke PH, Alamein M, Skabo S, et al. Primordium of an artificial Bruch’s membrane made of nanofibers for engineering of retinal pigment epithelium cell monolayers. Acta Biomater. 2013;9(12):9414–9422.
   PubMed Web of Science ®Google Scholar
• Lawley E, Baranov P, Young M. Hybrid vitronectin-mimicking polycaprolactone scaffolds for human retinal progenitor cell differentiation and transplantation. J Biomater Appl. 2015;29(6):894–902.
   PubMed Web of Science ®Google Scholar
• Yao J, Ko CW, Baranov PY, et al. Enhanced differentiation and delivery of mouse retinal progenitor cells using a micropatterned biodegradable thin-film polycaprolactone scaffold. Tissue Eng Part A. 2015;21(7–8):1247–1260.
   PubMed Web of Science ®Google Scholar
• Calejo MT, Ilmarinen T, Jongprasitkul H, et al. Honeycomb porous films as permeable scaffold materials for human embryonic stem cell-derived retinal pigment epithelium. J Biomed Mater Res A. 2016;104(7):1646–1656.
   PubMed Web of Science ®Google Scholar
• Peng CH, Chuang JH, Wang ML, et al. Laminin modification subretinal bio-scaffold remodels retinal pigment epithelium-driven microenvironment in vitro and in vivo. Oncotarget. 2016;7(40):64631–64648.
   PubMed Web of Science ®Google Scholar
• Gullapalli VK, Sugino IK, Zarbin MA. Culture-induced increase in alpha integrin subunit expression in retinal pigment epithelium is important for improved resurfacing of aged human Bruch’s membrane. Exp Eye Res. 2008;86(2):189–200.
   PubMed Web of Science ®Google Scholar
• Fang IM, Yang CH, Yang CM, et al. Overexpression of integrin alpha6 and beta4 enhances adhesion and proliferation of human retinal pigment epithelial cells on layers of porcine Bruch’s membrane. Exp Eye Res. 2009;88(1):12–21.
   PubMed Web of Science ®Google Scholar
• Sugino IK, Rapista A, Sun Q, et al. A method to enhance cell survival on Bruch’s membrane in eyes affected by age and age-related macular degeneration. Invest Ophthalmol Vis Sci. 2011;52(13):9598–9609.
   PubMed Web of Science ®Google Scholar
• Tezel TH, Del Priore LV, Kaplan HJ. Reengineering of aged Bruch’s membrane to enhance retinal pigment epithelium repopulation. Invest Ophthalmol Vis Sci. 2004;45(9):3337–3348.
   PubMed Web of Science ®Google Scholar
• Songstad AE, Wiley LA, Duong K, et al. Generating iPSC-derived choroidal endothelial cells to study age-related macular degeneration. Invest Ophthalmol Vis Sci. 2015;56(13):8258–8267.
   PubMed Web of Science ®Google Scholar