3D bioprinted scaffolds of polysaccharide hydrogels in osteochondral and cartilage tissue engineering

References

• Kon E, Delcogliano M, Filardo G, et al. Novel nano-composite multilayered biomaterial for osteochondral regeneration: a pilot clinical trial. Am J Sports Med. 2011;39(6):1180–1190. doi: 10.1177/0363546510392711
   PubMed Web of Science ®Google Scholar
• Gomoll AH, Madry H, Knutsen G, et al. The subchondral bone in articular cartilage repair: current problems in the surgical management. Knee Surg Sports Traumatol Arthrosc. 2010;18(4):434–447. doi: 10.1007/s00167-010-1072-x
   PubMed Web of Science ®Google Scholar
• Huey DJ, Hu JC, Athanasiou KA. Unlike bone, cartilage regeneration remains elusive. Science. 2012;338(6109):917–921. doi: 10.1126/science.1222454
   PubMed Web of Science ®Google Scholar
• Akter F, Ibanez J. Chapter 8 - bone and cartilage tissue engineering. In: Akter F, editor. Tissue engineering made easy. Academic Press; 2016. pp. 77–97. doi: 10.1016/B978-0-12-805361-4.00008-4.
   Google Scholar
• Ando W, Tateishi K, Hart DA, et al. Cartilage repair using an in vitro generated scaffold-free tissue-engineered construct derived from porcine synovial mesenchymal stem cells. Biomaterials. 2007;28(36):5462–5470. doi: 10.1016/j.biomaterials.2007.08.030
   PubMed Web of Science ®Google Scholar
• Lemoine M, Casey SM, O’Byrne JM, et al. The development of natural polymer scaffold-based therapeutics for osteochondral repair. Biochem Soc Trans. 2020;48(4):1433–1445. doi: 10.1042/BST20190938
   PubMed Web of Science ®Google Scholar
• Wei W, Ma Y, Yao X, et al. Advanced hydrogels for the repair of cartilage defects and regeneration. Bioact Mater. 2021;6(4):998–1011. doi: 10.1016/j.bioactmat.2020.09.030
   PubMed Web of Science ®Google Scholar
• Yilmaz B, Al Rashid A, Mou YA, et al. Bioprinting: a review of processes, materials and applications. Bioprinting. 2021;23:e00148. doi: 10.1016/j.bprint.2021.e00148
   Google Scholar
• Xie JH, Jin ML, Morris GA, et al. Advances on bioactive polysaccharides from medicinal plants. Crit Rev Food Sci Nutr. 2016;56(Suppl 1):S60–84. doi: 10.1080/10408398.2015.1069255
   PubMed Web of Science ®Google Scholar
• Shi L. Bioactivities, isolation and purification methods of polysaccharides from natural products: a review. Int j biol macromol. 2016;92:37–48. doi: 10.1016/j.ijbiomac.2016.06.100
   PubMed Web of Science ®Google Scholar
• Zhu T, Mao J, Cheng Y, et al. Recent progress of polysaccharide-based hydrogel interfaces for wound healing and tissue engineering. Adv Mater Interfaces. 2019;6(17):1900761. doi: 10.1002/admi.201900761
   Web of Science ®Google Scholar
• Duarte Campos DF, Blaeser A, Weber M, et al. Three-dimensional printing of stem cell-laden hydrogels submerged in a hydrophobic high-density fluid. Biofabrication. 2013;5(1):015003. doi: 10.1088/1758-5082/5/1/015003
   PubMed Web of Science ®Google Scholar
• Sadeghianmaryan A, Naghieh S, Alizadeh Sardroud H, et al. Extrusion-based printing of chitosan scaffolds and their in vitro characterization for cartilage tissue engineering. Int j biol macromol. 2020;164:3179–3192. doi: 10.1016/j.ijbiomac.2020.08.180
   PubMed Web of Science ®Google Scholar
• Ye K, Felimban R, Traianedes K, et al. Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold. PLoS One. 2014;9(6):e99410. doi: 10.1371/journal.pone.0099410
   PubMed Web of Science ®Google Scholar
• He Y, Derakhshanfar S, Zhong W, et al. Characterization and application of carboxymethyl chitosan-based bioink in cartilage tissue engineering. J Nanomater. 2020;2020:2057097. doi: 10.1155/2020/2057097
   Web of Science ®Google Scholar
• Pescosolido L, Schuurman W, Malda J, et al. Hyaluronic acid and dextran-based semi-IPN hydrogels as biomaterials for bioprinting. Biomacromolecules. 2011;12(5):1831–1838. doi: 10.1021/bm200178w
   PubMed Web of Science ®Google Scholar
• Daly AC, Critchley SE, Rencsok EM, et al. A comparison of different bioinks for 3D bioprinting of fibrocartilage and hyaline cartilage. Biofabrication. 2016;8(4):045002. doi: 10.1088/1758-5090/8/4/045002
   PubMed Web of Science ®Google Scholar
• Levato R, Webb WR, Otto IA, et al. The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells. Acta Biomaterialia. 2017;61:41–53. doi: 10.1016/j.actbio.2017.08.005
   PubMed Web of Science ®Google Scholar
• Kesti M, Müller M, Becher J, et al. A versatile bioink for three-dimensional printing of cellular scaffolds based on thermally and photo-triggered tandem gelation. Acta Biomaterialia. 2015;11:162–172. doi: 10.1016/j.actbio.2014.09.033
   PubMed Web of Science ®Google Scholar
• Park JY, Choi JC, Shim JH, et al. A comparative study on collagen type I and hyaluronic acid dependent cell behavior for osteochondral tissue bioprinting. Biofabrication. 2014;6(3):035004. doi: 10.1088/1758-5082/6/3/035004
   PubMed Web of Science ®Google Scholar
• Schuurman W, Levett PA, Pot MW, et al. Gelatin-methacrylamide hydrogels as potential biomaterials for fabrication of tissue-engineered cartilage constructs. Macromol biosci. 2013;13(5):551–561. doi: 10.1002/mabi.201200471
   PubMed Web of Science ®Google Scholar
• López-Marcial GR, Zeng AY, Osuna C, et al. O’Connell GD: agarose-based hydrogels as suitable bioprinting materials for tissue engineering. ACS Biomater Sci Eng. 2018;4(10):3610–3616. doi: 10.1021/acsbiomaterials.8b00903
   PubMed Web of Science ®Google Scholar
• Duarte Campos DF, Blaeser A, Korsten A, et al. The stiffness and structure of three-dimensional printed hydrogels direct the differentiation of mesenchymal stromal cells toward adipogenic and osteogenic lineages. Tissue Eng Part A. 2015;21(3–4):740–756. doi: 10.1089/ten.tea.2014.0231
   PubMed Web of Science ®Google Scholar
• Henrionnet C, Pourchet L, Neybecker P, et al. Combining innovative bioink and low cell density for the production of 3D-Bioprinted cartilage substitutes: a pilot study. Stem Cells Int. 2020;2020:2487072. doi: 10.1155/2020/2487072
   PubMed Web of Science ®Google Scholar
• Park S-H, Song T, Bae TS, et al. Synergistic effects of alginate coating method on cartilage tissue engineering using fibrin/ha composite gel. Int J Precis Eng Manuf. 2012;13(11):2067–2074. doi: 10.1007/s12541-012-0272-3
   Web of Science ®Google Scholar
• Müller M, Öztürk E, Arlov Ø, et al. Alginate Sulfate-Nanocellulose Bioinks for Cartilage Bioprinting Applications. Ann Biomed Eng. 2017;45(1):210–223. doi: 10.1007/s10439-016-1704-5
   PubMed Web of Science ®Google Scholar
• Markstedt K, Mantas A, Tournier I, et al. 3D bioprinting human chondrocytes with Nanocellulose-Alginate Bioink for cartilage tissue engineering applications. Biomacromolecules. 2015;16(5):1489–1496. doi: 10.1021/acs.biomac.5b00188
   PubMed Web of Science ®Google Scholar
• Huang J, Huang Z, Liang Y, et al. 3D printed gelatin/hydroxyapatite scaffolds for stem cell chondrogenic differentiation and articular cartilage repair. Biomater Sci. 2021;9(7):2620–2630. doi: 10.1039/D0BM02103B
   PubMed Web of Science ®Google Scholar
• Fan Y, Shi T, Yue X, et al. 3D Composite Cell Printing Gelatin/Sodium Alginate/n-HAP Bioscaffold. J Phys. 2019;1213(4):042020. doi: 10.1088/1742-6596/1213/4/042020
   Google Scholar
• Antich C, de Vicente J, Jiménez G, et al. Bio-inspired hydrogel composed of hyaluronic acid and alginate as a potential bioink for 3D bioprinting of articular cartilage engineering constructs. Acta Biomaterialia. 2020;106:114–123. doi: 10.1016/j.actbio.2020.01.046
   PubMed Web of Science ®Google Scholar
• Hodder E, Duin S, Kilian D, et al. Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes. J Mater Sci. 2019;30(1):10. doi: 10.1007/s10856-018-6211-9
   Web of Science ®Google Scholar
• Nguyen D, Hägg DA, Forsman A, et al. Cartilage tissue engineering by the 3D bioprinting of iPS Cells in a Nanocellulose/alginate bioink. Sci Rep. 2017;7(1):658. doi: 10.1038/s41598-017-00690-y
   PubMed Web of Science ®Google Scholar
• Ahmad Raus R, Wan Nawawi WMF, Nasaruddin RR. Alginate and alginate composites for biomedical applications. Asian J Pharm Sci. 2021;16(3):280–306. doi: 10.1016/j.ajps.2020.10.001
   PubMed Web of Science ®Google Scholar
• Trica B, Delattre C, Gros F, et al. Extraction and characterization of alginate from an edible brown seaweed (cystoseira barbata) harvested in the Romanian black sea. Mar Drugs. 2019;17(7):405. doi: 10.3390/md17070405
   PubMed Web of Science ®Google Scholar
• Urtuvia V, Maturana N, Acevedo F, et al. Bacterial alginate production: an overview of its biosynthesis and potential industrial production. World J Microbiol Biotechnol. 2017;33(11):198. doi: 10.1007/s11274-017-2363-x
   PubMed Web of Science ®Google Scholar
• Dudun AA, Akoulina EA, Zhuikov VA, et al. Competitive biosynthesis of bacterial alginate using Azotobacter vinelandii 12 for tissue engineering applications. Polymers. 2021;14(1). doi: 10.3390/polym14010131
   PubMed Web of Science ®Google Scholar
• Yan J, Miao Y, Tan H, et al. Injectable alginate/hydroxyapatite gel scaffold combined with gelatin microspheres for drug delivery and bone tissue engineering. Mater Sci Eng C. 2016;63:274–284. doi: 10.1016/j.msec.2016.02.071
   PubMed Web of Science ®Google Scholar
• Gonzalez-Fernandez T, Tenorio AJ, Campbell KT, et al. Alginate-based bioinks for 3D bioprinting and fabrication of anatomically accurate bone grafts. Tissue Eng Part A. 2021;27(17–18):1168–1181. doi: 10.1089/ten.tea.2020.0305
   PubMed Web of Science ®Google Scholar
• Choe G, Oh S, Seok JM, et al. Graphene oxide/alginate composites as novel bioinks for three-dimensional mesenchymal stem cell printing and bone regeneration applications. Nanoscale. 2019;11(48):23275–23285. doi: 10.1039/C9NR07643C
   PubMed Web of Science ®Google Scholar
• De Santis MM, Alsafadi HN, Tas S, et al. Extracellular-Matrix-Reinforced Bioinks for 3D Bioprinting Human Tissue. Adv Mater (Deerfield Beach, Fla). 2021;33(3):e2005476. doi: 10.1002/adma.202005476
   PubMed Web of Science ®Google Scholar
• Kang ES, Kim H, Han Y, et al. Enhancing osteogenesis of adipose-derived mesenchymal stem cells using gold nanostructure/peptide-nanopatterned graphene oxide. Colloids Surf B Biointerfaces. 2021;204:111807. doi: 10.1016/j.colsurfb.2021.111807
   PubMed Web of Science ®Google Scholar
• Zhang J, Wehrle E, Adamek P, et al. Optimization of mechanical stiffness and cell density of 3D bioprinted cell-laden scaffolds improves extracellular matrix mineralization and cellular organization for bone tissue engineering. Acta Biomaterialia. 2020;114:307–322. doi: 10.1016/j.actbio.2020.07.016
   PubMed Web of Science ®Google Scholar
• Gomez CG, Rinaudo M, Villar MA. Oxidation of sodium alginate and characterization of the oxidized derivatives. Carbohydr Polym. 2007;67(3):296–304. doi: 10.1016/j.carbpol.2006.05.025
   Web of Science ®Google Scholar
• Wei Z, Yang JH, Liu ZQ, et al. Novel biocompatible polysaccharide-based self-healing hydrogel. Adv Funct Mater. 2015;25(9):1352–1359. doi: 10.1002/adfm.201401502
   Web of Science ®Google Scholar
• Balakrishnan B, Jayakrishnan A. Self-cross-linking biopolymers as injectable in situ forming biodegradable scaffolds. Biomaterials. 2005;26(18):3941–3951. doi: 10.1016/j.biomaterials.2004.10.005
   PubMed Web of Science ®Google Scholar
• Distler T, Solisito AA, Schneidereit D, et al. 3D printed oxidized alginate-gelatin bioink provides guidance for C2C12 muscle precursor cell orientation and differentiation via shear stress during bioprinting. Biofabrication. 2020;12(4):045005. doi: 10.1088/1758-5090/ab98e4
   PubMed Web of Science ®Google Scholar
• Tabata M, Shimoda T, Sugihara K, et al. Osteoconductive and hemostatic properties of apatite formed on/in agarose gel as a bone-grafting material. J Biomed Mater Res, Part B. 2003;67(2):680–688. doi: 10.1002/jbm.b.10063
   PubMedGoogle Scholar
• Zarrintaj P, Manouchehri S, Ahmadi Z, et al. Agarose-based biomaterials for tissue engineering. Carbohydr Polym. 2018;187:66–84. doi: 10.1016/j.carbpol.2018.01.060
   PubMed Web of Science ®Google Scholar
• López-Marcial GR, Elango K, O’Connell GD. O’Connell GD: addition of collagen type I in agarose created a dose-dependent effect on matrix production in engineered cartilage. Regen Biomater. 2022;9:rbac048. doi: 10.1093/rb/rbac048
   PubMed Web of Science ®Google Scholar
• Yang J, Zhang YS, Yue K, et al. Cell-laden hydrogels for osteochondral and cartilage tissue engineering. Acta Biomaterialia. 2017;57:1–25. doi: 10.1016/j.actbio.2017.01.036
   PubMed Web of Science ®Google Scholar
• Kreimendahl F, Köpf M, Thiebes AL, et al. Three-dimensional printing and angiogenesis: tailored agarose-type I collagen blends comprise three-dimensional printability and angiogenesis potential for tissue-engineered substitutes. Tissue Eng Part C Methods. 2017;23(10):604–615. doi: 10.1089/ten.tec.2017.0234
   PubMed Web of Science ®Google Scholar
• Duarte Campos DF, Drescher W, Rath B, et al. Supporting biomaterials for articular cartilage repair. Cartilage. 2012;3(3):205–221. doi: 10.1177/1947603512444722
   PubMed Web of Science ®Google Scholar
• Singh YP, Bhardwaj N, Mandal BB. Potential of agarose/silk fibroin blended hydrogel for in vitro cartilage tissue engineering. ACS Appl Mater Inter. 2016;8(33):21236–21249. doi: 10.1021/acsami.6b08285
   PubMed Web of Science ®Google Scholar
• Melke J, Midha S, Ghosh S, et al. Silk fibroin as biomaterial for bone tissue engineering. Acta Biomaterialia. 2016;31:1–16. doi: 10.1016/j.actbio.2015.09.005
   PubMed Web of Science ®Google Scholar
• García-Martínez L, Campos F, Godoy-Guzmán C, et al. Encapsulation of human elastic cartilage-derived chondrocytes in nanostructured fibrin-agarose hydrogels. Histochem Cell Biol. 2017;147(1):83–95. doi: 10.1007/s00418-016-1485-9
   PubMed Web of Science ®Google Scholar
• Wu H, Wan Y, Cao X, et al. Proliferation of chondrocytes on porous poly(dl-lactide)/chitosan scaffolds. Acta Biomaterialia. 2008;4(1):76–87. doi: 10.1016/j.actbio.2007.06.010
   PubMed Web of Science ®Google Scholar
• Pranantyo D, Xu LQ, Kang ET, et al. Chitosan-based peptidopolysaccharides as cationic antimicrobial agents and antibacterial coatings. Biomacromolecules. 2018;19(6):2156–2165. doi: 10.1021/acs.biomac.8b00270
   PubMed Web of Science ®Google Scholar
• Bajorath J, Greenfield B, Munro SB, et al. Identification of CD44 residues important for hyaluronan binding and delineation of the binding site. J Biol Chem. 1998;273(1):338–343. doi: 10.1074/jbc.273.1.338
   PubMed Web of Science ®Google Scholar
• Mohan N, Mohanan PV, Sabareeswaran A, et al. Chitosan-hyaluronic acid hydrogel for cartilage repair. Int J Biol Macromol. 2017;104(Pt B):1936–1945. doi: 10.1016/j.ijbiomac.2017.03.142
   PubMed Web of Science ®Google Scholar
• Rampichová M, Filová E, Varga F, et al. Fibrin/hyaluronic acid composite hydrogels as appropriate scaffolds for in vivo artificial cartilage implantation. ASAIO J. 2010;56(6):563–568. doi: 10.1097/MAT.0b013e3181fcbe24
   PubMed Web of Science ®Google Scholar
• Li T, Chen C, Brozena AH, et al. Developing fibrillated cellulose as a sustainable technological material. Nature. 2021;590(7844):47–56. doi: 10.1038/s41586-020-03167-7
   PubMed Web of Science ®Google Scholar
• Yang X, Bakaic E, Hoare T, et al. Injectable polysaccharide hydrogels reinforced with cellulose nanocrystals: morphology, rheology, degradation, and cytotoxicity. Biomacromolecules. 2013;14(12):4447–4455. doi: 10.1021/bm401364z
   PubMed Web of Science ®Google Scholar
• Zhao J, Zheng K, Nan J, et al. Synthesis and characterization of lignosulfonate-graft-poly (acrylic acid)/hydroxyethyl cellulose semi-interpenetrating hydrogels. React Funct Polym. 2017;115:28–35. doi: 10.1016/j.reactfunctpolym.2017.03.019
   Web of Science ®Google Scholar
• Rocha PM, Santo VE, Gomes ME, et al. Encapsulation of adipose-derived stem cells and transforming growth factor-β1 in carrageenan-based hydrogels for cartilage tissue engineering. J Bioact Compat Polym. 2011;26(5):493–507. doi: 10.1177/0883911511420700
   Web of Science ®Google Scholar
• Dev A, Mohanbhai SJ, Kushwaha AC, et al. κ-carrageenan-C-phycocyanin based smart injectable hydrogels for accelerated wound recovery and real-time monitoring. Acta Biomaterialia. 2020;109:121–131. doi: 10.1016/j.actbio.2020.03.023
   PubMed Web of Science ®Google Scholar
• Ali KA, Hassan ME, Elnashar MMM. Development of functionalized carrageenan, chitosan and alginate as polymeric chelating ligands for water softening. Int J Environ Sci Technol. 2017;14(9):2009–2014. doi: 10.1007/s13762-017-1298-y
   Web of Science ®Google Scholar
• Kim UJ, Park J, Li C, et al. Structure and properties of silk hydrogels. Biomacromolecules. 2004;5(3):786–792. doi: 10.1021/bm0345460
   PubMed Web of Science ®Google Scholar
• Gholamipour-Shirazi A, Norton IT, Mills T. Dual stimuli-sensitive carrageenan-based formulation for additive manufacturing. Int J Biol macromol. 2021;189:370–379. doi: 10.1016/j.ijbiomac.2021.08.127
   PubMed Web of Science ®Google Scholar
• Cassimjee H, Kumar P, Choonara YE, et al. Proteosaccharide combinations for tissue engineering applications. Carbohydr Polym. 2020;235:115932. doi: 10.1016/j.carbpol.2020.115932
   PubMed Web of Science ®Google Scholar
• Sabzevari A, Rayat Pisheh H, Ansari M, et al. Progress in bioprinting technology for tissue regeneration. J Artif Organs. 2023;26(4):255–274. doi: 10.1007/s10047-023-01394-z
   PubMed Web of Science ®Google Scholar
• Unagolla JM, Jayasuriya AC. Hydrogel-based 3D bioprinting: a comprehensive review on cell-laden hydrogels, bioink formulations, and future perspectives. Appl Mater Today. 2020;18:18. doi: 10.1016/j.apmt.2019.100479
   Web of Science ®Google Scholar
• Hutmacher DW. Scaffolds in tissue engineering bone and cartilage. Biomaterials. 2000;21(24):2529–2543. doi: 10.1016/S0142-9612(00)00121-6
   PubMed Web of Science ®Google Scholar
• Hollister SJ. Porous scaffold design for tissue engineering. Nature Mater. 2005;4(7):518–524. doi: 10.1038/nmat1421
   PubMed Web of Science ®Google Scholar
• Fu J, In Het Panhuis M. In Het Panhuis M: Hydrogel properties and applications. J Mat Chem B. 2019;7(10):1523–1525. doi: 10.1039/C9TB90023C
   PubMed Web of Science ®Google Scholar
• You J, Cao J, Zhao Y, et al. Improved Mechanical Properties and Sustained Release Behavior of Cationic Cellulose Nanocrystals Reinforeced Cationic Cellulose Injectable Hydrogels. Biomacromolecules. 2016;17(9):2839–2848. doi: 10.1021/acs.biomac.6b00646
   PubMed Web of Science ®Google Scholar
• Pérez-Madrigal MM, Shaw JE, Arno MC, et al. Robust alginate/hyaluronic acid thiol-yne click-hydrogel scaffolds with superior mechanical performance and stability for load-bearing soft tissue engineering. Biomater Sci. 2020;8(1):405–412. doi: 10.1039/C9BM01494B
   PubMed Web of Science ®Google Scholar
• Olate-Moya F, Arens L, Wilhelm M, et al. Chondroinductive alginate-based hydrogels having graphene oxide for 3D printed scaffold fabrication. ACS Appl Mater Inter. 2020;12(4):4343–4357. doi: 10.1021/acsami.9b22062
   PubMed Web of Science ®Google Scholar
• Xu X, Xu L, Xia J, et al. Harnessing knee joint resident mesenchymal stem cells in cartilage tissue engineering. Acta Biomaterialia. 2023;168:372–387. doi: 10.1016/j.actbio.2023.07.024
   PubMed Web of Science ®Google Scholar
• Huang J, Liu Q, Xia J, et al. Modification of mesenchymal stem cells for cartilage-targeted therapy. J Transl Med. 2022;20(1):515. doi: 10.1186/s12967-022-03726-8
   PubMed Web of Science ®Google Scholar
• Jia Z, Liu Q, Liang Y, et al. Repair of articular cartilage defects with intra-articular injection of autologous rabbit synovial fluid-derived mesenchymal stem cells. J Transl Med. 2018;16(1):123. doi: 10.1186/s12967-018-1485-8
   PubMedGoogle Scholar
• Nakamura A, Murata D, Fujimoto R, et al. Bio-3D printing iPSC-derived human chondrocytes for articular cartilage regeneration. Biofabrication. 2021;13(4):044103. doi: 10.1088/1758-5090/ac1c99
   Web of Science ®Google Scholar
• Karageorgiou V, Kaplan D. Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials. 2005;26(27):5474–5491. doi: 10.1016/j.biomaterials.2005.02.002
   PubMed Web of Science ®Google Scholar
• Hu X, Wang Y, Tan Y, et al. A difunctional regeneration scaffold for knee repair based on aptamer-directed cell recruitment. Adv Mater. 2017;29(15): doi: 10.1002/adma.201605235
   Web of Science ®Google Scholar
• Shim JH, Jang KM, Hahn SK, et al. Three-dimensional bioprinting of multilayered constructs containing human mesenchymal stromal cells for osteochondral tissue regeneration in the rabbit knee joint. Biofabrication. 2016;8(1):014102. doi: 10.1088/1758-5090/8/1/014102
   PubMed Web of Science ®Google Scholar
• Wu X, Zhou M, Jiang F, et al. Marginal sealing around integral bilayer scaffolds for repairing osteochondral defects based on photocurable silk hydrogels. Bioact Mater. 2021;6(11):3976–3986. doi: 10.1016/j.bioactmat.2021.04.005
   PubMed Web of Science ®Google Scholar
• Xu X, Xu L, Wen C, et al. Programming assembly of biomimetic exosomes: an emerging theranostic nanomedicine platform. Mater Today Bio. 2023;22:100760. doi: 10.1016/j.mtbio.2023.100760
   PubMedGoogle Scholar
• Liang Y, Xu X, Li X, et al. Chondrocyte-targeted MicroRNA delivery by engineered exosomes toward a cell-free osteoarthritis therapy. ACS Appl Mater Inter. 2020;12(33):36938–36947. doi: 10.1021/acsami.0c10458
   PubMed Web of Science ®Google Scholar
• Liang Y, Duan L, Lu J, et al. Engineering exosomes for targeted drug delivery. Theranostics. 2021;11(7):3183–3195. doi: 10.7150/thno.52570
   PubMed Web of Science ®Google Scholar
• Duan L, Xu L, Xu X, et al. Exosome-mediated delivery of gene vectors for gene therapy. Nanoscale. 2021;13(3):1387–1397. doi: 10.1039/D0NR07622H
   PubMed Web of Science ®Google Scholar
• Liu Q, Li D, Pan X, et al. Targeted therapy using engineered extracellular vesicles: principles and strategies for membrane modification. J Nanobiotechnol. 2023;21(1):334. doi: 10.1186/s12951-023-02081-0
   PubMed Web of Science ®Google Scholar
• Iqbal Z, Rehman K, Xia J, et al. Biomaterial-assisted targeted and controlled delivery of CRISPR/Cas9 for precise gene editing. Biomater Sci. 2023;11(11):3762–3783. doi: 10.1039/D2BM01636B
   PubMed Web of Science ®Google Scholar
• Liang Y, Iqbal Z, Wang J, et al. Cell-derived extracellular vesicles for CRISPR/Cas9 delivery: engineering strategies for cargo packaging and loading. Biomater Sci. 2022;10(15):4095–4106. doi: 10.1039/D2BM00480A
   PubMed Web of Science ®Google Scholar
• Huang J, Xiong J, Yang L, et al. Cell-free exosome-laden scaffolds for tissue repair. Nanoscale. 2021;13(19):8740–8750. doi: 10.1039/D1NR01314A
   PubMed Web of Science ®Google Scholar
• Xu L, Xu X, Liang Y, et al. Osteoclast-targeted delivery of anti-miRNA oligonucleotides by red blood cell extracellular vesicles. J Control Release. 2023;358:259–272. doi: 10.1016/j.jconrel.2023.04.043
   PubMed Web of Science ®Google Scholar
• Anandhapadman A, Venkateswaran A, Jayaraman H, et al. Advances in 3D printing of composite scaffolds for the repairment of bone tissue associated defects. Biotechnol Prog. 2022;38(3):e3234. doi: 10.1002/btpr.3234
   PubMed Web of Science ®Google Scholar
• You D, Chen G, Liu C, et al. 4D printing of multi-responsive membrane for accelerated in vivo bone healing via remote regulation of stem cell fate. Adv Funct Mater. 2021;31(40):2103920. doi: 10.1002/adfm.202103920
   Web of Science ®Google Scholar