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Determining the affinities of high-affinity antibodies using KinExA and surface plasmon resonance

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Article: 2291209 | Received 26 Oct 2023, Accepted 30 Nov 2023, Published online: 13 Dec 2023

References

  • Martin KP, Grimaldi C, Grempler R, Hansel S, Kumar S. Trends in industrialization of biotherapeutics: a survey of product characteristics of 89 antibody-based biotherapeutics. MAbs. 2023;15(1):2191301. doi:10.1080/19420862.2023.2191301.
  • Kaplon H, Reichert JM. Antibodies to watch in 2019. MAbs. 2019;11(2):219–8. doi:10.1080/19420862.2018.1556465.
  • Jain T, Sun T, Durand S, Hall A, Houston NR, Nett JH, Sharkey B, Bobrowicz B, Caffry I, Yu Y, et al. Biophysical properties of the clinical-stage antibody landscape. Proc Natl Acad Sci U S A. 2017;114(5):944–949. doi:10.1073/pnas.1616408114.
  • Azevedo Reis Teixeira A, Erasmus MF, D’Angelo S, Naranjo L, Ferrara F, Leal-Lopes C, Durrant O, Galmiche C, Morelli A, Scott-Tucker A, et al. Drug-like antibodies with high affinity, diversity and developability directly from next-generation antibody libraries. MAbs. 2021;13(1):1980942. doi:10.1080/19420862.2021.1980942.
  • Ferrara F, Erasmus MF, D’Angelo S, Leal-Lopes C, Teixeira AA, Choudhary A, Honnen W, Calianese D, Huang D, Peng L, et al. A pandemic-enabled comparison of discovery platforms demonstrates a naïve antibody library can match the best immune-sourced antibodies. Nat Commun. 2022;13(1):462. doi:10.1038/s41467-021-27799-z.
  • Erasmus MF, Ferrara F, D’Angelo S, Spector L, Leal-Lopes C, Teixeira AA, Sørensen J, Nagpal S, Perea-Schmittle K, Choudhary A, et al. Insights into next generation sequencing guided antibody selection strategies. Sci Rep. 2023;13(1):18370. doi:10.1038/s41598-023-45538-w.
  • Oren R, Hod-Marco M, Haus-Cohen M, Thomas S, Blat D, Duvshani N, Denkberg G, Elbaz Y, Benchetrit F, Eshhar Z, et al. Functional comparison of engineered T cells carrying a native TCR versus TCR-like antibody–based chimeric antigen receptors indicates affinity/avidity thresholds. J Immunol. 2014;193(11):5733–5743. doi:10.4049/jimmunol.1301769.
  • Mao R, Kong W, He Y. The affinity of antigen-binding domain on the antitumor efficacy of CAR T cells: moderate is better. Front Immunol. 2022;13:1032403. doi:10.3389/fimmu.2022.1032403.
  • Yu X, Orr CM, Chan HTC, James S, Penfold CA, Kim J, Inzhelevskaya T, Mockridge CI, Cox KL, Essex JW, et al. Reducing affinity as a strategy to boost immunomodulatory antibody agonism. Nature. 2023;614(7948):539–547. doi:10.1038/s41586-022-05673-2.
  • Myszka DG. Improving biosensor analysis. J Mol Recognit. 1999;12(5):279–284. doi:10.1002/(SICI)1099-1352(199909/10)12:5<279:AID-JMR473>3.0.CO;2-3.
  • Bertoglio F, Meier D, Langreder N, Steinke S, Rand U, Simonelli L, Heine PA, Ballmann R, Schneider K-T, Roth KDR, et al. SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface. Nat Commun. 2021;12(1):1577. doi:10.1038/s41467-021-21609-2.
  • Knowling S, Clark J, Sjuts H, Abdiche YN. Direct comparison of label-free biosensor binding kinetics obtained on the biacore 8K and the Carterra LSA. SLAS Discov. 2020;25(9):977–984. doi:10.1177/2472555220934814.
  • Brown ME, Bedinger D, Lilov A, Rathanaswami P, Vásquez M, Durand S, Wallace-Moyer I, Zhong L, Nett JH, Burnina I, et al. Assessing the binding properties of the anti-PD-1 antibody landscape using label-free biosensors. PLoS ONE. 2020;15(3):e0229206. doi:10.1371/journal.pone.0229206.
  • Darling RJ, Brault PA. Kinetic exclusion assay technology: characterization of molecular interactions. Assay Drug Dev Technol. 2004;2(6):647–657. doi:10.1089/adt.2004.2.647.
  • Bee C, Abdiche YN, Stone DM, Collier S, Lindquist KC, Pinkerton AC, Pons J, Rajpal A. Exploring the dynamic range of the kinetic exclusion assay in characterizing antigen-antibody interactions. PLoS ONE. 2012;7(4):e36261. doi:10.1371/journal.pone.0036261.
  • Teixeira AAR, Erasmus MF, D’Angelo S, Naranjo L, Ferrara F, Leal-Lopes C, Durrant O, Galmiche C, Morelli A, Scott-Tucker A, et al. Drug-like antibodies with high affinity, diversity and developability directly from next-generation antibody libraries. MAbs. 2021;13(1):1980942. doi:10.1080/19420862.2021.1980942.