Extracellular vesicles in tumorigenesis, metastasis, chemotherapy resistance and intercellular communication in osteosarcoma

References

• Luetke A, Meyers PA, Lewis I, et al. Osteosarcoma treatment – Where do we stand? A state of the art review. Cancer Treat Rev. 2014;40(4):523–532. DOI:10.1016/j.ctrv.2013.11.006
   PubMed Web of Science ®Google Scholar
• Chen C, Xie L, Ren T, et al. Immunotherapy for osteosarcoma: fundamental mechanism, rationale, and recent breakthroughs. Cancer Lett. 2021;500:1–10.
   PubMed Web of Science ®Google Scholar
• Belayneh R, Fourman MS, Bhogal S, et al. Update on Osteosarcoma. Curr Oncol Rep. 2021;23(6):71. DOI:10.1007/s11912-021-01053-7
   PubMed Web of Science ®Google Scholar
• Tang Z, Lu Y, Chen Y, et al. Research progress Of MicroRNA in chemotherapy resistance of osteosarcoma. Technol Cancer Res Treat. 2021;20:15330338211034262.
   Web of Science ®Google Scholar
• Berhe S, Danzer E, Meyers PA, et al. Unusual abdominal metastases in osteosarcoma. J Pediatr Surg Case Rep. 2018;28:13–16.
   PubMed Web of Science ®Google Scholar
• Wei H, Chen J, Wang S, et al. A Nanodrug Consisting of Doxorubicin and Exosome Derived from Mesenchymal Stem Cells for Osteosarcoma Treatment in Vitro. 2019;14:8603–8610.
   Google Scholar
• Sole A, Grossetête S, Heintzé M, et al. Unraveling Ewing sarcoma tumorigenesis originating from patient-derived mesenchymal stem cells. Cancer Res. 2021;81(19):4994–5006. DOI:10.1158/0008-5472.CAN-20-3837
   PubMed Web of Science ®Google Scholar
• Mashouri L, Yousefi H, Aref AR, et al. Exosomes: composition, biogenesis, and mechanisms in cancer metastasis and drug resistance. Mol Cancer. 2019;18(1):75. DOI:10.1186/s12943-019-0991-5
   PubMed Web of Science ®Google Scholar
• Xiao Y, Yu D. Tumor microenvironment as a therapeutic target in cancer. Pharmacol Ther. 2021;221:107753.
   PubMed Web of Science ®Google Scholar
• Yang C, Tian Y, Zhao F, et al. Bone microenvironment and osteosarcoma metastasis. Int J Mol Sci. 2020;21(19):6985. DOI:10.3390/ijms21196985
   PubMed Web of Science ®Google Scholar
• Kok VC, Yu CC. Cancer-derived exosomes: their role in cancer biology and biomarker development. int J Nanomedicine. 2020;15:8019–8036.
   PubMed Web of Science ®Google Scholar
• Meng W, He C, Hao Y, et al. Prospects and challenges of extracellular vesicle-based drug delivery system: considering cell source. Drug Deliv. 2020;27(1):585–598. DOI:10.1080/10717544.2020.1748758
   PubMed Web of Science ®Google Scholar
• Gorgens A, Bremer M, Ferrer-Tur R, et al. Optimisation of imaging flow cytometry for the analysis of single extracellular vesicles by using fluorescence-tagged vesicles as biological reference material. J Extracell Vesicles. 2019;8(1):1587567. DOI:10.1080/20013078.2019.1587567
   PubMed Web of Science ®Google Scholar
• Huang T, Song C, Zheng L, et al. The roles of extracellular vesicles in gastric cancer development, microenvironment, anti-cancer drug resistance, and therapy. Mol Cancer. 2019;18(1):62. DOI:10.1186/s12943-019-0967-5
   PubMedGoogle Scholar
• Baietti MF, Zhang Z, Mortier E, et al. Syndecan–syntenin–alix regulates the biogenesis of exosomes. Nat Cell Biol. 2012;14(7):677–685. DOI:10.1038/ncb2502
   PubMed Web of Science ®Google Scholar
• Mohan A, Agarwal S, Clauss M, et al. Extracellular vesicles: novel communicators in lung diseases. Respir Res. 2020;21(1):175. DOI:10.1186/s12931-020-01423-y
   PubMed Web of Science ®Google Scholar
• Zhu S, Li S, Yi M, et al. Roles of microvesicles in tumor progression and clinical applications. int J Nanomedicine. 2021;16:7071–7090.
   PubMed Web of Science ®Google Scholar
• Jeppesen DK, Fenix AM, Franklin JL, et al. Reassessment of exosome composition. Cell. 2019;177(2):428–445 e18. DOI:10.1016/j.cell.2019.02.029
   PubMed Web of Science ®Google Scholar
• Sil S, Dagur RS, Liao K, et al. Strategies for the use of extracellular vesicles for the delivery of therapeutics. J Neuroimmune Pharmacol. 2020;15(3):422–442. DOI:10.1007/s11481-019-09873-y
   PubMed Web of Science ®Google Scholar
• Schiera G, Di Liegro CM, Di Liegro I. Extracellular membrane vesicles as vehicles for brain cell-to-cell interactions in physiological as well as pathological conditions. BioMed Res Int. 2015;2015:152926.
   PubMed Web of Science ®Google Scholar
• Gilligan KE, Dwyer RM. Engineering exosomes for cancer therapy. Int J Mol Sci. 2017;18(6):1122.
   PubMed Web of Science ®Google Scholar
• Katsuda T, Kosaka N, Ochiya T. The roles of extracellular vesicles in cancer biology: toward the development of novel cancer biomarkers. Proteomics. 2014;14(4–5):412–425.
   PubMed Web of Science ®Google Scholar
• Walker S, et al. Extracellular vesicle-based drug delivery systems for cancer treatment. Theranostics. 2019;9(26):8001–8017. DOI:10.7150/thno.37097
   PubMed Web of Science ®Google Scholar
• Zhang G, Huang X, Xiu H, et al. Extracellular vesicles: natural liver-accumulating drug delivery vehicles for the treatment of liver diseases. J Extracell Vesicles. 2020;10(2):e12030. DOI:10.1002/jev2.12030
   PubMed Web of Science ®Google Scholar
• Pan Y, Lin Y, Mi C. Cisplatin-resistant osteosarcoma cell-derived exosomes confer cisplatin resistance to recipient cells in an exosomal circ_103801-dependent manner. Cell Biol Int. 2021;45(4):858–868.
   PubMed Web of Science ®Google Scholar
• Li Q, Wang X, Jiang N, et al. Exosome-transmitted linc00852 associated with receptor tyrosine kinase AXL dysregulates the proliferation and invasion of osteosarcoma. Cancer Med. 2020;9(17):6354–6366. DOI:10.1002/cam4.3303
   PubMed Web of Science ®Google Scholar
• Han F, Pu P, Wang C, et al. Osteosarcoma cell-derived exosomal Mir-1307 promotes tumorgenesis via targeting AGAP1. BioMed Res Int. 2021;2021:7358153.
   PubMed Web of Science ®Google Scholar
• Sha L, Ma D, Chen C. Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/beta-catenin signal pathway. Aging. 2020;12(23):23598–23608. DOI:10.18632/aging.103546
   PubMedGoogle Scholar
• Li Y, Lin S, Xie X, et al. Highly enriched exosomal lncRNA OIP5-AS1 regulates osteosarcoma tumor angiogenesis and autophagy through miR-153 and ATG5. Am J Transl Res. 2021;13(5):4211–4223.
   PubMed Web of Science ®Google Scholar
• Wang J, Zhang H, Sun X, et al. Exosomal PD-L1 and N-cadherin predict pulmonary metastasis progression for osteosarcoma patients. J Nanobiotechnology. 2020;18(1):151. DOI:10.1186/s12951-020-00710-6
   PubMed Web of Science ®Google Scholar
• Hu X, Wen Y, Tan L-Y, et al. Exosomal long non-coding RNA ANCR Mediates Drug Resistance in Osteosarcoma. Front Oncol. 2021;11:735254.
   PubMed Web of Science ®Google Scholar
• Gong L, Bao Q, Hu C, et al. Exosomal miR-675 from metastatic osteosarcoma promotes cell migration and invasion by targeting CALN1. Biochem Biophys Res Commun. 2018;500(2):170–176. DOI:10.1016/j.bbrc.2018.04.016
   PubMed Web of Science ®Google Scholar
• Mannerstrom B, et al. Epigenetic alterations in mesenchymal stem cells by osteosarcoma-derived extracellular vesicles. Epigenetics. 2019;14(4):352–364. DOI:10.1080/15592294.2019.1585177
   PubMed Web of Science ®Google Scholar
• Baglio SR, Lagerweij T, Pérez-Lanzón M, et al. Blocking Tumor-Educated MSC Paracrine Activity Halts Osteosarcoma Progression. Clin Cancer Res. 2017;23(14):3721–3733. DOI:10.1158/1078-0432.CCR-16-2726
   PubMed Web of Science ®Google Scholar
• Zhong L, Liao D, Li J, et al. Rab22a-NeoF1 fusion protein promotes osteosarcoma lung metastasis through its secretion into exosomes. Signal Transduct Target Ther. 2021;6(1):59. DOI:10.1038/s41392-020-00414-1
   PubMed Web of Science ®Google Scholar
• Wolf-Dennen K, Gordon N, Kleinerman ES. Exosomal communication by metastatic osteosarcoma cells modulates alveolar macrophages to an M2 tumor-promoting phenotype and inhibits tumoricidal functions. Oncoimmunology. 2020;9(1):1747677.
   PubMed Web of Science ®Google Scholar
• Cheng Z, Wang L, Wu C, et al. Tumor-derived exosomes induced M2 Macrophage Polarization and Promoted the Metastasis of Osteosarcoma Cells Through Tim-3. Arch Med Res. 2021;52(2):200–210. DOI:10.1016/j.arcmed.2020.10.018
   PubMed Web of Science ®Google Scholar
• Zhang L, et al. Exosomes loaded with programmed death ligand-1 promote tumor growth by immunosuppression in osteosarcoma. Bioengineered. 2021;12(2):9520–9530. DOI:10.1080/21655979.2021.1996509
   PubMed Web of Science ®Google Scholar
• Lin L, Wang H, Guo W, et al. Osteosarcoma-derived exosomal miR-501-3p promotes osteoclastogenesis and aggravates bone loss. Cell Signal. 2021;82:109935.
   PubMed Web of Science ®Google Scholar
• Zhang Y, et al. H3K27 acetylation activated-COL6A1 promotes osteosarcoma lung metastasis by repressing STAT1 and activating pulmonary cancer-associated fibroblasts. Theranostics. 2021;11(3):1473–1492. DOI:10.7150/thno.51245
   PubMed Web of Science ®Google Scholar
• Zhang L, Cao H, Gu G, et al. Exosomal MiR-199a-5p Inhibits Tumorigenesis and Angiogenesis by Targeting VEGFA in Osteosarcoma. Front Oncol. 2022;12:884559.
   PubMed Web of Science ®Google Scholar
• Tao SC, Huang J-Y, Wei Z-Y, et al. EWSAT1 Acts in Concert with Exosomes in Osteosarcoma Progression and Tumor-Induced Angiogenesis: the “Double Stacking Effect”. Adv Biosyst. 2020;4(9):e2000152. DOI:10.1002/adbi.202000152
   PubMed Web of Science ®Google Scholar
• Raimondi L, et al. Osteosarcoma cell-derived exosomes affect tumor microenvironment by specific packaging of microRnas. Carcinogenesis. 2020;41(5):666–677. DOI:10.1093/carcin/bgz130
   PubMed Web of Science ®Google Scholar
• Yoshida A, Fujiwara T, Uotani K, et al. Clinical and Functional Significance of Intracellular and Extracellular microRNA-25-3p in Osteosarcoma. Acta Med Okayama. 2018;72(2):165–174. DOI:10.18926/AMO/55857
   PubMed Web of Science ®Google Scholar
• Mu X, et al. Notch Signaling Mediates Skeletal Muscle Atrophy in Cancer Cachexia Caused by Osteosarcoma. Sarcoma. 2016;2016:3758162.
   PubMedGoogle Scholar
• Zhao W, et al. Long non-coding RNA PVT1 encapsulated in bone marrow mesenchymal stem cell-derived exosomes promotes osteosarcoma growth and metastasis by stabilizing ERG and sponging miR-183-5p. Aging (Albany NY). 2019;11(21):9581–9596. DOI:10.18632/aging.102406
   PubMedGoogle Scholar
• Endo-Munoz L, et al. Progression of Osteosarcoma from a Non-Metastatic to a Metastatic Phenotype is Causally Associated with Activation of an Autocrine and Paracrine uPA Axis. PLoS ONE. 2015;10(8):e0133592. DOI:10.1371/journal.pone.0133592
   PubMed Web of Science ®Google Scholar
• Mazumdar A, Urdinez J, Boro A, et al. Exploring the Role of Osteosarcoma-Derived Extracellular Vesicles in Pre-Metastatic Niche Formation and Metastasis in the 143-B Xenograft Mouse Osteosarcoma Model. Cancers (Basel). 2020;12(11):3457. DOI:10.3390/cancers12113457
   PubMed Web of Science ®Google Scholar
• Lin Z, Xie X, Lu S, et al. Noncoding RNAs in osteosarcoma: implications for drug resistance. Cancer Lett. 2021;504:91–103.
   PubMed Web of Science ®Google Scholar
• Xiao X, Wang W, Li Y, et al. HSP90AA1-mediated autophagy promotes drug resistance in osteosarcoma. J Exp Clin Cancer Res. 2018;37(1):201. DOI:10.1186/s13046-018-0880-6
   PubMedGoogle Scholar
• Torreggiani E, Roncuzzi L, Perut F, et al. Multimodal transfer of MDR by exosomes in human osteosarcoma. Int J Oncol. 2016;49(1):189–196. DOI:10.3892/ijo.2016.3509
   PubMed Web of Science ®Google Scholar
• Zhan H, Xiao J, Wang P, et al. Exosomal CTCF Confers Cisplatin Resistance in Osteosarcoma by Promoting Autophagy via the IGF2-AS/miR-579-3p/msh6 Axis. J Oncol. 2022;2022:9390611.
   PubMed Web of Science ®Google Scholar
• Qin T, Zhu W, Kan X, et al. Luteolin attenuates the chemoresistance of osteosarcoma through inhibiting the PTN/β-catenin/mdr1 signaling axis by upregulating miR-384. J Bone Oncol. 2022;34:100429.
   PubMed Web of Science ®Google Scholar
• Yati S, Silathapanasakul A, Thakaeng C, et al. Extracellular Vesicle-Mediated IL-1 Signaling in Response to Doxorubicin Activates PD-L1 Expression in Osteosarcoma Models. Cells. 2022;11(6):1042. DOI:10.3390/cells11061042
   PubMed Web of Science ®Google Scholar
• Jerez S, Araya H, Thaler R, et al. Proteomic Analysis of Exosomes and Exosome-Free Conditioned Media from Human Osteosarcoma Cell Lines Reveals Secretion of Proteins Related to Tumor Progression. J Cell Biochem. 2017;118(2):351–360. DOI:10.1002/jcb.25642
   PubMed Web of Science ®Google Scholar
• Jerez S, Araya H, Hevia D, et al. Extracellular vesicles from osteosarcoma cell lines contain miRnas associated with cell adhesion and apoptosis. Gene. 2019;710:246–257. DOI:10.1016/j.gene.2019.06.005
   PubMed Web of Science ®Google Scholar
• Lin L, Huang K, Guo W, et al. Conditioned medium of the osteosarcoma cell line U2OS induces hBmscs to exhibit characteristics of carcinoma-associated fibroblasts via activation of IL-6/STAT3 signalling. J Biochem. 2020;168(3):265–271. DOI:10.1093/jb/mvaa044
   PubMed Web of Science ®Google Scholar
• Lagerweij T, Perez-Lanzon M, Baglio SR. A preclinical mouse model of osteosarcoma to define the extracellular vesicle-mediated communication between tumor and mesenchymal stem cells. J Vis Exp. 2018;2018(135). DOI:10.3791/56932-v
   Google Scholar
• Luo T, Zhou X, Jiang E, et al. Osteosarcoma NA-19a-3p. Front Oncol. 2021;11:618662.
   PubMed Web of Science ®Google Scholar
• Garimella R, Washington L, Isaacson J, et al. Extracellular membrane vesicles derived from 143b osteosarcoma cells contain pro-osteoclastogenic cargo: a novel communication mechanism in osteosarcoma bone microenvironment. Transl Oncol. 2014;7(3):331–340. DOI:10.1016/j.tranon.2014.04.011
   PubMed Web of Science ®Google Scholar
• Araki Y, Aiba H, Yoshida T, et al. Osteosarcoma-derived small extracellular vesicles enhance tumor metastasis and suppress osteoclastogenesis by miR-146a-5p. Front Oncol. 2021;11:667109.
   PubMed Web of Science ®Google Scholar
• Ucci A, Cappariello A, Ponzetti M, et al. Anti-osteoblastogenic,pro-inflammatory and pro-angiogenic effect of extracellular vesicles isolated from the human osteosarcoma cell line MNNG/HOS. Bone. 2021;153:116130. DOI:10.1016/j.bone.2021.116130
   PubMed Web of Science ®Google Scholar
• Urciuoli E, Giorda E, Scarsella M, et al. Osteosarcoma-derived extracellular vesicles induce a tumor-like phenotype in normal recipient cells. J Cell Physiol. 2018;233(8):6158–6172. DOI:10.1002/jcp.26464
   PubMed Web of Science ®Google Scholar
• Mazumdar A, Urdinez J, Boro A, et al. Osteosarcoma-derived extracellular vesicles induce lung fibroblast reprogramming. Int J Mol Sci. 2020;21(15):5451. DOI:10.3390/ijms21155451
   PubMed Web of Science ®Google Scholar
• Wang B, Wang X, Li P, et al. Osteosarcoma cell-derived exosomal ELFN1-AS1 mediates macrophage M2 polarization via sponging miR-138-5p and miR-1291 to promote the tumorgenesis of osteosarcoma. Front Oncol. 2022;12:881022.
   PubMed Web of Science ®Google Scholar
• Huang Y, Liu W, He B, et al. Exosomes derived from bone marrow mesenchymal stem cells promote osteosarcoma development by activating oncogenic autophagy. J Bone Oncol. 2020;21:100280.
   PubMed Web of Science ®Google Scholar
• Zhang H, Wang J, Ren T, et al. Bone marrow mesenchymal stem cell-derived exosomal miR-206 inhibits osteosarcoma progression by targeting TRA2B. Cancer Lett. 2020;490:54–65.
   PubMed Web of Science ®Google Scholar
• Lu Y, Cao G, Lan H, et al. Chondrocyte-derived exosomal miR-195 inhibits osteosarcoma cell proliferation and anti-apoptotic by targeting KIF4A in vitro and in vivo. Transl Oncol. 2022;16:101289.
   PubMed Web of Science ®Google Scholar
• Wang JW, Wu X-F, Gu X-J, et al. Exosomal miR-1228 from cancer-associated fibroblasts promotes cell migration and invasion of osteosarcoma by directly targeting SCAI. Oncol Res. 2019;27(9):979–986. DOI:10.3727/096504018X15336368805108
   PubMed Web of Science ®Google Scholar
• Zhao A, Zhao Z, Liu W, et al. Carcinoma-associated fibroblasts promote the proliferation and metastasis of osteosarcoma by transferring exosomal LncRNA SNHG17. Am J Transl Res. 2021;13(9):10094–10111.
   PubMed Web of Science ®Google Scholar
• Shimbo K, Miyaki S, Ishitobi H, et al. Exosome-formed synthetic microRNA-143 is transferred to osteosarcoma cells and inhibits their migration. Biochem Biophys Res Commun. 2014;445(2):381–387. DOI:10.1016/j.bbrc.2014.02.007
   PubMed Web of Science ®Google Scholar
• Xu Z, Zhou X, Wu J, et al. Mesenchymal stem cell-derived exosomes carrying microRNA-150 suppresses the proliferation and migration of osteosarcoma cells via targeting IGF2BP1. Transl Cancer Res. 2020;9(9):5323–5335. DOI:10.21037/tcr-20-83
   PubMed Web of Science ®Google Scholar
• Lin S, Zhu B, Huang G, et al. Microvesicles derived from human bone marrow mesenchymal stem cells promote U2OS cell growth under hypoxia: the role of PI3K/AKT and HIF-1α. Hum Cell. 2019;32(1):64–74. DOI:10.1007/s13577-018-0224-z
   PubMed Web of Science ®Google Scholar
• Zhou J, Xu L, Yang P, et al. The exosomal transfer of human bone marrow mesenchymal stem cell-derived miR-1913 inhibits osteosarcoma progression by targeting NRSN2. Am J Transl Res. 2021;13(9):10178–10192.
   PubMed Web of Science ®Google Scholar
• Liu W, Wang B, Duan A, et al. Exosomal transfer of miR-769-5p promotes osteosarcoma proliferation and metastasis by targeting DUSP16. Cancer Cell Int. 2021;21(1):541. DOI:10.1186/s12935-021-02257-4
   PubMed Web of Science ®Google Scholar
• Qin F, Tang H, Zhang Y, et al. Bone marrow-derived mesenchymal stem cell-derived exosomal microRNA-208a promotes osteosarcoma cell proliferation, migration, and invasion. J Cell Physiol. 2020;235(5):4734–4745. DOI:10.1002/jcp.29351
   PubMed Web of Science ®Google Scholar
• Qi J, Zhang R, Wang Y. Exosomal miR-21-5p derived from bone marrow mesenchymal stem cells promote osteosarcoma cell proliferation and invasion by targeting PIK3R1. J Cell Mol Med. 2021;25(23):11016–11030.
   PubMed Web of Science ®Google Scholar
• Wu C, Li Z, Feng G, et al. Tumor suppressing role of serum-derived exosomal microRNA-15a in osteosarcoma cells through the GATA binding protein 2/murine double minute 2 axis and the p53 signaling pathway. Bioengineered. 2021;12(1):8378–8395. DOI:10.1080/21655979.2021.1987092
   PubMed Web of Science ®Google Scholar
• Ge X, Liu W, Zhao W, et al. Exosomal TRAnsfer of LCP1 promotes osteosarcoma cell tumorigenesis and metastasis by activating the JAK2/STAT3 Signaling Pathway. Mol Ther Nucleic Acids. 2020;21:900–915.
   PubMedGoogle Scholar
• Qi J, Zhou Y, Jiao Z, et al. Exosomes derived from human bone marrow mesenchymal stem cells promote tumor growth through hedgehog signaling pathway. Cell Physiol Biochem. 2017;42(6):2242–2254. DOI:10.1159/000479998
   PubMedGoogle Scholar
• Feng D, Li Z, Yang L, et al. BMSC-EV-derived lncRNA NORAD facilitates migration, invasion, and angiogenesis in osteosarcoma cells by regulating CREBBP via Delivery of miR-877-3p. Oxid Med Cell Longev. 2022;2022:8825784.
   PubMed Web of Science ®Google Scholar
• Li F, Chen X, Shang C, et al. Bone marrow mesenchymal stem cells-derived extracellular vesicles promote proliferation, invasion and migration of osteosarcoma cells via the lncRNA MALAT1/miR-143/NRSN2/Wnt/β-Catenin Axis. Onco Targets Ther. 2021;14:737–749.
   PubMed Web of Science ®Google Scholar
• Shi Z, Wang K, Xing Y, et al. CircNRIP1 encapsulated by bone marrow mesenchymal stem cell–derived extracellular vesicles aggravates osteosarcoma by modulating the miR-532-3p/akt3/pi3k/akt Axis. Front Oncol. 2021;11:658139.
   PubMed Web of Science ®Google Scholar
• Wang Y, Chu Y, Li K, et al. Exosomes secreted by adipose-derived mesenchymal stem cells foster metastasis and osteosarcoma proliferation by increasing COLGALT2 Expression. Front Cell Dev Biol. 2020;8:353.
   PubMed Web of Science ®Google Scholar
• Zhang K, Dong C, Chen M, et al. Extracellular vesicle-mediated delivery of miR-101 inhibits lung metastasis in osteosarcoma. Theranostics. 2020;10(1):411–425. DOI:10.7150/thno.33482
   PubMed Web of Science ®Google Scholar
• Zhang H, Yu Y, Wang J, et al. Macrophages-derived exosomal lncRNA LIFR-AS1 promotes osteosarcoma cell progression via miR-29a/NFIA axis. Cancer Cell Int. 2021;21(1):192. DOI:10.1186/s12935-021-01893-0
   PubMed Web of Science ®Google Scholar
• Liu W, Long Q, Zhang W, et al. MiRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis. Aging (Albany NY. Aging. 2021;13(15):19760–19775. DOI:10.18632/aging.203388
   PubMedGoogle Scholar
• Wang L, Wu J, Song S, et al. Plasma exosome-derived sentrin sumo-specific protease 1: a prognostic biomarker in patients with osteosarcoma. Front Oncol. 2021;11:625109.
   PubMedGoogle Scholar
• Zhang H, Wang J, Ren T, et al. LncRNA CASC15 is upregulated in osteosarcoma plasma exosomes and CASC15 knockdown inhibits osteosarcoma progression by regulating miR-338-3p/rab14 Axis. onco Targets Ther. 2020;13:12055–12066.
   PubMed Web of Science ®Google Scholar
• Li S, Pei Y, Wang W, et al. Extracellular nanovesicles-transmitted circular RNA has_circ_0000190 suppresses osteosarcoma progression. J Cell Mol Med. 2020;24(3):2202–2214. DOI:10.1111/jcmm.14877
   PubMed Web of Science ®Google Scholar
• Strzelecka-Kiliszek A, Bozycki L, Mebarek S, et al. Characteristics of minerals in vesicles produced by human osteoblasts hFOB 1.19 and osteosarcoma Saos-2 cells stimulated for mineralization. J Inorg Biochem. 2017;171:100–107.
   PubMed Web of Science ®Google Scholar
• Bozycki L, Mroczek J, Bessueille L, et al. Annexins A2, A6 and fetuin-a affect the process of mineralization in vesicles derived from human osteoblastic hfob 1.19 and osteosarcoma saos-2 Cells. Int J Mol Sci. 2021;22(8):3993. DOI:10.3390/ijms22083993
   PubMed Web of Science ®Google Scholar
• Yang J, Hu Y, Wang L, et al. Human umbilical vein endothelial cells derived-exosomes promote osteosarcoma cell stemness by activating Notch signaling pathway. Bioengineered. 2021;12(2):11007–11017. DOI:10.1080/21655979.2021.2005220
   PubMed Web of Science ®Google Scholar
• Han Y, Guo W, Ren T, et al. Tumor-associated macrophages promote lung metastasis and induce epithelial-mesenchymal transition in osteosarcoma by activating the COX-2/STAT3 axis. Cancer Lett. 2019;440-441:116–125.
   PubMed Web of Science ®Google Scholar
• Vallabhaneni KC, Hassler MY, Abraham A, et al. Mesenchymal stem/stromal cells under stress increase osteosarcoma migration and apoptosis resistance via extracellular vesicle mediated communication. PLoS ONE. 2016;11(11):e0166027. DOI:10.1371/journal.pone.0166027
   PubMed Web of Science ®Google Scholar
• Keremu A, Aila P, Tusun A, et al. Extracellular vesicles from bone mesenchymal stem cells transport microRNA-206 into osteosarcoma cells and target NRSN2 to block the ERK1/2-Bcl-xL signaling pathway. Eur J Histochem. 2022;66(3).
   PubMed Web of Science ®Google Scholar
• Cambier L, Stachelek K, Triska M, et al. Extracellular vesicle-associated repetitive element DNAs as candidate osteosarcoma biomarkers. Sci Rep. 2021;11(1):94. DOI:10.1038/s41598-020-77398-z
   PubMed Web of Science ®Google Scholar
• Bao Q, Gong L, Wang J, et al. Extracellular Vesicle RNA Sequencing Reveals Dramatic Transcriptomic Alterations Between Metastatic and Primary Osteosarcoma in a Liquid Biopsy Approach. Ann Surg Oncol. 2018;25(9):2642–2651. DOI:10.1245/s10434-018-6642-z
   PubMed Web of Science ®Google Scholar
• Xu JF, Wang Y-P, Zhang S-J, et al. Exosomes containing differential expression of microRNA and mRNA in osteosarcoma that can predict response to chemotherapy. Oncotarget. 2017;8(44):75968–75978. DOI:10.18632/oncotarget.18373
   PubMedGoogle Scholar
• Ye Z, Zheng Z, Peng L. MicroRNA profiling of serum exosomes in patients with osteosarcoma by high-throughput sequencing. J Investig Med. 2020;68(4):893–901.
   PubMed Web of Science ®Google Scholar
• Cuscino N, Raimondi L, De Luca A, et al. Gathering novel circulating exosomal microRNA in osteosarcoma cell lines and possible implications for the disease. Cancers (Basel). 2019;11(12).
   PubMed Web of Science ®Google Scholar
• Agarwal P, Crepps MP, Stahr NA, et al. Identification of canine circulating miRNAs as tumor biospecific markers using next-generation sequencing and Q-RT-PCR. Biochem Biophys Rep. 2021;28:101106.
   PubMedGoogle Scholar
• Fujiwara T, Uotani K, Yoshida A, et al. Clinical significance of circulating miR-25-3p as a novel diagnostic and prognostic biomarker in osteosarcoma. Oncotarget. 2017;8(20):33375–33392. DOI:10.18632/oncotarget.16498
   PubMedGoogle Scholar
• Huo S, Dou D. Circ_0056285 regulates proliferation, apoptosis and glycolysis of osteosarcoma cells via mir-1244/trim44 axis. Cancer Manag Res. 2021;13:1257–1270.
   PubMed Web of Science ®Google Scholar
• Shan S, Li H-F, Yang X-Y, et al. Higher lncRNA CASC15 expression predicts poor prognosis and associates with tumor growth in cervical cancer. Eur Rev Med Pharmacol Sci. 2019;23(2):507–512. DOI:10.26355/eurrev_201901_16862
   PubMed Web of Science ®Google Scholar
• Han Z, Yi J, Yang Y, et al. SERS and MALDI-TOF MS based plasma exosome profiling for rapid detection of osteosarcoma. Analyst. 2021;146(21):6496–6505. DOI:10.1039/D1AN01163D
   PubMed Web of Science ®Google Scholar
• Han Z, Peng C, Yi J, et al. Matrix-assisted laser desorption ionization mass spectrometry profiling of plasma exosomes evaluates osteosarcoma metastasis. iScience. 2021;24(8):102906. DOI:10.1016/j.isci.2021.102906
   PubMed Web of Science ®Google Scholar
• Weinman MA, Ramsey SA, Leeper HJ, et al. Exosomal proteomic signatures correlate with drug resistance and carboplatin treatment outcome in a spontaneous model of canine osteosarcoma. Cancer Cell Int. 2021;21(1):245. DOI:10.1186/s12935-021-01943-7
   PubMed Web of Science ®Google Scholar
• Brady JV, Troyer RM, Ramsey SA, et al. A preliminary proteomic investigation of circulating exosomes and discovery of biomarkers associated with the progression of osteosarcoma in a clinical model of spontaneous disease. Transl Oncol. 2018;11(5):1137–1146. DOI:10.1016/j.tranon.2018.07.004
   PubMed Web of Science ®Google Scholar
• Luu AK, Cadieux M, Wong M, et al. proteomic assessment of extracellular vesicles from canine tissue explants as a pipeline to identify molecular targets in osteosarcoma: pSMD14/Rpn11 as a proof of principle. Int J Mol Sci. 2022;23(6):3256. DOI:10.3390/ijms23063256
   PubMed Web of Science ®Google Scholar
• Xue Q, Yang Y, Yang L, et al. MiR-371b-5p-engineered exosomes enhances tumor inhibitory effect. Front Cell Dev Biol. 2021;9:750171.
   PubMed Web of Science ®Google Scholar
• Huang X, Wu W, Jing D, et al. Engineered exosome as targeted lncRNA MEG3 delivery vehicles for osteosarcoma therapy. J Control Release. 2022;343:107–117.
   PubMed Web of Science ®Google Scholar
• Notaro A, Emanuele S, Geraci F, et al. WIN55,212-2-induced Expression of Mir-29b1 favours the suppression of osteosarcoma cell migration in a SPARC-Independent Manner. Int J Mol Sci. 2019;20(20):5235. DOI:10.3390/ijms20205235
   PubMed Web of Science ®Google Scholar
• Wang BD, Yu X-J, Hou J-C, et al. Bevacizumab attenuates osteosarcoma angiogenesis by suppressing MIAT encapsulated by serum-derived extracellular vesicles and facilitating miR-613-mediated GPR158 inhibition. Cell Death Dis. 2022;13(3):272. DOI:10.1038/s41419-022-04620-3
   PubMed Web of Science ®Google Scholar