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Emerging Microbes & Infections Volume 12, 2023 - Issue 1
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Research Article

Respiratory microbiota imbalance in children with Mycoplasma pneumoniae pneumonia

Yacui Wanga Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;b Department of Interventional Pulmonology, China Laboratory of Respiratory Infection Diseases, Beijing Children’s Hospital, Capital Medical University, Beijing, People’s Republic of China;c Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing, People’s Republic of ChinaView further author information
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Xiaoran Yua Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;d Department of Center for Non-communicable Disease Management, Beijing Children's Hospital, Capital Medical University, Beijing, People’s Republic of ChinaView further author information
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Fang Liua Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;b Department of Interventional Pulmonology, China Laboratory of Respiratory Infection Diseases, Beijing Children’s Hospital, Capital Medical University, Beijing, People’s Republic of ChinaView further author information
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Xue Tiana Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;c Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing, People’s Republic of ChinaView further author information
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Shuting Quana Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;c Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing, People’s Republic of ChinaView further author information
,
Anxia Jiaoa Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;b Department of Interventional Pulmonology, China Laboratory of Respiratory Infection Diseases, Beijing Children’s Hospital, Capital Medical University, Beijing, People’s Republic of ChinaView further author information
,
Xuemei Yanga Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;c Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing, People’s Republic of ChinaView further author information
,
Xi Zenga Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;c Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing, People’s Republic of ChinaView further author information
,
Weiwei Jiaoa Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;c Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing, People’s Republic of ChinaView further author information
,
Hui Qia Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;c Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing, People’s Republic of ChinaView further author information
,
Fang Xua Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;c Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing, People’s Republic of ChinaView further author information
,
Qinjing Lia Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;e Department of Infectious Diseases, Beijing Children’s Hospital, Capital Medical University, Beijing, People’s Republic of ChinaView further author information
,
Shuping Liua Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;e Department of Infectious Diseases, Beijing Children’s Hospital, Capital Medical University, Beijing, People’s Republic of ChinaView further author information
,
Baoping Xua Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;f Department of Respiratory Diseases, Beijing Children’s Hospital, Capital Medical University, Beijing, People’s Republic of ChinaCorrespondence[email protected]
View further author information
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Lin Suna Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;c Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing, People’s Republic of ChinaCorrespondence[email protected]
View further author information
&
Adong Shena Beijing Children’s Hospital, Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Center for Children’s Health, Beijing, People’s Republic of China;c Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing, People’s Republic of China;g Beijing Children's Hospital Baoding Hospital, Capital Medical University, Baoding, People’s Republic of ChinaCorrespondence[email protected]
View further author information
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Article: 2202272 | Received 20 Nov 2022, Accepted 07 Apr 2023, Published online: 09 May 2023

References

  • Li ZJ, Zhang HY, Ren LL, et al. Etiological and epidemiological features of acute respiratory infections in China. Nat Commun. 2021 Aug 18;12(1):5026.
  • Gao LW, Yin J, Hu YH, et al. The epidemiology of paediatric Mycoplasma pneumoniae pneumonia in North China: 2006 to 2016. Epidemiol Infect. 2019 Jan;147:e192.
  • Kutty PK, Jain S, Taylor TH, et al. Mycoplasma pneumoniae Among Children Hospitalized With Community-acquired Pneumonia. Clin Infect Dis. 2019 Jan 1;68(1):5–12.
  • Waites KB, Talkington DF. Mycoplasma pneumoniae and its role as a human pathogen. Clin Microbiol Rev. 2004 Oct;17(4):697–728. table of contents.
  • Wu BG, Segal LN. The lung microbiome and its role in pneumonia. Clin Chest Med. 2018 Dec;39(4):677–689.
  • Esposito S, Principi N. Impact of nasopharyngeal microbiota on the development of respiratory tract diseases. Eur J Clin Microbiol Infect Dis. 2018 Jan;37(1):1–7.
  • Budden KF, Shukla SD, Rehman SF, et al. Functional effects of the microbiota in chronic respiratory disease. Lancet Respir Med. 2019 Oct;7(10):907–920.
  • Dai W, Wang H, Zhou Q, et al. The concordance between upper and lower respiratory microbiota in children with Mycoplasma pneumoniae pneumonia. Emerg Microbes Infect. 2018 May 23;7(1):92.
  • Zhou Q, Xie G, Liu Y, et al. Different nasopharynx and oropharynx microbiota imbalance in children with Mycoplasma pneumoniae or influenza virus infection. Microb Pathog. 2020 Jul;144:104189.
  • Loens K, Ieven M. Mycoplasma pneumoniae: current knowledge on nucleic acid amplification techniques and serological diagnostics. Front Microbiol. 2016;7:448.
  • Li J, Sun L, Wu X, et al. Early diagnosis of Mycoplasma pneumoniae in children: simultaneous amplification and testing (SAT) is the key. Front Pediatr. 2019;7:441.
  • Räty R, Rönkkö E, Kleemola M. Sample type is crucial to the diagnosis of Mycoplasma pneumoniae pneumonia by PCR. J Med Microbiol. 2005 Mar;54(Pt 3):287-291.
  • Kelly BJ, Imai I, Bittinger K, et al. Composition and dynamics of the respiratory tract microbiome in intubated patients. Microbiome. 2016 Feb 11;4:7.
  • Harris M, Clark J, Coote N, et al. British Thoracic Society guidelines for the management of community acquired pneumonia in children: update 2011. Thorax. 2011 Oct;66(Suppl 2):ii1–23.
  • Subspecialty Group of Respiratory Diseases. The society of pediatrics, C.M.A.E.B., Chinese journal of pediatrics., guidelines for management of community acquired pneumonia in children (the revised edition of 2013) (I) [in Chinese]. Zhonghua Er Ke Za Zhi. 2013;51:745–752.
  • Li M, Chen Y, Li H, et al. Serum CXCL10/IP-10 may be a potential biomarker for severe Mycoplasma pneumoniae pneumonia in children. BMC Infect Dis. 2021 Sep 4;21(1):909.
  • Magoč T, Salzberg SL. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics. 2011 Nov 1;27(21):2957–2963.
  • Bokulich NA, Subramanian S, Faith JJ, et al. Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing. Nat Methods. 2013 Jan;10(1):57–59.
  • Quast C, Pruesse E, Yilmaz P, et al. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res. 2013 Jan;41(Database issue):D590–596.
  • Edgar RC, Haas BJ, Clemente JC, et al. UCHIME improves sensitivity and speed of chimera detection. Bioinformatics. 2011 Aug 15;27(16):2194–2200.
  • Edgar RC. UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nat Methods. 2013 Oct;10(10):996–998.
  • Lu Z, Dai W, Liu Y, et al. The alteration of nasopharyngeal and oropharyngeal microbiota in children with MPP and Non-MPP. Genes (Basel). 2017 Dec 11; 8(12):380
  • Whiteside SA, McGinniss JE, Collman RG. The lung microbiome: progress and promise. J Clin Invest. 2021 Aug 2;131(15):e150473.
  • Charlson ES, Bittinger K, Haas AR, et al. Topographical continuity of bacterial populations in the healthy human respiratory tract. Am J Respir Crit Care Med. 2011 Oct 15;184(8):957–963.
  • Wang H, Zhou Q, Dai W, et al. Lung microbiota and pulmonary inflammatory cytokines expression vary in children with tracheomalacia and adenoviral or Mycoplasma pneumoniae pneumonia. Front Pediatr. 2019;7:265.
  • Dickson RP, Erb-Downward JR, Freeman CM, et al. Spatial variation in the healthy human lung microbiome and the adapted island model of lung biogeography. Ann Am Thorac Soc. 2015 Jun;12(6):821–830.
  • Hao Y, Kuang Z, Jing J, et al. Mycoplasma pneumoniae modulates STAT3-STAT6/EGFR-FOXA2 signaling to induce overexpression of airway mucins. Infect Immun. 2014 Dec;82(12):5246–5255.
  • Medina JL, Coalson JJ, Brooks EG, et al. Mycoplasma pneumoniae CARDS toxin induces pulmonary eosinophilic and lymphocytic inflammation. Am J Respir Cell Mol Biol. 2012 Jun;46(6):815–822.
  • Bosch A, de Steenhuijsen Piters WAA, van Houten MA, et al. Maturation of the Infant respiratory microbiota, environmental drivers, and health consequences. a prospective cohort study. Am J Respir Crit Care Med. 2017 Dec 15;196(12):1582–1590.
  • Waites KB, Balish MF, Atkinson TP. New insights into the pathogenesis and detection of Mycoplasma pneumoniae infections. Future Microbiol. 2008 Dec;3(6):635–648.
  • Shimizu T. Inflammation-inducing Factors of Mycoplasma pneumoniae. Front Microbiol. 2016;7:414.
  • Naghib M, Hatam-Jahromi M, Niktab M, et al. Mycoplasma pneumoniae and toll-like receptors: A mutual avenue. Allergol Immunopathol (Madr). 2018 Sep-Oct;46(5):508–513.
  • He J, Liu M, Ye Z, et al. Insights into the pathogenesis of Mycoplasma pneumoniae (Review). Mol Med Rep. 2016 Nov;14(5):4030–4036.
  • Song Q, Xu BP, Shen KL. Bacterial co-infection in hospitalized children with Mycoplasma pneumoniae pneumonia. Indian Pediatr. 2016 Oct 8;53(10):879–882.
  • Welp AL, Bomberger JM. Bacterial community interactions during chronic respiratory disease. Front Cell Infect Microbiol. 2020;10:213.
  • Edin A, Granholm S, Koskiniemi S, et al. Development and laboratory evaluation of a real-time PCR assay for detecting viruses and bacteria of relevance for community-acquired pneumonia. J Mol Diagn. 2015 May;17(3):315–324.
  • Dicker AJ, Lonergan M, Keir HR, et al. The sputum microbiome and clinical outcomes in patients with bronchiectasis: a prospective observational study. Lancet Respir Med. 2021 Aug;9(8):885–896.
  • Rozas M, Brillet F, Callewaert C, et al. MinION™ nanopore sequencing of skin microbiome 16S and 16S-23S rRNA gene amplicons. Front Cell Infect Microbiol. 2021;11:806476.
  • Sulaiman I, Wu BG, Li Y, et al. Functional lower airways genomic profiling of the microbiome to capture active microbial metabolism. Eur Respir J. 2021 Jul 29;58(1):2003434.

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