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Review

Advances in skin gene therapy

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Pages 2069-2083 | Published online: 24 Feb 2005

Bibliography

  • ODLAND G: Structure of the skin. In: Biochemistry andPhysiology of the Skin. Goldsmith L (Ed.), Oxford University Press, New York, USA (1983):3–63.
  • GRIFFITHS CE, TRANFAGLIA MS, KANG S: Prolongedocclusion in the treatment of psoriasis: A clinical and immunohistologic study. J. Am. Acad. Dermatol (1995) 32:618–622.
  • MILLER A: Human gene therapy comes of age. Nature (1992) 357:455–460.
  • MULLIGAN R: The basic science of gene therapy. Science (1993) 260:926–932.
  • MORGAN R, ANDERSON W: Human gene therapy. Ann. Rev. Biochem. (1993) 62:191–217.
  • VOGEL JC: Keratinocyte gene therapy. Arch. Derm. (1993) 129:1478–1483.
  • TAICHMAN L: Epithelial gene therapy. In: The Keratino-cyte Handbook. Leigh I, Lane E, Watt F (Eds.), University of Cambridge, New York, USA (1994):543–551.
  • GREENHALGH D, ROTHNAGEL J, ROOP D: Epidermis: an attractive target tissue for gene therapy. J. Invest. Dermatol. (1994) 103:63S–69S.
  • KHAVARI PA: Therapeutic gene delivery to the skin. Mol. Med. Today (1997) 3:533–538.
  • CHOATE K, MEDALIE D, MORGAN J, KHAVARI PA: Corrective gene transfer in the human skin disorder lamellar ichthyosis. Nature Med. (1996) 2:1263–1267.
  • ••Phenotypic correction of a genetic skin disease by retroviraltransduction of patient keratinocytes subsequently grafted onto immunocompromised mice.
  • VAILLY J, GAGNOUX-PALACIOS L, DELL'AMBRA E et al.: Corrective gene transfer of keratinocytes from patients with junctional epidermolysis bullosa restores assembly of hemidesmosomes in reconstructed epithelia. Gene Ther. (1998) 5:1322–1332.
  • SEITZ C, GIUDICE G, BALDING S, MARINKOVICH M,KHAVARI P: BP180 gene delivery in junctional epider-molysis bullosa. Gene Ther (1999) 6:42–47.
  • LUGER T, SCHVVARTZ T: Evidence for an epidermalcytokine network. J. Invest. Dermatol. (1990) 95:100S–104S.
  • TOMIC-CANIC M, KOMINE M, FREEDBERG I, BLUMEN-BERG M: Epidermal signal transduction and transcrip-tion factor activation in activated keratinocytes. Dermatol. Sci. (1998) 17:167–181.
  • GERRARD A, HUDSON D, BROWNLEE G, WATT F: Towards gene therapy for haemophilia B using primary human keratinocytes. Nature Genet. (1993) 3:180–183.
  • EMING S, MEDALIE D, TOMPKINS R, YARMUSH M, MORGAN J: Genetically modified human keratinocytes overexpressing PDGF-A enhance the performance of a composite skin graft. Hum. Gene Ther. (1998) 9:529–539.
  • JENSEN T, SULLIVAN D, MORGAN R et al.: Retrovirus-mediated gene transfer of ornithine-8-aminotransf-erase into keratinocytes from gyrate atrophy patients. Hum. Gene Ther. (1997) 8:2125–2132.
  • ALEXEV V, IGOUCHEVA O, DOMASHENKO A, COTSARELIS G, YOON K: Localized in vivo genotypic and phenotypic correction of the albino mutation in skin by RNA-DNA oligonucleotide. Nature Biotechnol. (2000) 18:43–47.
  • HIRSCHOWITZ E, LEONARD S, SONG W et al.: Adenovirus-mediated expression of melanoma antigen gp75 as immuno therapy for metastatic melanoma. Gene Ther. (1998) 5:975–983.
  • NABEL G, GORDON D, BISHOP D et al.: Immune response in human melanoma after transfer of an allogeneic class I major histocompatibility complex gene with DNA-liposome complexes. Proc. Natl. Acad. ScL USA (1996) 93:15388–15393.
  • WALKER PS, SCHARTON-KERSTEN T, ROWTON E et al:Genetic immunization with glycoprotein 63 cDNA results in a helper T cell Type 1 immune response and protection in a murine model of leishmaniasis. Hum. Gene Ther. (1998) 13:1899–1907.
  • ZITVOGEL L, COUDERC B, MAYORDOMO J, ROBBINS P,LOTZE M, STORKUS W: IL-12-engineered dendritic cells serve as effective tumor vaccine adjuvants in viva Ann. NY Acad. ScL (1996) 795:284–293.
  • BOULOC A, WALKER PS, GRIEVEL JC, VOGEL JC, KATZ SI: Immunization through dermal delivery of protein-encoding DNA: a role for migratory dendritic cells. Eur.J. Immunol. (1999) 29:446–454.
  • ••Intradermal DNA injection results in transfection of dendriticcells which are able to induce humoral and cellular immune responses.
  • BYRNE C, FUCHS E: Probing keratinocyte and differen- tiation specificity of the human K5 promoter in vitro and in transgenic mice. Mol. Cell. Biol. (1993) 13:3176–3190.
  • WANG X, ZINKEL S, POLONSKY K, FUCHS E: Transgenic studies with a keratin promoter-driven growth hormone transgene: prospects for gene therapy. Proc. Nati Acad. Sci. USA (1997) 94:219–226.
  • ••Using a keratin-specific promoter expression of the humangrowth hormone as a transgene can be directed to selected cells in the epidermis (i.e., basal layer keratinocytes). The transgene product was shown to be secreted as a biologi-cally active hormone into the bloodstream of transgenic mice as well as animals grafted with transgenic skin.
  • CARROLL J, ALBERS K, GARLICK J, HARRINGTON R, TAICHMAN L: Tissue- and stratum-specific expression of the human involucrin promoter in transgenic mice. Proc. Natl. Acad. ScL USA (1993) 90:10270–10274.
  • CLARKSON T: Regulated gene expression systems. Gene Ther. (2000) 7:120–125.
  • MILLER N, WHELAN J: Progress in transcriptionally targeted and reg-ulatable vectors for gene therapy. Hum. Gene Ther. (1997) 8:803–815.
  • BICKENBACH JR, ROOP DR: Transduction of apreselected population of human epidermal stem cells: consequences for gene therapy. Proc. Assoc. Am. Phys. (1999) 111:184–189.
  • WILLIAMS R, JOHNSTON S, RIEDY M, DEVIT M, MCELLIGOTT S, SANFORD J: Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles. Proc. NatI Acad. Sci. USA (1991) 88:2726–2730.
  • ••Description of biolistic gene transfer into mouse skinshowing for the first time that DNA can be successfully delivered to skin cells.
  • DOMASHENKO A, GUPTA S, COTSARELIS G: Efficient delivery of transgenes to human hair follicle progenitor cells using topical lip oplex . Nature Biotech. (2000) 18:420–423.
  • ALEXANDER M, AKHURST R: Liposome-mediated gene transfer and expression via the skin. Hum. Mol. Genet. (1995) 4:2279–2285.
  • FAN H, LIN Q, MORRISSEY G, KHAVARI PA: Immuniza-tion via hair follicles by topical application of naked DNA to normal skin. Nature Biotech. (1999) 17:870–872.
  • ROLS M, DELTEIL C, GOLZIO M, DUMOND P, CROS S, TEISSIE J: In vivo electrically mediated protein and gene transfer in murine melanoma. Nature Biotech. (1998) 16:168–171.
  • SAWAMURA D, INA S, ITAI K et al.: In vivo gene introduc-tion into keratinocytes using jet injection. Gene Ther. (1999) 6:1785–1787.
  • ERIKSSON E, YAO F, SVENSJ0 T et al.: In vivo gene transfer to skin and wound by microseeding. j Surg. Res. (1997) 78:85–91.
  • CIERNIK I, KRAYENBUHL B, CARBONE D: Puncture-mediated gene transfer to the skin. Hum. Gene Ther. (1996) 7:893–899.
  • HENGGE UR, WALKER PS, VOGEL JC: Expression of naked DNA in human, pig and mouse skin. J. Clin. Invest. (1996) 15:2911–2916.
  • ••Intradermal plasmid injection into human skin grafts showssubsequent transgene expression in human keratinocytes.
  • HENGGE UR, CHAN EF, FOSTER RA, WALKER PS, VOGEL JC: Cytokine gene expression in epidermis with biological effects following injection of naked DNA. Nature Genet. (1995) 10:161–166.
  • ••Direct injection of plasmid DNA into pig skin results intransient expression of a biologically active factor in the epidermis.
  • CHOATE K, KHAVARI PA: Direct cutaneous gene delivery in a human genetic skin disease. Hum. Gene Ther. (1997) 20:1659–1665.
  • HOTTIGER M, DAM T, NICKOLOFF B, JOHNSON TM, NABEL G: Liposome-mediated gene transfer into human basal cell carcinoma. Gene Ther. (1999) 6:1929–1935.
  • FALO LD: Targeting the skin for genetic immunization.Proc. Assoc. Am. Phys. (1998) 111:211-219. Ashley Publications Ltd. All rights reserved.Exp. Opin. Invest. Drugs (2000) 9(9)
  • CONDON C, WATKINS S, CELLUZZI C, THOMPSON K, FALO LD: DNA-based immunization by in vivotransfec-tion of dendritic cells. Nature Med. (1996) 2:1122–1228.
  • ••Intracutaneous DNA vaccination of dendritic cells results inantigen-specific protective tumour immunity mediated by cytotoxic T-lymphocytes.
  • RAZ E, CARSON D, PARKER S et al: Intradermal gene immunization: the possible role of DNA uptake in the induction of cellular immunity to viruses. Proc. Natl. Acad. Sci. USA (1994) 91 :9519–9523.
  • RAZE, TIGHE H, SATO Y et al.: Preferential induction ofa Th1 immune response and inhibition of specific IgE antibody formation by plasmid DNA immunization. Proc. Nati Acad. Sci. USA (1996) 93:5141–5145.
  • BOYLE J, SILVA A, BRADY J, LEW A: DNA immunization: induction of higher avidity antibody and effect of route on T cell cytotoxicity. Proc. Nati Acad. Sci. USA (1997) 94:14626–14631.
  • SATO Y, ROMAN M, TIGHE H et al.: Inimuno stimulatory DNA sequences necessary for effective intradermal gene immunization. Science (1996) 273:352–354.
  • WEINER G, LIU H-M, WOOLDRIDGE J, DAHLE C, KRIEG A:Immun o stimulatory oligo deo x ynucleo tides containing the CpG motif are effective as immune adjuvants in tumor antigen immunization. Proc. Natl. Acad. Sci. USA (1997) 94:10833–10837.
  • WALKER PS, SCHARTON-KERSTEN T, KRIEG A et al: Irnmunostimulatory oligodeoxynucleotides promote protective immunity and provide systemic therapy for leishmaniasis via IL-12 and IEN-7-dependent mechanisms. Proc. Natl. Acad. Sci. USA (1999) 96:6970–6975.
  • COLE-STRAUSS A, YOON K, XIANG Y et al.: Correction ofthe mutation responsible for sickle cell anemia by an RNA-DNA oligonucleo tide. Science (1996) 6:1386–1389.
  • KREN B, BANDYOPADHAY P, STEER C: In vivo site-directed mutagenesis of the Factor IX gene by chimeric RNA/DNA oligonucleotides. Nature Med. (1998) 4:285–290.
  • LU B, SCOTT G, GOLDSMITH L: Amodel for keratinocyte gene therapy: preclinical and therapeutic considera-tions. Proc. Assoc. Am. Phys. (1996) 108:165–171.
  • WANG C, QUEVEDO M, LANNUTTI B et al: In vivo gene therapy with interleukin-12 inhibits primary vascular tumor growth and induces apoptosis in a mouse model. J. Invest. Dermatol (1999) 112:775–781.
  • ANDREE C, SWAIN W, PAGE C et al.: In vivo transfer andexpression of a human epidermal growth factor gene accelerates wound repair. Proc. Natl. Acad. Sci. USA (1994) 91:12188–12192.
  • PERTMER T, EISENBRAUN M, MCCABE D, PRAYAGA S, FULLER D, HAYNES J: Gene gun-based nucleic acid immunization. Elicitation of humoral and cytotoxic T lymphocyte responses following epidermal delivery of nan ogr am quantities of DNA. Vaccine (1995) 13:1427–1430.
  • FELTQUATE D, HEANEY S, WEBSTER R, ROBINSON H: Different T helper cell types and antibody isotypes generated by saline and gene gun DNA immunization. Immunol. (1997) 158:2278–2284.
  • NIEMIEC S, LATTA J, RAMACHANDRAN C, WEINER N, ROESSLER B: Perifollicular transgenic expression of human interleukin-1 receptor antagonist protein following topical application of novel lip osome-plasmid DNA formulations in vivo. J. Pharm. Sci. (1997) 86:701–708.
  • ROBBINS P, TAHARA H, GHIVIZZANI S: Viral vectors for gene therapy. Trends Biotechnol. (1998) 16:35–40.
  • GÜNZBURG W, SALMONS B: Virus vector design in gene therapy. Mol. Med. Today (1995) 1:410–407.
  • LU B, FEDEROFF H, WANG Y, GOLDSMITH L, SCOTT G: Topical application of viral vectors for epidermal gene transfer. J. Invest. Dermatol. (1997) 108:803–808.
  • YANG Y, SU Q, WILSON J: Role of viral antigens in destructive cellular immune responses to adenovirus vector-transduced cells in mouse lungs. J. Virol (1996) 70:7209–7212.
  • HARVEY B, WORGALL S, ELY S, LEOPOLD P, CRYSTAL RG:Cellular immune responses of healthy individuals to intradermal administration of an E1-E3- adenovirus gene transfer vector. Hum. Gene Ther. (1999) 10:2823–2837.
  • TANG D, SHI Z, CURIEL D: Vaccination onto bare skin.Nature (1997) 388:729–730.
  • BONNEKOH B, GREENHALGH D, CHEN SH et al.: Ex vivoand in vivo adenovirus-mediated gene therapy strate-gies induce a systemic anti-tumor immune defense in the B16 melanoma model. J. Invest. Dermatol. (1998) 110:867–871.
  • MORRIS J, RAMSEY W, WILDNER O, MUSLOW H, AGUILAR-CORDOVA E, BLAESE MR: A Phase I study of intralesional administration of an adenovirus vector expressing the HSV-1 thymidine kinase gene (AdV.RSV-TK) in combination with escalating doses of ganciclovir in patients with cutaneous metastatic malignant melanoma. Hum. Gene Ther. (2000) 11:487–503.
  • MILLER A, MILLER D, GARCIA J, LYNCH C: Use ofretroviral vectors for gene transfer and expression. Methods Enzymol. (1993) 217:581–599.
  • VILE R, TUSZYNSKI A, CASTLEDEN S: Retroviral vectors.From laboratory tools to molecular medicine. Mol Biotechnol (1996) 5:139–158.
  • GHAZIZADEH S, HARRINGTON R, TAICHMAN LB: In vivo transduction of mouse epidermis with recombinant retroviral vectors: implications for cutaneous gene therapy. Gene Ther. (1999) 6:1267–1275.
  • ••Epidermal cells can be transduced in vivo by applying theretroviral vector directly to highly proliferative keratinocytes in a superficial skin wound. Ashley Publications Ltd. All rights reserved.Exp. Opin. Invest. Drugs (2000) 9(9)
  • KLIMATECHEVA E, ROSENBLATT J, PLANELLES V: Izntiviral vectors and gene therapy. Front. Biosci. (1999) 4:D481–496.
  • PONEC M: Reconstruction of human epidermis onde-epidermized dermis: expression of differentiation-specific protein markers and lipid composition. Toxicol. In vitro (1991) 5:597–606.
  • GARLICK J, TAICHMAN L: Effect of TGF-B1 onre-epithelialization of human keratinocytes in vitro: an organotypic model. J. Invest. Dermatol. (1994) 103:554–559.
  • COSSET F, TAKEUCHI Y, BATTINI JL, WEISS R, COLLINS M:High-titer packaging cells producing recombinant retroviruses resistant to human serum. J. Virol. (1995) 69:7430–7436.
  • KOLODKA TM, GARLICK JA, TAICHMAN LB: Evidence for Keratinocyte stem cells in VitrO: Long term engraft-ment and persistence of transgene expression from retrovirus-transduced keratinocytes. Proc. Nad Acad. Sci. USA (1998) 95:4356–4361.
  • ••Grafts of human keratinocytes transduced in vitro showtransgene expression for 40 weeks in vivo, consistent with successful gene transfer into keratinocyte stem cells.
  • FREIBERG R, CHOATE K, DENG H, ALPERIN E, SHAPIRO L,KHAVARI PA: A model of corrective gene transfer in X-linked ichthyosis. Hum. Mot. Genet. (1997) 6:933–937.
  • CARREAU M, QUILLIET X, EVENO E et al.: Functionalretroviral vector for gene therapy of xeroderma pigrnentosum group Dpatients. Hum. Gene Ther. (1995) 6:1307–1315.
  • ZENG L, SARASIN A, MEZZINA M: Retrovirus-mediatedDNA repair gene transfer into xeroderma pigrnen-tosum cells: perspectives for a gene therapy. Cell. Biol. Toxicol (1998) 14:105–110.
  • WHITE S, PAGE S, MARGARITIS P, BROWNLEE G: Long-term expression of human clotting Factor IX from retrovirally transduced primary human keratinocytes in vivo. Hum. Gene Ther. (1998) 9:1187–1195.
  • MORGAN J, BARRANDON Y, GREEN H, MULLIGAN R: Expression of an exogenous growth hormone gene by transplantable human epidermal cells. Science (1987) 237:1476–1479.
  • FENJVES E, SMITH J, ZARADIC S, TAICHMAN LB: Systemic delivery of secreted protein by grafts of epidermal keratinocytes: prospects for keratinocyte gene therapy. Hum. Gene Ther. (1994) 5:1241–1248.
  • KAFRI T, BLOMER U, PETERSON D, GAGE F, VERMA I: Sustained expression of genes delivered directly into liver and muscle by lentiviral vectors. Nature Genet. (1997) 17:314–317.
  • CHOATE K, KHAVARI PA: Sustain ability of keratinocytegene transfer and cell survival in viva Hum. Gene Ther. (1997) 8:895–901.
  • LEVY L, BROAD S, ZHU A et al.: Optimised retroviralinfection of human epidermal keratinocytes: long-term expression of transduced integrin gene following grafting on to SCID mice. Gene Ther. (1998) 5:913–922.
  • FENJVES E, YAO S-N, KURACHI K, TAICHMAN LB: Loss ofexpression of a retrovirus-transduced gene in human keratinocytes. J. Invest. Dermatol. (1996) 106:576–578.
  • DENG H, LIN Q, KHAVARI PA: Sustainable cutaneousgene delivery. Nature Biotechnol. (1997) 15:1388–1391.
  • LANGE C, BLANKENSTEIN T: Loss of retroviral geneexpression in bone marrow reconstituted mice correlates with down-regulation of gene expression in long-term culture initiating cells. Gene Ther. (1997) 4:303–308.
  • STRAUSS M: liver-directed gene therapy: prospects and problems. Gene Ther. (1994) 1:156–164.
  • BESTOR T: Gene silencing as a threat to the success of gene therapy. J. Clin. Invest. (2000) 105:409–411.
  • BUELER H: Adeno-associated viral vectors for gene transfer and gene therapy. Biol. Chem. (1999) 380:613–622.
  • HERZOG R, HAGSTROM J, KUNG S et al.: Stable gene transfer and expression of human blood coagulation Factor IX after intramuscular injection of recombinant adeno-associated virus. Proc. Natl. Acad. Sci. USA (1997) 94:5804–5809.
  • CLARK K, SFERRA T, JOHNSON P: Recombinant adeno-associated viral vectors mediate long-term transgene expression in muscle. Hum. Gene Ther. (1997) 8:659–669.
  • BRAUN-FALCO M, DOENECKE A, SMOLA H, HALLEK M: Efficient gene transfer into human keratinocytes with recombinant adeno-associated virus vectors. Gene Ther. (1999) 6:432–441.
  • SALMONS B, GeNZBURG W: Targeting of retroviral vectors for gene therapy. Hum. Gene Ther. (1993) 4:129–141.
  • VILE R, HART I: In vitro and in vivo targeting of geneexpression to melanoma cells. Cancer Res. (1993) 53:962–987.
  • HART I, VILE R: Targeted therapy for malignant melanoma. Curr. Opin. Oncol. (1994) 6:221–225. •
  • CAO G, ZHANG X, HE X, CHEN Q, QI Z: A safe, effective in vivo gene therapy for melanoma using tyrosine promoter-driven cytosine deaminase gene. In vivo (1999) 13:181–187.
  • VILE R, NELSON J, CASTLEDEN S, CHONG H, HART I: Systemic gene therapy of murine melanoma using tissue specific expression of the HSVtk gene involves an immune component. Cancer Res. (1 9 9 4) 54:6228-6234.
  • ALEXANDER M, BIDICHANDANI S, COUSINS F, ROBINSON C, DUFFIE E, AKHURST R: Circulating human Ashley Publications Ltd. All rights reserved.Exp. Opin. Invest. Drugs (2000) 9(9) Factor IX produced in keratin-promoter transgenic mice: a feasibility study for gene therapy of haemophilia B. Hum. Mot. Gen. (1995) 4:993–999.
  • BAILLEUL B, SURANI M, WHITE S et al.: Skin hyp erkera-tosis and papilloma formation in transgenic mice expressing a ras oncogene from a suprabasal keratin promoter. Cell (1990) 62:697–708.
  • PARK B, BROWN C, HU Y et al.: Augmentation of melanoma-specific gene expression using a tandem melanocyte-specific enhancer results in increased cytotoxicity of the purine nucleoside phosporylase gene in melanoma. Hum. Gene Ther. (1999) 10:889–898.
  • PFÜTZNER W, HENGGE UR, JOARI MR, FOSTER RA, VOGEL JC: Selection of keratinocytes transduced with the multidrug resistance gene in an in vitro skin model presents a strategy for enhancing gene expression in vivo. Hum. Gene Ther. (1999) 10:2811–2821.
  • WOLFFE A, MATZKE M: Epigenetics: regulation through repression. Science (1999) 286:481–486.
  • FAUSTINELLA F, KWON H, SERRANO F, BELMONT J, CASKEY C, AGUILAR-CORDOVA E: A new family of murine retroviral vectors with extended multiple cloning sites for gene insertion. Hum. Gene Ther. (1994) 5:307–312.
  • HANTZOPOULOS P, SULLENGER B, UNGERS G, GILBOA E: Improved gene expression upon transfer of the adenosine deaminase minigene outside the transcrip-tional unit of a retroviral vector. Proc. Natl. Acad. Sci. USA (1989) 86:3519–3523.
  • COTSARELIS G, CHENG S, GANG D, SUN T, LAVKER R: Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells. Cell (1989) 57:201–209.
  • JONES P, HARPER S, WATT F: Stem cell patterning and fate in human epidermis. Cell (1995) 80:83–93.
  • HALPRIN K: Epidermal turnover time - a re-examination. Br. J. Dermatol. (1972) 86:14–19.
  • BICKENBACH JR, CHISM E: Selection and extended growth of murine epidermal stem cells in culture. Exp. Cell. Res. (1998) 244:184–195.
  • RAMIREZ A, VIDAL M, BRAVO A, LARCHER F, JORCANO J: A 5'-upstream region of abovine keratine 6 gene confers tissue-specific expression and hyperproliferation-related induction in transgenic mice. Proc. Natl. Acad. Sci. USA (1995) 92:4783–4787.
  • STOLER A, KOPAN R, DUVIC M, FUCHS E: Use of monospecific antisera and cRNAprobes to localize the major changes in keratin expression during normal and abnormal epidermal differentiation. J Cell. Biol. (1988) 107:427–446.
  • MANSBRIDGE J, KNAPP A: Changes in keratinocyte maturation during wound healing. J. Invest. Dermatol (1987) 89:253–263.
  • FOWLIS D, CUT W, JOHNSON S, BALMAIN A, AKHURST R: Altered epidermal cell growth control in vivo by inducible expression of transforming growth factor B1 in the skin of transgenic mice. Cell. Growth Dili (1996) 7:679–687.
  • WANG X, LIEFER K, TSAI S, O'MALLEY B, ROOP DR: Development of gene-switch transgenic mice that inducibly express transforming growth factor B1 in the epidermis. Proc. Nati Acad. Sci. USA (1999) 96:8483–8488.
  • ••In this report, localised, inducible gene expression in skin isachieved by using a keratinocyte-specific promoter (loricrin) to drive a transactivator gene which upon binding of topically applied antiprogestin induces transgene expression.
  • BERTON T, WANG X, ZHOU Z et al.: Characterization of an inducible, epidermal-specific knockout-system: differential expression oflacZin different cre reporter mouse strains. Genesis (2000) 26:160–161.
  • RIVERA V, CLACKSON T, NATESAN S et al.: A humanized system for pharmacologic control of gene expression. Nature Med. (1996) 2:1028–1032.
  • LIBERLES S, DIVERS, AUSTIN D, SCHREIBER S: Inducible gene expression and protein translocation using nontoxic ligands identified by a mammalian three-hybrid screen. Proc. Nati Acad. Sci. USA (1997) 94:7825–7830.

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