oprL Gene Sequencing, Resistance Patterns, Virulence Genes, Quorum Sensing and Antibiotic Resistance Genes of XDR Pseudomonas aeruginosa Isolated from Broiler Chickens

References

• Handley JA, Park SH, Kim SA, Ricke SC. Microbiome profiles of commercial broilers through evisceration and immersion chilling during poultry slaughter and the identification of potential indicator microorganisms. Front Microbiol. 2018;9:345. doi:10.3389/fmicb.2018.00345
   PubMed Web of Science ®Google Scholar
• Abdel-Tawab AA, Nasef SA, Ibrahim OA. Bacteriological and molecular studies on bacteria causing omphalitis in chicks with regard to disinfectant resistance. Glob Veterinaria. 2016;17(6):539–545.
   Google Scholar
• Shukla S, Mishra P. Pseudomonas aeruginosa infection in broiler chicks in Jabalpur. Int J Ext Res. 2015;6:37–39.
   Google Scholar
• Shahat HS, Mohamed H, Al-Azeem A, Mohammed W, Nasef SA. Molecular detection of some virulence genes in Pseudomonas aeruginosa isolated from chicken embryos and broilers with regard to disinfectant resistance. Int J Vet Sci. 2019;2(2):52–70. doi:10.21608/svu.2019.12365.1011
   Google Scholar
• Saad ZA, Nasef SA, Elhariri M, Elhelw R, Ezzeldeen N. Resistance patterns associated with bacterial pathogens causing omphalitis in baby chicks. Biosci Res. 2017;14(4):845–851.
   Google Scholar
• Reynolds D, Kollef M. The epidemiology and pathogenesis and treatment of Pseudomonas aeruginosa infections: an update. Drugs. 2021;81(18):2117–2131. doi:10.1007/s40265-021-01635-6
   PubMed Web of Science ®Google Scholar
• Eraky R, Abd El-Ghany W, Soliman K. Studies on Pseudomonas aeruginosa infection in hatcheries and chicken. J Hellenic Vet Med Soc. 2020;71(1):1953–1962. doi:10.12681/jhvms.22937
   Web of Science ®Google Scholar
• Mesquita CS, Soares-Castro P, Santos PM, Mendez-Vilas A. Pseudomonas aeruginosa: phenotypic flexibility and antimicrobial resistance. Sci Technol Educ. 2013;1:650–665.
   Google Scholar
• Fazeli N, Momtaz H. Virulence gene profiles of multidrug-resistant Pseudomonas aeruginosa isolated from Iranian hospital infections. Iran Red Crescent Med J. 2014;16(10). doi:10.5812/ircmj.15722
   PubMed Web of Science ®Google Scholar
• Jurado-Martín I, Sainz-Mejías M, McClean S. Pseudomonas aeruginosa: an audacious pathogen with an adaptable arsenal of virulence factors. Int J Mol Sci. 2021;22(6):3128. doi:10.3390/ijms22063128
   PubMed Web of Science ®Google Scholar
• Nikbin V, Aslani MM, Sharafi Z, Hashemipour M, Shahcheraghi F, Ebrahimipour G. Molecular identification and detection of virulence genes among Pseudomonas aeruginosa isolated from different infectious origins. Iran J Microbiol. 2012;4(3):118.
   PubMedGoogle Scholar
• Gong Q, Ruan M, Niu M, Qin C, Hou Y, Guo J. Immune efficacy of DNA vaccines based on oprL and oprF genes of Pseudomonas aeruginosa in chickens. Poult Sci. 2018;97(12):4219–4227. doi:10.3382/ps/pey307
   PubMed Web of Science ®Google Scholar
• Rocha AJ, Barsottini M, Rocha RR, Laurindo MV, Moraes FL, Rocha SL. Pseudomonas aeruginosa: virulence factors and antibiotic resistance genes. Braz Arch Biol Technol. 2019;62. doi:10.1590/1678-4324-2019180503
   Web of Science ®Google Scholar
• Algammal AM, Hashem HR, Al-Otaibi AS, et al. Emerging MDR-Mycobacterium avium subsp. avium in house-reared domestic birds as the first report in Egypt. BMC Microbiol. 2021;21(1):1–11. doi:10.1186/s12866-021-02287-y
   PubMed Web of Science ®Google Scholar
• Elbehiry A, Marzouk E, Aldubaib M, et al. Pseudomonas species prevalence, protein analysis, and antibiotic resistance: an evolving public health challenge. AMB Express. 2022;12(1):1–14. doi:10.1186/s13568-022-01390-1
   PubMed Web of Science ®Google Scholar
• Algammal AM, Abo Hashem ME, Alfifi KJ, et al. Sequence analysis, antibiogram profile, virulence and antibiotic resistance genes of XDR and MDR Gallibacterium anatis isolated from layer chickens in Egypt. Infect Drug Resist. 2022:4321–4334. doi:10.2147/IDR.S377797
   PubMedGoogle Scholar
• Algammal AM, Ibrahim RA, Alfifi KJ, et al. A first report of molecular typing, virulence traits, and phenotypic and genotypic resistance patterns of newly emerging XDR and MDR Aeromonas veronii in Mugil seheli. Pathogens. 2022;11(11):1262. doi:10.3390/pathogens11111262
   PubMed Web of Science ®Google Scholar
• Langendonk RF, Neill DR, Fothergill JL. The building blocks of antimicrobial resistance in Pseudomonas aeruginosa: implications for current resistance-breaking therapies. Front Cell Infect Microbiol. 2021;11:665759. doi:10.3389/fcimb.2021.665759
   PubMed Web of Science ®Google Scholar
• Peymani A, Naserpour-Farivar T, Zare E, Azarhoosh K. Distribution of blaTEM, blaSHV, and blaCTX-M genes among ESBL-producing P. aeruginosa isolated from Qazvin and Tehran hospitals, Iran. J Prev Med Hyg. 2017;58(2):E155.
   PubMedGoogle Scholar
• Heir E, Moen B, Åsli AW, Sunde M, Langsrud S. Antibiotic resistance and phylogeny of Pseudomonas spp. isolated over three decades from chicken meat in the Norwegian food chain. Microorganisms. 2021;9(2):207. doi:10.3390/microorganisms9020207
   PubMed Web of Science ®Google Scholar
• Meng L, Liu H, Lan T, et al. Antibiotic resistance patterns of Pseudomonas spp. isolated from raw milk revealed by whole genome sequencing. Front Microbiol. 2020;11:1005. doi:10.3389/fmicb.2020.01005
   PubMedGoogle Scholar
• Mac Faddin JF. Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Williams & Wilkins; 1985.
   Google Scholar
• Xu J, Moore JE, Murphy PG, Millar BC, Elborn JS. Early detection of Pseudomonas aeruginosa–comparison of conventional versus molecular (PCR) detection directly from adult patients with cystic fibrosis (CF). Ann Clin Microbiol Antimicrob. 2004;3(1):1–5. doi:10.1186/1476-0711-3-21
   PubMedGoogle Scholar
• Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci. 2004;2004:11030–11035.
   Google Scholar
• Clinical Laboratory Standards Institute. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. 4th ed. In: CLSI Supplement VET08. Wayne: Clinical Laboratory Standards Institute; 2018.
   Google Scholar
• Magiorakos A-P, Srinivasan A, Carey RB, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012;18(3):268–281. doi:10.1111/j.1469-0691.2011.03570.x
   PubMed Web of Science ®Google Scholar
• Krumperman PH. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Appl Environ Microbiol. 1983;46(1):165–170. doi:10.1128/aem.46.1.165-170.1983
   PubMed Web of Science ®Google Scholar
• Sabharwal N, Dhall S, Chhibber S, Harjai K. Molecular detection of virulence genes as markers in Pseudomonas aeruginosa isolated from urinary tract infections. Int J Mol Epidemiol Genet. 2014;5(3):125.
   PubMedGoogle Scholar
• Winstanley C, Kaye SB, Neal TJ, Chilton HJ, Miksch S, Hart CA. Genotypic and phenotypic characteristics of Pseudomonas aeruginosa isolates associated with ulcerative keratitis. J Med Microbiol. 2005;54(6):519–526. doi:10.1099/jmm.0.46005-0
   PubMed Web of Science ®Google Scholar
• Finnan S, Morrissey JP, O’Gara F, Boyd EF. Genome diversity of Pseudomonas aeruginosa isolates from cystic fibrosis patients and the hospital environment. J Clin Microbiol. 2004;42(12):5783–5792. doi:10.1128/JCM.42.12.5783-5792.2004
   PubMed Web of Science ®Google Scholar
• Colom K, Pérez J, Alonso R, Fernández-Aranguiz A, Lariño E, Cisterna R. Simple and reliable multiplex PCR assay for detection of blaTEM, blaSHV and blaOXA–1 genes in Enterobacteriaceae. FEMS Microbiol Lett. 2003;223(2):147–151. doi:10.1016/S0378-1097(03)00306-9
   PubMed Web of Science ®Google Scholar
• Archambault M, Petrov P, Hendriksen RS, et al. Molecular characterization and occurrence of extended-spectrum β-lactamase resistance genes among Salmonella enterica serovar Corvallis from Thailand, Bulgaria, and Denmark. Microb Drug Resist. 2006;12(3):192–198. doi:10.1089/mdr.2006.12.192
   PubMed Web of Science ®Google Scholar
• Randall L, Cooles S, Osborn M, Piddock L, Woodward MJ. Antibiotic resistance genes, integrons and multiple antibiotic resistance in thirty-five serotypes of Salmonella enterica isolated from humans and animals in the UK. J Antimicrob Chemother. 2004;53(2):208–216. doi:10.1093/jac/dkh070
   PubMed Web of Science ®Google Scholar
• Ibekwe AM, Murinda SE, Graves AK. Genetic diversity and antimicrobial resistance of Escherichia coli from human and animal sources uncovers multiple resistances from human sources. PLoS One. 2011;6(6):e20819. doi:10.1371/journal.pone.0020819
   PubMed Web of Science ®Google Scholar
• Abdelmoez N, Shawky M, Abdelhady H, Lebdah M, Salama S. Isolation and identification of some possible causative agents of swollen head syndrome (SHS) in broiler chickens in Egypt. Slov Vet Res. 2019;56:542.
   Web of Science ®Google Scholar
• Abd El-Tawab A, El-Hofy F, Khater D, Al-Adl M. Virulence, resistance genes detection and sequencing of gyrA gene of Pseudomonas aeruginosa isolated from chickens and human in Egypt. Nat Sci. 2018;16(2):32–39.
   Google Scholar
• Abd El-Ghany WA. Pseudomonas aeruginosa infection of avian origin: zoonosis and one health implications. Vet World. 2021;14(8):2155. doi:10.14202/vetworld.2021.2155-2159
   PubMed Web of Science ®Google Scholar
• Algammal AM, Mabrok M, Sivaramasamy E, et al. Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and blaTEM, blaCTX-M, and tetA antibiotic-resistance genes. Sci Rep. 2020;10(1):1–12. doi:10.1038/s41598-020-72264-4
   PubMedGoogle Scholar
• Kumar A, Chua K-L, Schweizer HP. Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b. Antimicrob Agents Chemother. 2006;50(10):3460–3463. doi:10.1128/AAC.00440-06
   PubMed Web of Science ®Google Scholar
• Kousar S, Rehman N, Javed A, et al. Intensive poultry farming practices influence antibiotic resistance profiles in Pseudomonas aeruginosa inhabiting nearby soils. Infect Drug Resist. 2021;14:4511. doi:10.2147/IDR.S324055
   PubMed Web of Science ®Google Scholar
• Mohamed HM, Alnasser SM, Abd-Elhafeez HH, Alotaibi M, Batiha GE-S, Younis W. Detection of β-lactamase resistance and biofilm genes in pseudomonas species isolated from chickens. Microorganisms. 2022;10(10):1975. doi:10.3390/microorganisms10101975
   PubMed Web of Science ®Google Scholar
• Sans-Serramitjana E, Fusté E, Martínez-Garriga B, et al. Killing effect of nanoencapsulated colistin sulfate on Pseudomonas aeruginosa from cystic fibrosis patients. J Cystic Fibrosis. 2016;15(5):611–618. doi:10.1016/j.jcf.2015.12.005
   PubMed Web of Science ®Google Scholar
• Rafique A, Andleeb S, Ghous T, Shahzad N, Shafique I. Antibacterial activity of traditional herbs and standard antibiotics against poultry associated Pseudomonas aeruginosa. J Pharm Sci Innov. 2012;1(5):12–16.
   Google Scholar
• Makharita RR, El-Kholy I, Hetta HF, et al. Antibiogram and genetic characterization of carbapenem-resistant gram-negative pathogens incriminated in healthcare-associated infections. Infect Drug Resist. 2020;13:3991. doi:10.2147/IDR.S276975
   PubMed Web of Science ®Google Scholar
• Langaee TY, Gagnon L, Huletsky A. Inactivation of the ampD gene in Pseudomonas aeruginosa leads to moderate-basal-level and hyperinducible AmpC β-lactamase expression. Antimicrob Agents Chemother. 2000;44(3):583–589. doi:10.1128/AAC.44.3.583-589.2000
   PubMed Web of Science ®Google Scholar
• Al-Dahmoshi HO, Al-Khafaji NS, Jeyad AA, Shareef HK, Al-Jebori RF. Molecular detection of some virulence traits among Pseudomonas aeruginosa isolates, Hilla-Iraq. Biomed Pharmacol J. 2018;11(2):835–842. doi:10.13005/bpj/1439
   Google Scholar
• Bakheet AA, Torra DE. Detection of Pseudomonas aeruginosa in dead chicken embryo with reference to pathological changes and virulence genes. Alexandria J Vet Sci. 2020;65(1):81. doi:10.5455/ajvs.101343
   Google Scholar
• Qian Z, Hui P, Han L, et al. Serotypes and virulence genes of Pseudomonas aeruginosa isolated from mink and its pathogenicity in mink. Microb Pathog. 2020;139:103904. doi:10.1016/j.micpath.2019.103904
   PubMed Web of Science ®Google Scholar
• Douraghi M, Ghasemi F, Dallal MS, Rahbar M, Rahimiforoushani A. Molecular identification of Pseudomonas aeruginosa recovered from cystic fibrosis patients. J Prev Med Hyg. 2014;55(2):50.
   PubMedGoogle Scholar
• Vanderwoude J, Fleming D, Azimi S, Trivedi U, Rumbaugh KP, Diggle SP. The evolution of virulence in Pseudomonas aeruginosa during chronic wound infection. Proc Royal Soc B. 2020;287(1937):20202272. doi:10.1098/rspb.2020.2272
   Google Scholar
• Iiyama K, Takahashi E, Lee JM, et al. Alkaline protease contributes to pyocyanin production in Pseudomonas aeruginosa. FEMS Microbiol Lett. 2017;364(7). doi:10.1093/femsle/fnx051
   PubMed Web of Science ®Google Scholar
• Butterworth MB, Zhang L, Heidrich EM, Myerburg MM, Thibodeau PH. Activation of the epithelial sodium channel (ENaC) by the alkaline protease from Pseudomonas aeruginosa. J Biol Chem. 2012;287:32556–32565. doi:10.1074/jbc.M112.369520
   PubMed Web of Science ®Google Scholar
• Hartmann A, Schikora A. Quorum sensing of bacteria and trans-kingdom interactions of N-acyl homoserine lactones with eukaryotes. J Chem Ecol. 2012;38(6):704–713. doi:10.1007/s10886-012-0141-7
   PubMed Web of Science ®Google Scholar
• O’Loughlin CT, Miller LC, Siryaporn A, Drescher K, Semmelhack MF, Bassler BL. A quorum-sensing inhibitor blocks Pseudomonas aeruginosa virulence and biofilm formation. Proc Natl Acad Sci. 2013;110(44):17981–17986. doi:10.1073/pnas.1316981110
   PubMed Web of Science ®Google Scholar
• Khorvash F, Yazdani M, Shabani S, Soudi A. Pseudomonas aeruginosa-producing metallo-β-lactamases (VIM, IMP, SME, and AIM) in the clinical isolates of intensive care units, a university hospital in Isfahan, Iran. Adv Biomed Res. 2017;6:25.
   PubMedGoogle Scholar
• Sanz-García F, Hernando-Amado S, Martínez JL. Mutation-driven evolution of Pseudomonas aeruginosa in the presence of either ceftazidime or ceftazidime-avibactam. Antimicrob Agents Chemother. 2018;62(10):e01379–01318. doi:10.1128/AAC.01379-18
   PubMed Web of Science ®Google Scholar
• Krishnan G, Sethumadhavan A, Muthusamy S, Mani M. Antibiotic resistant clinical isolates of Pseudomonas aeruginosa harbor LasA gene. Internet J Microbiol. 2019;16:125.
   Google Scholar
• Cho J-K, Kim J-H, Kim J-M, Park C-K, Kim K-S. Antimicrobial resistance and distribution of resistance gene in Enterobacteriaceae and Pseudomonas aeruginosa isolated from dogs and cats. Korean J Vet Service. 2013;36(3):171–180. doi:10.7853/kjvs.2013.36.3.171
   Google Scholar
• Botelho J, Grosso F, Peixe L. Antibiotic resistance in Pseudomonas aeruginosa–mechanisms, epidemiology and evolution. Drug Resist Updates. 2019;44:100640. doi:10.1016/j.drup.2019.07.002
   PubMed Web of Science ®Google Scholar
• Pang Z, Raudonis R, Glick BR, Lin T-J, Cheng Z. Antibiotic resistance in Pseudomonas aeruginosa: mechanisms and alternative therapeutic strategies. Biotechnol Adv. 2019;37(1):177–192. doi:10.1016/j.biotechadv.2018.11.013
   PubMed Web of Science ®Google Scholar
• Gajdács M, Baráth Z, Kárpáti K, et al. No correlation between biofilm formation, virulence factors, and antibiotic resistance in Pseudomonas aeruginosa: results from a laboratory-based in vitro study. Antibiotics. 2021;10:1134. doi:10.3390/antibiotics10091134
   Google Scholar