Improving Blood Culture Quality with a Medical Staff Educational Program: A Prospective Cohort Study

References

• Rudd KE, Johnson SC, Agesa KM, et al. Global, regional, and national sepsis incidence and mortality, 1990–2017: analysis for the global burden of disease study. Lancet. 2020;395(10219):200–211. doi:10.1016/S0140-6736(19)32989-7
   PubMed Web of Science ®Google Scholar
• Timsit JF, Ruppé E, Barbier F, Tabah A, Bassetti M. Bloodstream infections in critically ill patients: an expert statement. Intensive Care Med. 2020;46(2):266–284. doi:10.1007/s00134-020-05950-6
   PubMed Web of Science ®Google Scholar
• Kumar A, Roberts D, Wood KE, et al. Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med. 2006;34(6):1589–1596. doi:10.1097/01.CCM.0000217961.75225.E9
   PubMed Web of Science ®Google Scholar
• Pliakos EE, Andreatos N, Shehadeh F, Ziakas PD, Mylonakis E. The cost-effectiveness of rapid diagnostic testing for the diagnosis of bloodstream infections with or without antimicrobial stewardship. Clin Microbiol Rev. 2018;31(3). doi:10.1128/CMR.00095-17
   PubMed Web of Science ®Google Scholar
• Evans L, Rhodes A, Alhazzani W, et al. Surviving sepsis campaign: international guidelines for management of sepsis and septic shock 2021. Crit Care Med. 2021;49(11):e1063–e1143. doi:10.1097/CCM.0000000000005337
   PubMed Web of Science ®Google Scholar
• Dubourg G, Lamy B, Ruimy R. Rapid phenotypic methods to improve the diagnosis of bacterial bloodstream infections: meeting the challenge to reduce the time to result. Clin Microbiol Infect. 2018;24(9):935–943. doi:10.1016/j.cmi.2018.03.031
   PubMed Web of Science ®Google Scholar
• Lamy B, Ferroni A, Henning C, Cattoen C, Laudat P. How to: accreditation of blood cultures’ proceedings. A clinical microbiology approach for adding value to patient care. Clin Microbiol Infect. 2018;24(9):956–963. doi:10.1016/j.cmi.2018.01.011
   PubMed Web of Science ®Google Scholar
• Cockerill FR 3rd, Wilson JW, Vetter EA, et al. Optimal testing parameters for blood cultures. Clin Infect Dis. 2004;38(12):1724–1730. doi:10.1086/421087
   PubMed Web of Science ®Google Scholar
• McElvania SK. Manual of Clinical Microbiology. 12th ed. Washington, DC: ASM Press; 2019.
   Google Scholar
• Lamy B, Dargère S, Arendrup MC, Parienti JJ, Tattevin P. How to optimize the use of blood cultures for the diagnosis of bloodstream infections? A state-of-the art. Front Microbiol. 2016;7:697. doi:10.3389/fmicb.2016.00697
   PubMed Web of Science ®Google Scholar
• Dargère S, Cormier H, Verdon R. Contaminants in blood cultures: importance, implications, interpretation and prevention. Clin Microbiol Infect. 2018;24(9):964–969. doi:10.1016/j.cmi.2018.03.030
   PubMed Web of Science ®Google Scholar
• Institute CaLS. Principles and procedures for blood cultures: approved guidelines. In: CLSI Document M47-A. Wayne, PA: Clinical and Laboratory Standards Institute; 2007.
   Google Scholar
• Tajima T, Asai Y, Endo M, et al. Rate of blood culture submissions in Japan as an indicator of bloodstream infections. J Infect Chemother. 2021;27(8):1270–1272. doi:10.1016/j.jiac.2021.04.019
   PubMed Web of Science ®Google Scholar
• Adamik M, Hutchins A, Mangilit J, Katzin B, Totty H, Deol P. Effect of delayed entry on performance of the BACT/ALERT FAN PLUS bottles in the BACT/ALERT VIRTUO blood culture system. Eur J Clin Microbiol Infect Dis. 2021;40(4):699–705. doi:10.1007/s10096-020-04042-z
   PubMed Web of Science ®Google Scholar
• Dien Bard J, Chang TP, Yee R, et al. The addition of anaerobic blood cultures for pediatric patients with concerns for bloodstream infections: prevalence and time to positive cultures. J Clin Microbiol. 2020;58(9). doi:10.1128/JCM.01844-19
   Web of Science ®Google Scholar
• Willems E, Smismans A, Cartuyvels R, et al. The preanalytical optimization of blood cultures: a review and the clinical importance of benchmarking in 5 Belgian hospitals. Diagn Microbiol Infect Dis. 2012;73(1):1–8. doi:10.1016/j.diagmicrobio.2012.01.009
   PubMed Web of Science ®Google Scholar
• McLeod CG. Reducing blood culture contamination in the emergency department. J Nurs Care Qual. 2020;35(3):245–251. doi:10.1097/NCQ.0000000000000441
   PubMed Web of Science ®Google Scholar
• Steiner K, Baron-Stefaniak J, Hirschl AM, Barousch W, Willinger B, Baron DM. Education of medical personnel optimizes filling volume of blood culture bottles without negatively affecting microbiology testing. BMC Health Serv Res. 2020;20(1):1105. doi:10.1186/s12913-020-05959-z
   PubMed Web of Science ®Google Scholar
• Haiyan Cao YL. Investigation of blood culture specimen inspection situation in a general hospital. Int J Lab Med. 2012;33(17):2.
   Google Scholar
• Wang HE, Jones AR, Donnelly JP. Revised national estimates of emergency department visits for sepsis in the United States. Crit Care Med. 2017;45(9):1443–1449. doi:10.1097/CCM.0000000000002538
   PubMed Web of Science ®Google Scholar
• Miller JM, Binnicker MJ, Campbell S, et al. A guide to utilization of the microbiology laboratory for diagnosis of infectious diseases: 2018 update by the Infectious Diseases Society of America and the American Society for microbiology. Clin Infect Dis. 2018;67(6):e1–e94. doi:10.1093/cid/ciy381
   PubMed Web of Science ®Google Scholar
• Lee A, Mirrett S, Reller LB, Weinstein MP. Detection of bloodstream infections in adults: how many blood cultures are needed? J Clin Microbiol. 2007;45(11):3546–3548. doi:10.1128/JCM.01555-07
   PubMed Web of Science ®Google Scholar
• Ehrenstein BP, Ehrenstein V, Henke C, et al. Risk factors for negative blood cultures in adult medical inpatients--a retrospective analysis. BMC Infect Dis. 2008;8:148. doi:10.1186/1471-2334-8-148
   PubMed Web of Science ®Google Scholar
• Alahmadi YM, McElnay JC, Kearney MP, et al. Tackling the problem of blood culture contamination in the intensive care unit using an educational intervention. Epidemiol Infect. 2015;143(9):1964–1971. doi:10.1017/S0950268814003008
   PubMed Web of Science ®Google Scholar
• Ekwall-Larson A, Yu D, Dinnétz P, Nordqvist H, Özenci V. Single-site sampling versus multisite sampling for blood cultures: a retrospective clinical study. J Clin Microbiol. 2022;60(2):e0193521. doi:10.1128/JCM.01935-21
   PubMed Web of Science ®Google Scholar
• Mermel LA, Maki DG. Detection of bacteremia in adults: consequences of culturing an inadequate volume of blood. Ann Intern Med. 1993;119(4):270–272. doi:10.7326/0003-4819-119-4-199308150-00003
   PubMed Web of Science ®Google Scholar
• van Ingen J, Hilt N, Bosboom R. Education of phlebotomy teams improves blood volume in blood culture bottles. J Clin Microbiol. 2013;51(3):1020–1021.
   PubMed Web of Science ®Google Scholar
• Saito T, Iinuma Y, Takakura S, et al. Delayed insertion of blood culture bottles into automated continuously monitoring blood culture systems increases the time from blood sample collection to the detection of microorganisms in bacteremic patients. J Infect Chemother. 2009;15(1):49–53. doi:10.1007/s10156-008-0664-6
   PubMed Web of Science ®Google Scholar
• Venturelli C, Righi E, Borsari L, et al. Impact of pre-analytical time on the recovery of pathogens from blood cultures: results from a large retrospective survey. PLoS One. 2017;12(1):e0169466. doi:10.1371/journal.pone.0169466
   PubMed Web of Science ®Google Scholar
• Elvy J, Walker D, Haremza E, Ryan K, Morris AJ. Blood culture quality assurance: what Australasian laboratories are measuring and opportunities for improvement. Pathology. 2021;53(4):520–529. doi:10.1016/j.pathol.2020.09.020
   PubMed Web of Science ®Google Scholar
• Rivers E, Nguyen B, Havstad S, et al. Early goal-directed therapy in the treatment of severe sepsis and septic shock. N Engl J Med. 2001;345(19):1368–1377. doi:10.1056/NEJMoa010307
   PubMed Web of Science ®Google Scholar
• Tabak YP, Vankeepuram L, Ye G, Jeffers K, Gupta V, Murray PR. Blood culture turnaround time in U.S. acute care hospitals and implications for laboratory process optimization. J Clin Microbiol. 2018;56(12). doi:10.1128/JCM.00500-18
   PubMed Web of Science ®Google Scholar
• Chen Y, Ji J, Ying C, et al. Blood bacterial resistant investigation collaborative system (BRICS) report: a national surveillance in China from 2014 to 2019. Antimicrob Resist Infect Control. 2022;11(1):17. doi:10.1186/s13756-022-01055-5
   PubMed Web of Science ®Google Scholar