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ORIGINAL RESEARCH

Antimicrobial Resistance and Virulence Gene Profile of Clinical Staphylococcus aureus: A Multi-Center Study from Ethiopia

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Pages 4835-4844 | Received 02 May 2023, Accepted 12 Jul 2023, Published online: 25 Jul 2023

References

  • Tong SYC, Davis JS, Eichenberger E, Holland TL, Fowler VG. Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev. 2015;28(3):603–661. doi:10.1128/CMR.00134-14
  • Nimmo GR. USA300 abroad: global spread of a virulent strain of community-associated methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect. 2012;18(8):725–734. doi:10.1111/j.1469-0691.2012.03822.x
  • Kobayashi SD, Malachowa N, Deleo FR. Pathogenesis of Staphylococcus aureus abscesses. Am J Pathol. 2015;185(6):1518–1527. doi:10.1016/j.ajpath.2014.11.030
  • Darboe S, Dobreniecki S, Jarju S, et al. Prevalence of Panton-Valentine Leukocidin (PVL) and antimicrobial resistance in community-acquired clinical staphylococcus aureus in an urban Gambian hospital: a 11-year period retrospective pilot study. Front Cell Infect Microbiol. 2019;9:1–7. doi:10.3389/fcimb.2019.00170
  • Li Z, Stevens DL, Hamilton SM, et al. Fatal S. aureus hemorrhagic pneumonia: genetic analysis of a unique clinical isolate producing both PVL and TSST-1. PLoS One. 2011;6(11):154.
  • Chuang-Smith ON, Schlievert PM. Staphylococcal enterotoxin C subtypes are differentially associated with human infections and immunobiological activities. mSphere. 2021;6(1). doi:10.1128/mSphere.01153-20
  • Ortega E, Abriouel H, Lucas R, Gálvez A. Multiple roles of staphylococcus aureus enterotoxins: pathogenicity, superantigenic activity, and correlation to antibiotic resistance. Toxins. 2010;2:2117–2131. doi:10.3390/toxins2082117
  • Chambers HF, DeLeo FR. Waves of resistance: Staphylococcus aureus in the antibiotic era. Nat Rev Microbiol. 2009;7:629–641. doi:10.1038/nrmicro2200
  • Lowy FD. Antimicrobial resistance: the example of Staphylococcus aureus. J Clin Invest. 2003;111(9):1265–1273. doi:10.1172/JCI18535
  • Deyno S, Fekadu S, Astatkie A. Resistance of Staphylococcus aureus to antimicrobial agents in Ethiopia: a meta-analysis. Antimicrob Resist Infect Control. 2017;6(1). doi:10.1186/s13756-017-0243-7
  • Juayang AC, De Los Reyes GB, De La Rama AJG, Gallega CT. Antibiotic resistance profiling of Staphylococcus aureus isolated from clinical specimens in a tertiary hospital from 2010 to 2012. Interdiscip Perspect Infect Dis. 2014;2014:1–4. doi:10.1155/2014/898457
  • Pantosti A, Sanchini A, Monaco M. Mechanisms of antibiotic resistance in Staphylococcus aureus. Future Microbiol. 2007;2(3):323–334. doi:10.2217/17460913.2.3.323
  • Guo Y, Song G, Sun M, Wang J, Wang Y. Prevalence and therapies of antibiotic-resistance in Staphylococcus aureus. Front Cell Infect Microbiol. 2020;10:1–11. doi:10.3389/fcimb.2020.00107
  • Mama M, Abdissa A, Sewunet T. Antimicrobial susceptibility pattern of bacterial isolates from wound infection and their sensitivity to alternative topical agents at Jimma University Specialized Hospital, South-West Ethiopia. Ann Clin Microbiol Antimicrob. 2014;13(1). doi:10.1186/1476-0711-13-14
  • Dilnessa T, Bitew A. Prevalence and antimicrobial susceptibility pattern of methicillin resistant Staphylococcus aureus isolated from clinical samples at Yekatit 12 Hospital Medical College, Addis Ababa, Ethiopia. BMC Infect Dis. 2016;16(1). doi:10.1186/s12879-016-1742-5
  • Desta K, Aklillu E, Gebrehiwot Y, et al. High levels of methicillin-resistant Staphylococcus aureus carriage among healthcare workers at a teaching hospital in Addis Ababa Ethiopia: first evidence using mecA detection. Infect Drug Resist. 2022;15:3135–3147. doi:10.2147/IDR.S360123
  • Verdú-Expósito C, Romanyk J, Cuadros-González J, et al. Study of susceptibility to antibiotics and molecular characterization of high virulence Staphylococcus aureus strains isolated from a rural hospital in Ethiopia. PLoS One. 2020;15(3):1–17. doi:10.1371/journal.pone.0230031
  • Ethiopia AMR surveillance plan_final. Available from: https://www.ephi.gov.et/images/pictures/download2010/Ethiopia-AMR-Surveillance-Plan_Final.pdf. Accessed July 13, 2023.
  • Weinstein MP. M100-Performance Standards for Antimicrobial Susceptibility Testing. 28th ed. Clinical and Laboratory; 2018.
  • Stegger M, Andersen PS, Kearns A, et al. Rapid detection, differentiation and typing of methicillin-resistant Staphylococcus aureus harbouring either mecA or the new mecA homologue mecALGA251. Clin Microbiol Infect. 2012;18(4):395–400. doi:10.1111/j.1469-0691.2011.03715.x
  • Løvseth A, Loncarevic S, Berdal KG. Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates. J Clin Microbiol. 2004;42(8):3869–3872. doi:10.1128/JCM.42.8.3869-3872.2004
  • Risch M, Radjenovic D, Han JN, Wydler M, Nydegger U, Risch L. Comparison of MALDI TOF with conventional identification of clinically relevant bacteria. Swiss Med Wkly. 2010;140. doi:10.4414/smw.2010.13095
  • El-Bouri K, Johnston S, Rees E, et al. Comparison of bacterial identification by MALDI-TOF mass spectrometry and conventional diagnostic microbiology methods: agreement, speed and cost implications. Br J Biomed Sci. 2012;69(2):47–55. doi:10.1080/09674845.2012.12002436
  • Ayeni FA, Odumosu BT. False identification of other microorganisms as staphylococcus aureus in Southern Nigeria. Trop J Pharma Res. 2016;15(9):1941–1945. doi:10.4314/tjpr.v15i9.19
  • Eyasu T, Tesfu K, Daniel A, et al. Phenotypic and genotypic characterization of Staphylococcus aureus isolates recovered from bovine milk in central highlands of Ethiopia. Afr J Microbiol Res. 2015;9(44):2209–2217. doi:10.5897/AJMR2015.7562
  • Nyasinga J, Kyany’a C, Okoth R, et al. A six-member SNP assay on the iPlex MassARRAY platform provides a rapid and affordable alternative for typing major African Staphylococcus aureus types. Access Microbiol. 2019;1(3). doi:10.1099/acmi.0.000018
  • Mama M, Aklilu A, Misgna K, Tadesse M, Alemayehu E. Methicillin- and inducible clindamycin-resistant Staphylococcus aureus among patients with wound infection attending Arba Minch Hospital, South Ethiopia. Int J Microbiol. 2019;2019:1–9. doi:10.1155/2019/2965490
  • Mišić M, Čukić J, Vidanović D, et al. Prevalence of genotypes that determine resistance of staphylococci to macrolides and lincosamides in Serbia. Front Public Health. 2017;5:1–8. doi:10.3389/fpubh.2017.00200
  • Baguma A, Musinguzi B, Mpeirwe M, Bazira J. Clindamycin resistance among methicillin resistant Staphylococcus aureus isolated from human and respective household swine in Greater Kabale Region—South Western Uganda. Adv Infect Dis. 2019;09(04):285–294.
  • Kishk RM, Anani MM, Nemr NA, Soliman NM, Fouad MM. Inducible clindamycin resistance in clinical isolates of staphylococcus aureus in Suez Canal University Hospital, Ismailia. Egypt J Infect Dev Ctries. 2020;14(11):1281–1287. doi:10.3855/jidc.12250
  • Shah DA, Wasim S, Abdullah FE. Comparison of Oxacillin and Cefoxitin for the detection of mecA gene to determine Methicillin resistance in coagulase negative staphylococci (CoNs). J Coll Physicians Surg Pak. 2017;27(8):520–522.
  • Gitau W, Masika M, Musyoki M, Museve B, Mutwiri T. Antimicrobial susceptibility pattern of Staphylococcus aureus isolates from clinical specimens at Kenyatta National Hospital. BMC Res Notes. 2018;11(1):1–5. doi:10.1186/s13104-018-3337-2
  • Dhungel S, Rijal KR, Yadav B, et al. Methicillin-resistant Staphylococcus aureus (MRSA): prevalence, antimicrobial susceptibility pattern, and detection of mec A gene among cardiac patients from a Tertiary Care Heart Center in Kathmandu, Nepal Infect Dis. 2021;14:117863372110373.
  • Watkins RR, Holubar M, David MZ. Antimicrobial resistance in methicillin-resistant Staphylococcus aureus to newer antimicrobial agents. Antimicrob Agents Chemother. 2019;63(12). doi:10.1128/AAC.01216-19
  • Haggag MG, Aboelnour AE, Al-Kaffas M. MRSA screening and spa gene detection in isolates from healthcare workers at ophthalmology hospital in Egypt. Bull Natl Res Cent. 2019;43(1). doi:10.1186/s42269-019-0253-0
  • Votintseva AA, Fung R, Miller RR, et al. Prevalence of Staphylococcus aureus protein A (spa) mutants in the community and hospitals in Oxfordshire. BMC Microbiol. 2014;14(1):63. doi:10.1186/1471-2180-14-63
  • Iliya S, Mwangi J, Maathai R, Muriuki M, Wainaina C. Molecular detection of panton valentine leukocidin toxin in clinical isolates of Staphylococcus aureus from Kiambu County, Kenya. Int J Microbiol. 2020;2020:1–8. doi:10.1155/2020/3106747
  • Hardy C, Osei L, Basset T, Elenga N. Bone and joint infections with Staphylococcus aureus strains producing Panton-Valentine Leukocidin in French Guiana. Medicine. 2019;98(27):e16015. doi:10.1097/MD.0000000000016015
  • Sadat A, Shata RR, Farag AMM, et al. Prevalence and characterization of PVL-positive staphylococcus aureus isolated from raw cow’s milk. Toxins. 2022;14(2):1–17. doi:10.3390/toxins14020097
  • Seyoum ET, Mekonene TK, Woldetsadik DA, Zewudie BM, Gebreyes WA. Enterotoxin gene profile of staphylococcus aureus isolates recovered from bovine milk produced in central Ethiopia. J Infect Dev Ctries. 2016;10(2):138–142. doi:10.3855/jidc.6797
  • Yahya Ahmed M, Abdalbagi Ali H, Mohammed Taher Gorish B, et al. Molecular detection of Staphylococcal enterotoxins and mecA genes products in selected food samples collected from different areas in Khartoum State. Int J Microbiol. 2021;2021:1–9. doi:10.1155/2021/5520573
  • Tegegne DT, Mamo G, Waktole H, Messele YE. Molecular characterization of virulence factors in Staphylococcus aureus isolated from bovine subclinical mastitis in central Ethiopia. Ann Microbiol. 2021;71(1). doi:10.1186/s13213-021-01639-3
  • Molla M, Temesgen K, Seyoum T, Melkamu M. Surgical site infection and associated factors among women underwent cesarean delivery in Debretabor General Hospital, Northwest Ethiopia: hospital based cross sectional study. BMC Pregnancy Childbirth. 2019;19(1):1–10. doi:10.1186/s12884-019-2442-0
  • Demir D, Karabay O, Güven M, Kayabasoğlu G, Yilmaz MS. Do Staphylococcus aureus superantigens play a role in the pathogenesis of otitis media with effusion in children? Int J Pediatr Otorhinolaryngol. 2016;84:71–74. doi:10.1016/j.ijporl.2016.02.028
  • Xu J, Du Q, Shu Y, Ji J, Dai C. Bacteriological profile of chronic suppurative otitis media and antibiotic susceptibility in a tertiary care hospital in Shanghai, China. Ear Nose Throat J. 2021;100(9):NP391–6. doi:10.1177/0145561320923823
  • Deodhar D, Varghese G, Balaji V, et al. Prevalence of toxin genes among the clinical isolates of Staphylococcus aureus and its clinical impact. J Glob Infect Dis. 2015;7(3):97–102. doi:10.4103/0974-777X.162234