Within-Host Resistance and Virulence Evolution of a Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae ST11 Under Antibiotic Pressure

References

• Navon-Venezia S, Kondratyeva K, Carattoli A. Klebsiella pneumoniae: a major worldwide source and shuttle for antibiotic resistance. Fems Microbiol Rev. 2017;41(3):252–275. doi:10.1093/femsre/fux013
   PubMed Web of Science ®Google Scholar
• Gu D, Dong N, Zheng Z, et al. A fatal outbreak of st11 carbapenem-resistant hypervirulent Klebsiella pneumoniae in a Chinese hospital: a molecular epidemiological study. Lancet Infect Dis. 2018;18(1):37–46. doi:10.1016/S1473-3099(17)30489-9
   PubMed Web of Science ®Google Scholar
• Tian D, Wang M, Zhou Y, Hu D, Jiang X. Genetic diversity and evolution of the virulence plasmids encoding aerobactin and salmochelin in Klebsiella pneumoniae. Virulence. 2021;12(1):1323–1333. doi:10.1080/21505594.2021.1924019
   PubMed Web of Science ®Google Scholar
• Lan P, Jiang Y, Zhou J, Yu Y. A global perspective on the convergence of hypervirulence and carbapenem resistance in Klebsiella pneumoniae. J Glob Antimicrob Resist. 2021;25:26–34. doi:10.1016/j.jgar.2021.02.020
   PubMed Web of Science ®Google Scholar
• Yang X, Dong N, Zhang R, Chen S. Carbapenem resistance-encoding and virulence-encoding conjugative plasmids in Klebsiella pneumoniae. Trends Microbiol. 2021;29(1):65–83. doi:10.1016/j.tim.2020.04.012
   PubMed Web of Science ®Google Scholar
• Zhang Y, Jin L, Ouyang P, et al. Evolution of hypervirulence in carbapenem-resistant Klebsiella pneumoniae in China: a multicentre, molecular epidemiological analysis. J Antimicrob Chemother. 2020;75(2):327–336. doi:10.1093/jac/dkz446
   PubMed Web of Science ®Google Scholar
• Xie Y, Tian L, Li G, et al. Emergence of the third-generation cephalosporin-resistant hypervirulent Klebsiella pneumoniae due to the acquisition of a self-transferable bladha-1-carrying plasmid by an st23 strain. Virulence. 2018;9(1):838–844.
   PubMed Web of Science ®Google Scholar
• Xie M, Yang X, Xu Q, et al. Clinical evolution of st11 carbapenem resistant and hypervirulent Klebsiella pneumoniae. Commun Biol. 2021;4(1):650. doi:10.1038/s42003-021-02148-4
   PubMed Web of Science ®Google Scholar
• Zhang R, Lin D, Gu D, Chen G, Chen S. Emergence of carbapenem-resistant serotype k1 hypervirulent Klebsiella pneumoniae strains in China. Antimicrob Agents Chemother. 2015;60(1):709–711. doi:10.1128/AAC.02173-15
   PubMed Web of Science ®Google Scholar
• Dong N, Lin D, Zhang R, Chan EW, Chen S. Carriage of blakpc-2 by a virulence plasmid in hypervirulent Klebsiella pneumoniae. J Antimicrob Chemother. 2018;73(12):3317–3321. doi:10.1093/jac/dky358
   PubMed Web of Science ®Google Scholar
• Huang YH, Chou SH, Liang SW, et al. Emergence of an xdr and carbapenemase-producing hypervirulent Klebsiella pneumoniae strain in Taiwan. J Antimicrob Chemother. 2018;73(8):2039–2046. doi:10.1093/jac/dky164
   PubMed Web of Science ®Google Scholar
• Lam MMC, Wyres KL, Wick RR, et al. Convergence of virulence and mdr in a single plasmid vector in mdr Klebsiella pneumoniae st15. J Antimicrob Chemother. 2019;74(5):1218–1222. doi:10.1093/jac/dkz028
   PubMed Web of Science ®Google Scholar
• Turton J, Davies F, Turton J, Perry C, Payne Z, Pike R. Hybrid resistance and virulence plasmids in “high-risk” clones of Klebsiella pneumoniae, including those carrying blandm-5. Microorganisms. 2019;7(9):1–11. doi:10.3390/microorganisms7090326
   Web of Science ®Google Scholar
• Zhao Y, Zhang X, Torres VVL, et al. An outbreak of carbapenem-resistant and hypervirulent Klebsiella pneumoniae in an intensive care unit of a major teaching hospital in Wenzhou, China. Front Public Health. 2019;7:229. doi:10.3389/fpubh.2019.00229
   PubMed Web of Science ®Google Scholar
• Serio AW, Keepers T, Andrews L, Krause KM. Aminoglycoside revival: review of a historically important class of antimicrobials undergoing rejuvenation. Ecosal Plus. 2018;8(1):1–20. doi:10.1128/ecosalplus.ESP-0002-2018
   Google Scholar
• Raut A, Sharma D, Suvarna V. A status update on pharmaceutical analytical methods of aminoglycoside antibiotic: amikacin. Crit Rev Anal Chem. 2022;52(2):375–391. doi:10.1080/10408347.2020.1803042
   PubMed Web of Science ®Google Scholar
• Yang W, Hu F. Research updates of plasmid-mediated aminoglycoside resistance 16s rRNA methyltransferase. Antibiotics. 2022;11(7). doi:10.3390/antibiotics11070906
   Google Scholar
• Wachino JI, Doi Y, Arakawa Y. Aminoglycoside resistance: updates with a focus on acquired 16s ribosomal RNA methyltransferases. Infect Dis Clin North Am. 2020;34(4):887–902. doi:10.1016/j.idc.2020.06.002
   PubMed Web of Science ®Google Scholar
• Kawai A, Suzuki M, Tsukamoto K, Minato Y, Doi Y. Functional and structural characterization of acquired 16s rRNA methyltransferase npmb1 conferring pan-aminoglycoside resistance. Antimicrob Agents Chemother. 2021;65(10):e100921. doi:10.1128/AAC.01009-21
   Web of Science ®Google Scholar
• Camelena F, Morel F, Merimeche M, et al. Genomic characterization of 16s rRNA methyltransferase-producing Escherichia coli isolates from the Parisian area, France. J Antimicrob Chemother. 2020;75(7):1726–1735. doi:10.1093/jac/dkaa105
   PubMed Web of Science ®Google Scholar
• Uchida H, Tada T, Tohya M, et al. Emergence in Japan of an isolate of Klebsiella pneumoniae co-harbouring bla(kpc-2) and rmtb. J Glob Antimicrob Resist. 2019;17:157–159. doi:10.1016/j.jgar.2018.11.026
   PubMed Web of Science ®Google Scholar
• Amin M, Mehdipour G, Navidifar T. High distribution of 16s rRNA methylase genes rmtb and arma among Enterobacter cloacae strains isolated from an ahvaz teaching hospital, Iran. Acta Microbiol Immunol Hung. 2019;66(3):337–348. doi:10.1556/030.66.2019.009
   PubMed Web of Science ®Google Scholar
• Nagasawa M, Kaku M, Kamachi K, et al. Loop-mediated isothermal amplification assay for 16s rRNA methylase genes in gram-negative bacteria. J Infect Chemother. 2014;20(10):635–638. doi:10.1016/j.jiac.2014.08.013
   PubMed Web of Science ®Google Scholar
• CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 31st ed. Wayne, PA: Clinical and Laboratory Standards Institute; 2021.
   Google Scholar
• Hua Y, Wang J, Huang M, et al. Outer membrane vesicles-transmitted virulence genes mediate the emergence of new antimicrobial-resistant hypervirulent Klebsiella pneumoniae. Emerg Microbes Infect. 2022;11(1):1281–1292. doi:10.1080/22221751.2022.2065935
   PubMed Web of Science ®Google Scholar
• Wick RR, Judd LM, Gorrie CL, Holt KE. Unicycler: resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol. 2017;13(6):e1005595. doi:10.1371/journal.pcbi.1005595
   PubMed Web of Science ®Google Scholar
• Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014;30(14):2068–2069. doi:10.1093/bioinformatics/btu153
   PubMed Web of Science ®Google Scholar
• Wang M, Goh YX, Tai C, Wang H, Deng Z, Ou HY. Vrprofile2: detection of antibiotic resistance-associated mobilome in bacterial pathogens. Nucleic Acids Res. 2022;50(W1):W768–773. doi:10.1093/nar/gkac321
   PubMed Web of Science ®Google Scholar
• Jin X, Chen Q, Shen F, et al. Resistance evolution of hypervirulent carbapenem-resistant Klebsiella pneumoniae st11 during treatment with tigecycline and polymyxin. Emerg Microbes Infect. 2021;10(1):1129–1136. doi:10.1080/22221751.2021.1937327
   PubMed Web of Science ®Google Scholar
• Alikhan NF, Petty NK, Ben ZN, Beatson SA. Blast ring image generator (brig): simple prokaryote genome comparisons. BMC Genomics. 2011;12:402. doi:10.1186/1471-2164-12-402
   PubMed Web of Science ®Google Scholar
• Sullivan MJ, Petty NK, Beatson SA. Easyfig: a genome comparison visualizer. Bioinformatics. 2011;27(7):1009–1010. doi:10.1093/bioinformatics/btr039
   PubMed Web of Science ®Google Scholar
• Wang X, Chen G, Wu X, et al. Increased prevalence of carbapenem resistant Enterobacteriaceae in hospital setting due to cross-species transmission of the bla ndm-1 element and clonal spread of progenitor resistant strains. Front Microbiol. 2015;6:595. doi:10.3389/fmicb.2015.00595
   PubMed Web of Science ®Google Scholar
• Tenover FC, Arbeit RD, Goering RV, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995;33(9):2233–2239. doi:10.1128/jcm.33.9.2233-2239.1995
   PubMed Web of Science ®Google Scholar
• Mukherjee S, Bhadury P, Mitra S, et al. Hypervirulent Klebsiella pneumoniae causing neonatal bloodstream infections: emergence of ndm-1-producing hypervirulent st11-k2 and st15-k54 strains possessing plvpk-associated markers. Microbiol Spectr. 2023;11(2):e412122. doi:10.1128/spectrum.04121-22
   Web of Science ®Google Scholar
• Semenec L, Cain AK, Dawson CJ, et al. Cross-protection and cross-feeding between Klebsiella pneumoniae and Acinetobacter baumannii promotes their co-existence. Nat Commun. 2023;14(1):702. doi:10.1038/s41467-023-36252-2
   PubMed Web of Science ®Google Scholar
• Tian D, Wang W, Li M, et al. Acquisition of the conjugative virulence plasmid from a cg23 hypervirulent Klebsiella pneumoniae strain enhances bacterial virulence. Front Cell Infect Microbiol. 2021;11:752011. doi:10.3389/fcimb.2021.752011
   PubMed Web of Science ®Google Scholar
• Wang L, Zhang Y, Liu Y, et al. Effects of chlorogenic acid on antimicrobial, antivirulence, and anti-quorum sensing of carbapenem-resistant Klebsiella pneumoniae. Front Microbiol. 2022;13:997310. doi:10.3389/fmicb.2022.997310
   PubMed Web of Science ®Google Scholar
• Loyola IH, Brito IL. Characterizing conjugative plasmids from an antibiotic-resistant dataset for use as broad-host delivery vectors. Front Microbiol. 2023;14:1199640. doi:10.3389/fmicb.2023.1199640
   PubMed Web of Science ®Google Scholar
• Lai YC, Peng HL, Chang HY. Rmpa2, an activator of capsule biosynthesis in Klebsiella pneumoniae cg43, regulates k2 cps gene expression at the transcriptional level. J Bacteriol. 2003;185(3):788–800. doi:10.1128/JB.185.3.788-800.2003
   PubMed Web of Science ®Google Scholar
• Zhou K, Xiao T, David S, et al. Novel subclone of carbapenem-resistant Klebsiella pneumoniae sequence type 11 with enhanced virulence and transmissibility, China. Emerg Infect Dis. 2020;26(2):289–297. doi:10.3201/eid2602.190594
   PubMed Web of Science ®Google Scholar
• Zhou C, Wu Q, He L, et al. Clinical and molecular characteristics of carbapenem-resistant hypervirulent Klebsiella pneumoniae isolates in a tertiary hospital in shanghai, China. Infect Drug Resist. 2021;14:2697–2706. doi:10.2147/IDR.S321704
   PubMed Web of Science ®Google Scholar
• Dong N, Yang X, Zhang R, Chan EW, Chen S. Tracking microevolution events among st11 carbapenemase-producing hypervirulent Klebsiella pneumoniae outbreak strains. Emerg Microbes Infect. 2018;7(1):146. doi:10.1038/s41426-018-0146-6
   PubMed Web of Science ®Google Scholar
• Chen Y, Marimuthu K, Teo J, et al. Acquisition of plasmid with carbapenem-resistance gene blakpc2 in hypervirulent Klebsiella pneumoniae, Singapore. Emerg Infect Dis. 2020;26(3):549–559. doi:10.3201/eid2603.191230
   PubMed Web of Science ®Google Scholar
• Yu FY, Yao D, Pan JY, et al. High prevalence of plasmid-mediated 16s rRNA methylase gene rmtb among Escherichia coli clinical isolates from a Chinese teaching hospital. BMC Infect Dis. 2010;10:184. doi:10.1186/1471-2334-10-184
   PubMed Web of Science ®Google Scholar
• Chen Y, Chang H, Lai Y, Pan C, Tsai S, Peng H. Sequencing and analysis of the large virulence plasmid plvpk of Klebsiella pneumoniae cg43. Gene. 2004;337:189–198. doi:10.1016/j.gene.2004.05.008
   PubMed Web of Science ®Google Scholar
• Zhou Y, Ai W, Guo Y, et al. Co-occurrence of rare arma-, rmtb-, and kpc-2-encoding multidrug-resistant plasmids and hypervirulence iuc operon in st11-kl47 Klebsiella pneumoniae. Microbiol Spectr. 2022;10(2):e237121. doi:10.1128/spectrum.02371-21
   Web of Science ®Google Scholar
• Xia P, Yi M, Yuan Y, et al. Coexistence of multidrug resistance and virulence in a single conjugative plasmid from a hypervirulent Klebsiella pneumoniae isolate of sequence type 25. Msphere. 2022;7(6):e47722. doi:10.1128/msphere.00477-22
   PubMed Web of Science ®Google Scholar
• Jia X, Zhu Y, Jia P, et al. Emergence of a superplasmid coharboring hypervirulence and multidrug resistance genes in Klebsiella pneumoniae poses new challenges to public health. Microbiol Spectr. 2022;10(6):e263422. doi:10.1128/spectrum.02634-22
   Web of Science ®Google Scholar
• Yang X, Wai-Chi CE, Zhang R, Chen S. A conjugative plasmid that augments virulence in Klebsiella pneumoniae. Nat Microbiol. 2019;4(12):2039–2043. doi:10.1038/s41564-019-0566-7
   PubMed Web of Science ®Google Scholar
• Shon AS, Bajwa RP, Russo TA. Hypervirulent (hypermucoviscous) Klebsiella pneumoniae: a new and dangerous breed. Virulence. 2013;4(2):107–118. doi:10.4161/viru.22718
   PubMed Web of Science ®Google Scholar
• Choby JE, Howard-Anderson J, Weiss DS. Hypervirulent Klebsiella pneumoniae - clinical and molecular perspectives. J Intern Med. 2020;287(3):283–300. doi:10.1111/joim.13007
   PubMed Web of Science ®Google Scholar
• Long D, Zhu L, Du F, et al. Phenotypical profile and global transcriptomic profile of hypervirulent Klebsiella pneumoniae due to carbapenemase-encoding plasmid acquisition. BMC Genomics. 2019;20(1):480. doi:10.1186/s12864-019-5705-2
   PubMedGoogle Scholar
• Choi MJ, Ko KS. Loss of hypermucoviscosity and increased fitness cost in colistin-resistant Klebsiella pneumoniae sequence type 23 strains. Antimicrob Agents Chemother. 2015;59(11):6763–6773. doi:10.1128/AAC.00952-15
   PubMed Web of Science ®Google Scholar
• Zhang Y, Wang X, Wang S, et al. Emergence of colistin resistance in carbapenem-resistant hypervirulent Klebsiella pneumoniae under the pressure of tigecycline. Front Microbiol. 2021;12:756580. doi:10.3389/fmicb.2021.756580
   PubMed Web of Science ®Google Scholar
• Fursova AD, Fursov MV, Astashkin EI, et al. Early response of antimicrobial resistance and virulence genes expression in classical, hypervirulent, and hybrid hvkp-mdr Klebsiella pneumoniae on antimicrobial stress. Antibiotics. 2022;11(1):7. doi:10.3390/antibiotics11010007
   Google Scholar
• Pu D, Zhao J, Lu B, et al. Within-host resistance evolution of a fatal st11 hypervirulent carbapenem-resistant Klebsiella pneumoniae. Int J Antimicrob Agents. 2023;61(4):106747. doi:10.1016/j.ijantimicag.2023.106747
   PubMed Web of Science ®Google Scholar
• Zhang R, Liu L, Zhou H, et al. Nationwide surveillance of clinical carbapenem-resistant Enterobacteriaceae (cre) strains in China. Ebiomedicine. 2017;19:98–106. doi:10.1016/j.ebiom.2017.04.032
   PubMed Web of Science ®Google Scholar
• Russo TA, Marr CM. Hypervirulent Klebsiella pneumoniae. Clin Microbiol Rev. 2019;32(3):e00001–19. doi:10.1128/CMR.00001-19
   PubMed Web of Science ®Google Scholar
• Wyres KL, Wick RR, Judd LM, et al. Distinct evolutionary dynamics of horizontal gene transfer in drug resistant and virulent clones of Klebsiella pneumoniae. PLoS Genet. 2019;15(4):e1008114. doi:10.1371/journal.pgen.1008114
   PubMed Web of Science ®Google Scholar