Evaluating the use of self-conditioning adhesive combined with dual curing resin cement as an endodontic sealer: An in vitro study.

Abstract

Objectives

To compare infiltration, sealing and microleakage in root dentin with a self-conditioning adhesive system combined with dual curing resin (resin-based cement) to a conventional epoxy-resin-based sealer using confocal microscopy imaging.

Methods

26 roots were enlarged and disinfected. Dentin tubules of 24 teeth were labelled with a red fluorophore (Rhodamine B) (two samples served as controls). Root canal samples were sealed in group AH (n = 11) with a conventional sealer (AH Plus Root Canal Sealer, Dentsply DeTrey) and in group RC (n = 11) with a resin-based cement (Parabond combined with Paracore, Coltène). Roots were then sectioned horizontally and immersed in H₂O₂ to remove the Rhodamine B not fixed by the sealers. The empty dentin spaces were labeled with a green fluorophore (Fluorescein) enabling the evaluation of infiltration as well as microleakage by confocal microscopy. Two additional samples were fractured in vertical direction for observation under SEM.

Results

Group RC presented significantly more infiltration in the middle third than in the middle and apical thirds of group AH. Microleakage was significantly higher in group AH than in group RC. SEM images revealed more dentin plugs and a homogenous resin layer in group RC in contrast to group AH.

Conclusion

The resin-based cement revealed promising outcomes compared to a traditional epoxy resin based sealer.

Key messages

Infiltration and microleakage in infiltrated and sealed root dentin samples are higher in middle than apical root thirds.

Root dentin infiltration and sealing with a self-conditioning adhesive system and a dual-curing resin cement revealed less microleakage than with an epoxy-resin-based sealer.

Keywords:

• composite resin
• sealer
• adhesion
• cement
• infiltration
• microleakage
• confocal microscopy
• endodontics
• dentin

Acknowledgements

Isaline Rossier, Luciana Caseiro, Bioimaging facility (UNIGE), Olivier Brun et François Prodon

Author contribution

Conception and design (CIA, IK, LM, NL); Analysis and interpretation of data (CIA, IK, LM, EDB); Drafting of the paper (CIA, LM, EDB); Revising paper critically for intellectual content (AF, MA); final approval of version to be published (all authors agree to be accountable for all aspects of the work)

Disclosure statement

The authors report no conflict of interest

Data availability

The data that support the findings of this study are available from the corresponding author, [CIA], upon request.