365
Views
61
CrossRef citations to date
0
Altmetric
Research Article

Cutaneous DNA delivery and gene expression in ex vivo human skin explants via wet-etch microfabricated microneedles

, , , , , , , & show all
Pages 415-421 | Received 09 Sep 2005, Accepted 22 Sep 2005, Published online: 08 Oct 2008

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (8)

Thuy Trang Nguyen & Jung Hwan Park. (2018) Human studies with microneedles for evaluation of their efficacy and safety. Expert Opinion on Drug Delivery 15:3, pages 235-245.
Read now
Harvinder S Gill, Sang-Moo Kang, Fu-Shi Quan & Richard W Compans. (2014) Cutaneous immunization: an evolving paradigm in influenza vaccines. Expert Opinion on Drug Delivery 11:4, pages 615-627.
Read now
Sang-Moo Kang, Jae-Min Song & Yeu-Chun Kim. (2012) Microneedle and mucosal delivery of influenza vaccines. Expert Review of Vaccines 11:5, pages 547-560.
Read now
R. B. Groves, S. A. Coulman, J. C. Birchall & S. L. Evans. (2012) Quantifying the mechanical properties of human skin to optimise future microneedle device design. Computer Methods in Biomechanics and Biomedical Engineering 15:1, pages 73-82.
Read now
Shaun D Gittard, Aleksandr Ovsianikov, Boris N Chichkov, Anand Doraiswamy & Roger J Narayan. (2010) Two-photon polymerization of microneedles for transdermal drug delivery. Expert Opinion on Drug Delivery 7:4, pages 513-533.
Read now
Joost H. van den Berg, Bastiaan Nuijen, Ton N. Schumacher, John B.A.G. Haanen, Gert Storm, Jos H. Beijnen & Wim E. Hennink. (2010) Synthetic vehicles for DNA vaccination. Journal of Drug Targeting 18:1, pages 1-14.
Read now
Raja K Sivamani, Dorian Liepmann & Howard I Maibach. (2007) Microneedles and transdermal applications. Expert Opinion on Drug Delivery 4:1, pages 19-25.
Read now
James C Birchall. (2006) Microneedle array technology: the time is right but is the science ready?. Expert Review of Medical Devices 3:1, pages 1-4.
Read now

Articles from other publishers (53)

Chukwuebuka Emmanuel Umeyor, Varsha Shelke, Apurva Pol, Prafull Kolekar, Sarika Jadhav, Nikita Tiwari, Ankita Anure, Abhit Nayak, Ghanshamdas Bairagi, Aniket Agale, Vaishnavi Raut, Sumedh Bahadure, Aniruddha Chaudhari & Vandana B. Patravale. (2023) Biomimetic microneedles: exploring the recent advances on a microfabricated system for precision delivery of drugs, peptides, and proteins. Future Journal of Pharmaceutical Sciences 9:1.
Crossref
Han Bi Kim, Min Gyu Choi, Bo Young Chung, Ji Young Um, Jin Cheol Kim, Chun Wook Park & Hye One Kim. (2022) Particulate matter 2.5 induces the skin barrier dysfunction and cutaneous inflammation via AhR ‐ and T helper 17 cell‐related genes in human skin tissue as identified via transcriptome analysis . Experimental Dermatology 32:4, pages 547-554.
Crossref
Inhwan Noh, Kyuri Lee & Yun-Seok Rhee. (2022) Microneedle systems for delivering nucleic acid drugs. Journal of Pharmaceutical Investigation 52:3, pages 273-292.
Crossref
Defu Zhi, Ting Yang, Tingyu Zhang, Mei Yang, Shubiao Zhang & Ryan F. Donnelly. (2021) Microneedles for gene and drug delivery in skin cancer therapy. Journal of Controlled Release 335, pages 158-177.
Crossref
Lalit K. Vora, Kurtis Moffatt, Ismaiel A. Tekko, Alejandro J. Paredes, Fabiana Volpe-Zanutto, Deepakkumar Mishra, Ke Peng, Raghu Raj Singh Thakur & Ryan F. Donnelly. (2021) Microneedle array systems for long-acting drug delivery. European Journal of Pharmaceutics and Biopharmaceutics 159, pages 44-76.
Crossref
Joseph G. Turner, Leah R. White, Pedro Estrela & Hannah S. Leese. (2020) Hydrogel‐Forming Microneedles: Current Advancements and Future Trends. Macromolecular Bioscience 21:2, pages 2000307.
Crossref
Ashana Puri, Hiep X Nguyen, Akeemat O Tijani & Ajay K Banga. (2021) Characterization of microneedles and microchannels for enhanced transdermal drug delivery. Therapeutic Delivery 12:1, pages 77-103.
Crossref
Kurtis Moffatt & Ryan F. Donnelly. 2021. Drug Delivery Devices and Therapeutic Systems. Drug Delivery Devices and Therapeutic Systems 345 366 .
I. Bauhammer, M. Sacha & E. Haltner. (2019) Establishment of a novel in vitro viable human skin model as a basis for the treatment of human and veterinary chronic skin diseases. Journal of Drug Delivery Science and Technology 51, pages 695-699.
Crossref
I. Bauhammer, M. Sacha & E. Haltner. (2019) Validation and stability analysis of a modified lactate dehydrogenase (LDH) test method to be employed for an in vitro viable skin model. Heliyon 5:5, pages e01618.
Crossref
Lipika Chablani, Suprita A. Tawde, Archana Akalkotkar & Martin J. D’Souza. (2019) Evaluation of a Particulate Breast Cancer Vaccine Delivered via Skin. The AAPS Journal 21:2.
Crossref
Grace Cole, Nicholas J. Dunne & Helen O. McCarthy. 2018. Microneedles for Drug and Vaccine Delivery and Patient Monitoring. Microneedles for Drug and Vaccine Delivery and Patient Monitoring 129 175 .
M Dul, M Stefanidou, P Porta, J Serve, C O'Mahony, B Malissen, S Henri, Y Levin, E Kochba, FS Wong, C Dayan, SA Coulman & JC Birchall. (2017) Hydrodynamic gene delivery in human skin using a hollow microneedle device. Journal of Controlled Release 265, pages 120-131.
Crossref
K. Moronkeji, S. Todd, I. Dawidowska, S.D. Barrett & R. Akhtar. (2017) The role of subcutaneous tissue stiffness on microneedle performance in a representative in vitro model of skin. Journal of Controlled Release 265, pages 102-112.
Crossref
Ryan Donnelly, Maelíosa McCrudden, Sharifa Al-Zahrani & Steven Fallows. 2017. Drug Delivery. Drug Delivery 207 236 .
Amit Kumar, Yuehong Xu & Zhengrong Cui. 2017. Percutaneous Penetration Enhancers Physical Methods in Penetration Enhancement. Percutaneous Penetration Enhancers Physical Methods in Penetration Enhancement 441 462 .
S.H.T. Jorritsma, E.J. Gowans, B. Grubor-Bauk & D.K. Wijesundara. (2016) Delivery methods to increase cellular uptake and immunogenicity of DNA vaccines. Vaccine 34:46, pages 5488-5494.
Crossref
Marianna Foldvari, Ding Wen Chen, Nafiseh Nafissi, Daniella Calderon, Lokesh Narsineni & Amirreza Rafiee. (2016) Non-viral gene therapy: Gains and challenges of non-invasive administration methods. Journal of Controlled Release 240, pages 165-190.
Crossref
Wei Chen, Hui Li, De Shi, Zhenguo Liu & Weien Yuan. (2016) Microneedles As a Delivery System for Gene Therapy. Frontiers in Pharmacology 7.
Crossref
Regina Luttge. 2016. Nano- and Microfabrication for Industrial and Biomedical Applications. Nano- and Microfabrication for Industrial and Biomedical Applications 215 248 .
Matthias GieseMatthias Giese. 2016. Introduction to Molecular Vaccinology. Introduction to Molecular Vaccinology 233 258 .
Joanne McCaffrey, Ryan F. Donnelly & Helen O. McCarthy. (2015) Microneedles: an innovative platform for gene delivery. Drug Delivery and Translational Research 5:4, pages 424-437.
Crossref
Rebecca E. M. Lutton, Jessica Moore, Eneko Larrañeta, Stephen Ligett, A. David Woolfson & Ryan F. Donnelly. (2015) Microneedle characterisation: the need for universal acceptance criteria and GMP specifications when moving towards commercialisation. Drug Delivery and Translational Research 5:4, pages 313-331.
Crossref
M. S. Giri Nandagopal, Rahul Antony, S. Rangabhashiyam, Nidhin Sreekumar & N. Selvaraju. (2014) Overview of microneedle system: a third generation transdermal drug delivery approach. Microsystem Technologies 20:7, pages 1249-1272.
Crossref
Ryan F. Donnelly, Sharifa Al-Zahrani, Marija Zaric, Cian M. McCrudden, Cristopher J. Scott & Adrien Kissenpfenning. 2014. Molecular Vaccines. Molecular Vaccines 697 715 .
Yusuf K. Demir, Zafer Akan & Oya Kerimoglu. (2013) Characterization of Polymeric Microneedle Arrays for Transdermal Drug Delivery. PLoS ONE 8:10, pages e77289.
Crossref
Mikolaj Milewski, Kalpana S. Paudel, Nicole K. Brogden, Priyanka Ghosh, Stan L. Banks, Dana C. Hammell & Audra L. Stinchcomb. (2013) Microneedle-Assisted Percutaneous Delivery of Naltrexone Hydrochloride in Yucatan Minipig: In Vitro–In Vivo Correlation. Molecular Pharmaceutics 10:10, pages 3745-3757.
Crossref
Rosalind H.E. Chong, Emilio Gonzalez-Gonzalez, Maria F. Lara, Tycho J. Speaker, Christopher H. Contag, Roger L. Kaspar, Sion A. Coulman, Rachel Hargest & James C. Birchall. (2013) Gene silencing following siRNA delivery to skin via coated steel microneedles: In vitro and in vivo proof-of-concept. Journal of Controlled Release 166:3, pages 211-219.
Crossref
B.M. Torrisi, V. Zarnitsyn, M.R. Prausnitz, A. Anstey, C. Gateley, J.C. Birchall & S.A. Coulman. (2013) Pocketed microneedles for rapid delivery of a liquid-state botulinum toxin A formulation into human skin. Journal of Controlled Release 165:2, pages 146-152.
Crossref
Yusuf K. Demir, Zafer Akan & Oya Kerimoglu. (2012) WITHDRAWN: Microfabrication of dissolvable, swellable, and biodegradable polymeric microneedle arrays. International Journal of Pharmaceutics.
Crossref
Marc Pearton, Verena Saller, Sion A. Coulman, Chris Gateley, Alexander V. Anstey, Vladimir Zarnitsyn & James C. Birchall. (2012) Microneedle delivery of plasmid DNA to living human skin: Formulation coating, skin insertion and gene expression. Journal of Controlled Release 160:3, pages 561-569.
Crossref
James Birchall. (2012) Interview: Mini Focus: Interview with Dr James Birchall. Therapeutic Delivery 3:3, pages 311-313.
Crossref
Ryan D Boehm, Philip R Miller, Ritika Singh, Akash Shah, Shane Stafslien, Justin Daniels & Roger J Narayan. (2012) Indirect rapid prototyping of antibacterial acid anhydride copolymer microneedles. Biofabrication 4:1, pages 011002.
Crossref
Ryan F. Donnelly, Thakur Raghu Raj Singh, Desmond I. J. Morrow & A. David Woolfson. 2012. Microneedle‐Mediated Transdermal and Intradermal Drug Delivery. Microneedle‐Mediated Transdermal and Intradermal Drug Delivery 113 151 .
Emilio González-González, Yeu-Chun Kim, Tycho J. Speaker, Robyn P. Hickerson, Ryan Spitler, James C. Birchall, Maria Fernanda Lara, Rong-hua Hu, Yanhua Liang, Nancy Kirkiles-Smith, Mark R. Prausnitz, Leonard M. Milstone, Christopher H. Contag & Roger L. Kaspar. (2011) Visualization of plasmid delivery to keratinocytes in mouse and human epidermis. Scientific Reports 1:1.
Crossref
R D Boehm, P R Miller, S L Hayes, N A Monteiro-Riviere & R J Narayan. (2011) Modification of microneedles using inkjet printing. AIP Advances 1:2.
Crossref
Regina Luttge. 2011. Microfabrication for Industrial Applications. Microfabrication for Industrial Applications 235 272 .
Siôn A. Coulman, James C. Birchall, Aneesh Alex, Marc Pearton, Bernd Hofer, Conor O’Mahony, Wolfgang Drexler & Boris Považay. (2010) In Vivo, In Situ Imaging of Microneedle Insertion into the Skin of Human Volunteers Using Optical Coherence Tomography. Pharmaceutical Research 28:1, pages 66-81.
Crossref
James C. Birchall, Rachel Clemo, Alexander Anstey & Dai N. John. (2010) Microneedles in Clinical Practice–An Exploratory Study Into the Opinions of Healthcare Professionals and the Public. Pharmaceutical Research 28:1, pages 95-106.
Crossref
Jae-Min Song, Yeu-Chun Kim, Aleksandr S. Lipatov, Marc Pearton, C. Todd Davis, Dae-Goon Yoo, Kyoung-Mi Park, Li-Mei Chen, Fu-Shi Quan, James C. Birchall, Ruben O. Donis, Mark R. Prausnitz, Richard W. Compans & Sang-Moo Kang. (2010) Microneedle Delivery of H5N1 Influenza Virus-Like Particles to the Skin Induces Long-Lasting B- and T-Cell Responses in Mice. Clinical and Vaccine Immunology 17:9, pages 1381-1389.
Crossref
Emilio Gonzalez-Gonzalez, Tycho J Speaker, Robyn P Hickerson, Ryan Spitler, Manuel A Flores, Devin Leake, Christopher H Contag & Roger L Kaspar. (2010) Silencing of Reporter Gene Expression in Skin Using siRNAs and Expression of Plasmid DNA Delivered by a Soluble Protrusion Array Device (PAD). Molecular Therapy 18:9, pages 1667-1674.
Crossref
Marc Pearton, Sang-Moo Kang, Jae-Min Song, Alexander V. Anstey, Matthew Ivory, Richard W. Compans & James C. Birchall. (2010) Changes in Human Langerhans Cells Following Intradermal Injection of Influenza Virus-Like Particle Vaccines. PLoS ONE 5:8, pages e12410.
Crossref
Marc Pearton, Sang-Moo Kang, Jae-Min Song, Yeu-Chun Kim, Fu-Shi Quan, Alexander Anstey, Matthew Ivory, Mark R. Prausnitz, Richard W. Compans & James C. Birchall. (2010) Influenza virus-like particles coated onto microneedles can elicit stimulatory effects on Langerhans cells in human skin. Vaccine 28:37, pages 6104-6113.
Crossref
K. Oosterhuis, J. H. van den Berg, T. N. Schumacher & J. B. A. G. Haanen. 2012. Intradermal Immunization. Intradermal Immunization 221 250 .
Keng Wooi Ng, Marc Pearton, Sion Coulman, Alexander Anstey, Christopher Gateley, Anthony Morrissey, Christopher Allender & James Birchall. (2009) Development of an ex vivo human skin model for intradermal vaccination: Tissue viability and Langerhans cell behaviour. Vaccine 27:43, pages 5948-5955.
Crossref
M. I. Haq, E. Smith, D. N. John, M. Kalavala, C. Edwards, A. Anstey, A. Morrissey & J. C. Birchall. (2008) Clinical administration of microneedles: skin puncture, pain and sensation. Biomedical Microdevices 11:1, pages 35-47.
Crossref
Sion A. Coulman, Alexander Anstey, Chris Gateley, Anthony Morrissey, Peter McLoughlin, Chris Allender & James C. Birchall. (2009) Microneedle mediated delivery of nanoparticles into human skin. International Journal of Pharmaceutics 366:1-2, pages 190-200.
Crossref
Mark R. Prausnitz, John A. Mikszta, Michel Cormier & Alexander K. Andrianov. 2009. Vaccines for Pandemic Influenza. Vaccines for Pandemic Influenza 369 393 .
Mark Prausnitz, Harvinder Gill & Jung-Hwan Park. 2008. Modified-Release Drug Delivery Technology, Second Edition. Modified-Release Drug Delivery Technology, Second Edition 295 309 .
Marc Pearton, Chris Allender, Keith Brain, Alexander Anstey, Chris Gateley, Nicolle Wilke, Anthony Morrissey & James Birchall. (2007) Gene Delivery to the Epidermal Cells of Human Skin Explants Using Microfabricated Microneedles and Hydrogel Formulations. Pharmaceutical Research 25:2, pages 407-416.
Crossref
James Birchall & Keith Brain. 2007. Dermatologic, Cosmeceutic, and Cosmetic Development. Dermatologic, Cosmeceutic, and Cosmetic Development 577 589 .
Ga?lle Vandermeulen, Li?vin Daugimont & V?ronique Pr?at. 2007. Dermatologic, Cosmeceutic, and Cosmetic Development. Dermatologic, Cosmeceutic, and Cosmetic Development 537 555 .
Daisuke Yoshida, Hiroaki Todo, Tetsuya Hasegawa & Kenji Sugibayashi. (2007) Dermatopharmacokinetics of salicylate following topical injection in rats: Effect of osmotic pressure and injection volume on salicylate disposition. International Journal of Pharmaceutics 337:1-2, pages 142-147.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.