Investigation of biological effects of HEMA in 3D-organotypic co-culture models of normal and malignant oral keratinocytes

References

• Reichl F-X, Löhle J, Seiss M, et al. Elution of TEGDMA and HEMA from polymerized resin-based bonding systems. Dent Mater. 2012; 28(11):1120–1125. doi: 10.1016/j.dental.2012.06.010.
   PubMed Web of Science ®Google Scholar
• Van Landuyt KL, Nawrot T, Geebelen B, et al. How much do resin-based dental materials release? A meta-analytical approach. Dent Mater. 2011; 27(8):723–747. doi: 10.1016/j.dental.2011.05.001.
   PubMed Web of Science ®Google Scholar
• Samuelsen JT, Dahl JE, Karlsson S, et al. Apoptosis induced by the monomers HEMA and TEGDMA involves formation of ROS and differential activation of the MAP-kinases p38, JNK and ERK. Dent Mater. 2007;23(1):34–39. doi: 10.1016/j.dental.2005.11.037.
   PubMed Web of Science ®Google Scholar
• Teti G, Orsini G, Salvatore V, et al. HEMA but not TEGDMA induces autophagy in human gingival fibroblasts. Front Physiol. 2015;6:1–8. doi: 10.3389/fphys.2015.00275.
   PubMed Web of Science ®Google Scholar
• Samuelsen JT, Holme JA, Becher R, et al. HEMA reduces cell proliferation and induces apoptosis in vitro. Dent Mater. 2008;24(1):134–140. doi: 10.1016/j.dental.2007.08.006.
   PubMed Web of Science ®Google Scholar
• Perduns R, Volk J, Schertl P, et al. HEMA modulates the transcription of genes related to oxidative defense, inflammatory response and organization of the ECM in human oral cells. Dent Mater. 2019;35(3):501–510. doi: 10.1016/j.dental.2019.01.011.
   PubMed Web of Science ®Google Scholar
• Samuelsen JT, Kopperud HM, Holme JA, et al. Role of thiol-complex formation in 2-hydroxyethyl- methacrylate-induced toxicity in vitro. J Biomed Mater Res A. 2011;96(2):395–401. doi: 10.1002/jbm.a.32993.
   PubMedGoogle Scholar
• Krifka S, Petzel C, Hiller K-A, et al. Resin monomer-induced differential activation of MAP kinases and apoptosis in mouse macrophages and human pulp cells. Biomaterials. 2010;31(11):2964–2975. doi: 10.1016/j.biomaterials.2010.01.005.
   PubMed Web of Science ®Google Scholar
• Morisbak E, Ansteinsson V, Samuelsen JT. Cell toxicity of 2-hydroxyethyl methacrylate (HEMA): the role of oxidative stress. Eur J Oral Sci. 2015;123(4):282–287. doi: 10.1111/eos.12189.
   PubMed Web of Science ®Google Scholar
• Orimoto A, Suzuki T, Ueno A, et al. Effect of 2-hydroxyethyl methacrylate on antioxidant responsive element-mediated transcription: a possible indication of its cytotoxicity. PLoS One. 2013;8(3):e58907. doi: 10.1371/journal.pone.0058907.
   PubMed Web of Science ®Google Scholar
• Itoh K, Chiba T, Takahashi S, et al. An Nrf2/small maf heterodimer mediates the induction of phase II detoxifying enzyme genes through antioxidant response elements. Biochem Biophys Res Commun. 1997;236(2):313–322. doi: 10.1006/bbrc.1997.6943.
   PubMed Web of Science ®Google Scholar
• Gallorini M, Petzel C, Bolay C, et al. Activation of the Nrf2-regulated antioxidant cell response inhibits HEMA-induced oxidative stress and supports cell viability. Biomaterials. 2015;56:114–128. doi: 10.1016/j.biomaterials.2015.03.047.
   PubMed Web of Science ®Google Scholar
• Becher R, Valen H, Olderbø BP, et al. The dental monomer 2-hydroxyethyl methacrylate (HEMA) causes transcriptionally regulated adaptation partially initiated by electrophilic stress. Dent Mater. 2019;35(1):125–134. doi: 10.1016/j.dental.2018.11.008.
   PubMed Web of Science ®Google Scholar
• Wallenhammar L-M, Örtengren U, Andreasson H, et al. Contact allergy and hand eczema in Swedish dentists. Contact Dermatitis. 2000;43(4):192–199. doi: 10.1034/j.1600-0536.2000.043004192.x.
   PubMed Web of Science ®Google Scholar
• Hagberg S, Ljungkvist G, Andreasson H, et al. Exposure to volatile methacrylates in dental personnel. J Occup Environ Hyg. 2005;2(6):302–306. doi: 10.1080/15459620590958732.
   PubMed Web of Science ®Google Scholar
• Vintermyr OK, Costea DE, Loro LL, et al. Crucial effects of fibroblasts and keratinocyte growth factor on morphogenesis of reconstituted human oral epithelium. J Invest Dermatol. 2003;121(6):1479–1486. doi: 10.1111/j.1523-1747.2003.12616.x.
   PubMed Web of Science ®Google Scholar
• Cukierman E, Pankov R, Stevens DR, et al. Taking cell-matrix adhesions to the third dimension. Science. 2001;294(5547):1708–1712. doi: 10.1126/science.1064829.
   PubMed Web of Science ®Google Scholar
• Hillmann G, Gebert A, Geurtsen W. Matrix expression and proliferation of primary gingival fibroblasts in a three-dimensional cell culture model. J Cell Sci. 1999;112(17):2823–2832. doi: 10.1242/jcs.112.17.2823.
   PubMed Web of Science ®Google Scholar
• Klausner M, Handa Y, Aizawa S. In vitro three-dimensional organotypic culture models of the oral mucosa. In Vitro Cell Dev Biol Anim. 2021;57(2):148–159. doi: 10.1007/s11626-020-00539-1.
   PubMed Web of Science ®Google Scholar
• Perduns R, Volk J, Plum M, et al. Effects of HEMA on Nrf2-related gene expression using a newly developed 3D co-culture model of the oral mucosa. Dent Mater. 2019;35(9):1214–1226. doi: 10.1016/j.dental.2019.05.006.
   PubMed Web of Science ®Google Scholar
• Costea DE, Kulasekara K, Neppelberg E, et al. Species-specific fibroblasts required for triggering invasiveness of partially transformed oral keratinocytes. Am J Pathol. 2006;168(6):1889–1897. doi: 10.2353/ajpath.2006.050843.
   PubMed Web of Science ®Google Scholar
• Sapkota D, Bruland O, Parajuli H, et al. S100A16 promotes differentiation and contributes to a less aggressive tumor phenotype in oral squamous cell carcinoma. BMC Cancer. 2015;15(1):631. doi: 10.1186/s12885-015-1622-1.
   PubMedGoogle Scholar
• Costea DE, Dimba AOE, Loro LL, et al. The phenotype of in vitro reconstituted normal human oral epithelium is essentially determined by culture medium. J Oral Pathol Med. 2005;34(4):247–252. doi: 10.1111/j.1600-0714.2005.00308.x.
   PubMed Web of Science ®Google Scholar
• Schreurs O, Karatsaidis A, Balta MG, et al. Expression of keratins 8, 18, and 19 in epithelia of atrophic oral lichen planus. Eur J Oral Sci. 2020;128(1):7–17. doi: 10.1111/eos.12666.
   PubMed Web of Science ®Google Scholar
• Harper LJ, Piper K, Common J, et al. Stem cell patterns in cell lines derived from head and neck squamous cell carcinoma. J Oral Pathol Med. 2007;36(10):594–603. doi: 10.1111/j.1600-0714.2007.00617.x
   PubMed Web of Science ®Google Scholar
•  Rheinwald JG, Beckett MA. Tumorigenic keratino-cyte lines requiring anchorage and fibroblast support cultured from human squamous cell carcinomas. Cancer Res. 1981;41(5):1657–1663.
   Google Scholar
• Kusmardi K, Elvan W, Ari E, et al. Potential of Phaleria macrocarpa leaves ethanol extract to upregulate the expression of caspase-3 in mouse distal Colon after dextran sodium sulphate induction. PJ. 2021;13(1):23–29. doi: 10.5530/pj.2021.13.4.
   Google Scholar
• Diomede F, Tripodi D, Trubiani O, et al. HEMA effects on autophagy mechanism in human dental pulp stem cells. Materials. 2019;12(14):2285. doi: 10.3390/ma12142285.
   PubMed Web of Science ®Google Scholar
• Reichl FX, Walther UI, Durner J, et al. Cytotoxicity of dental composite components and mercury compounds in lung cells. Dent Mater. 2001;17(2):95–101. doi: 10.1016/s0109-5641(00)00029-4.
   PubMed Web of Science ®Google Scholar
• Ansteinsson V, Solhaug A, Samuelsen JT, et al. DNA-damage, cell-cycle arrest and apoptosis induced in BEAS-2B cells by 2-hydroxyethyl methacrylate (HEMA). Mutat Res. 2011;723(2):158–164. doi: 10.1016/j.mrgentox.2011.04.011.
   PubMed Web of Science ®Google Scholar
• Pawlowska E, Poplawski T, Ksiazek D, et al. Genotoxicity and cytotoxicity of 2-hydroxyethyl methacrylate. Mutat Res. 2010;696(2):122–129. doi: 10.1016/j.mrgentox.2009.12.019.
   PubMed Web of Science ®Google Scholar
• Schweikl H, Hartmann A, Hiller KA, et al. Inhibition of TEGDMA and HEMA-induced genotoxicity and cell cycle arrest by N-acetylcysteine. Dent Mater. 2007;23(6):688–695. doi: 10.1016/j.dental.2006.06.021.
   PubMed Web of Science ®Google Scholar
• Yoshii SR, Mizushima N. Monitoring and measuring autophagy. IJMS. 2017;18(9):1865. doi: 10.3390/ijms18091865.
   Web of Science ®Google Scholar
• Ly KL, Rooholghodos SA, Rahimi C, et al. An oral-mucosa-on-a-chip sensitively evaluates cell responses to dental monomers. Biomed Microdevices. 2021;23(1):7. doi: 10.1007/s10544-021-00543-6.
   PubMed Web of Science ®Google Scholar
• Rahimi C, Rahimi B, Padova D, et al. Oral mucosa-on-a-chip to assess layer-specific responses to bacteria and dental materials. Biomicrofluidics. 2018;12(5):054106. doi: 10.1063/1.5048938.
   PubMed Web of Science ®Google Scholar
• Gard AL, Luu RJ, Maloney R, et al. A high-throughput, 28-day, microfluidic model of gingival tissue inflammation and recovery. Commun Biol. 2023;6(1):92. doi: 10.1038/s42003-023-04434-9.
   PubMed Web of Science ®Google Scholar
• Danku AE, Dulf E-H, Braicu C, et al. Organ-on-a-chip: a survey of technical results and problems [review]. Front Bioeng Biotechnol. 2022;10:840674. doi: 10.3389/fbioe.2022.840674.
   PubMed Web of Science ®Google Scholar