The antioxidant response mechanism of flavonoids in ‘Tainong 1’ mango pulp under enhanced UV-B radiation

Figures & data

Table 1. List of UV-B radiation intensities in natural light (10–11 a.m.).

	First year (2021–2022)	Second year (2022–2023)
First sampling	617 kJ·m^−2·d^−1	609 kJ·m^−2·d^−1
Second sampling	608 kJ·m^−2·d^−1	598 kJ·m^−2·d^−1
Third sampling	589 kJ·m^−2·d^−1	621 kJ·m^−2·d^−1
Fourth sampling	611 kJ·m^−2·d^−1	622 kJ·m^−2·d^−1
Fifth sampling	602 kJ·m^−2·d^−1	588 kJ·m^−2·d^−1
Sixth sampling	628 kJ·m^−2·d^−1	631 kJ·m^−2·d^−1
Seventh sampling	607 kJ·m^−2·d^−1

Table 2. Primers used for the qRT–PCR assay.

Primer name	Left primer sequence (5′ to 3′)	Right primer sequence (5′ to 3′)
Mi00g09240.1	GGTGGAGGTCCCGGTTTC	GCACAGGGCGTAGAAGCA
Mi20g06400.1	GGGTTCAGGCTCTGGCAG	TCTTTTCGCGGTCGACGG
Mi09g04940.1	TGCGGCTCTTTGGGTCAC	TGGCGCAACACAATCCCA
Mi04g19560.1	ACATTCTCCAGCCCGCAC	ATCCGCCAAGTCCAAGCC
Mi04g07590.1	AGGGAGTCAGCGAGGAGG	TCAGCCTCAGATTCGCCG
Mi20g02530.1	GCCTGATGGAACGCCTGT	AGAACTGTCCGGCATGGC
Mi08g07240.1	AACGGAGGGGCATTGGTG	GTGTGATGCCCATCTGCCT
Mi05g08960.1	TGGAGGGGTGGTGCTCTT	CACCCCCTTTGGTTCCACA
Mi08g01120.1	GAGGTTGGCTGAGGCTGG	GGCCCTTGGTGTCAAGCA
Mi07g00970.1	TGTTGGCAACCGGGAGTG	CCTCTGCACAGTCGGCAA
Mi10g10300.1	AGGAGTCCTGGTGGGTGG	GCGTTTTGGGGCCCATTG
actin	ATCTGCTGGAAGGTGCTGAC	CCAAGCAGCATGAAGATCAA

Figure 1. Effect of enhanced UV-B radiation on dynamic changes in MDA content (a, b) and relative conductivity value of pulp (c, d).

Note: * indicates a significant difference at the level of p < 0.05. CK (control check) indicates the natural light control, T (UV-B treatment) indicates enhanced UV-B radiation treatment, and the same applies below.

Figure 2. Effect of enhanced UV-B radiation on dynamic changes in superoxide anion (a, b) and H₂O₂ content (c, d).

Figure 3. Effect of enhanced UV-B radiation on dynamic changes in total flavonoid (a, b) contents in pulp.

Figure 4. Bar plot of differentially expressed genes at 60 and 90 days after flowering (a) and KEGG enrichment analysis (b).

Figure 5. Correlation analysis between real-time PCR and transcriptome expression.

Table 3. Statistical table of the number of different metabolites (the former compared to the latter).

Group name	Total	Downregulated	Upregulated
CK-60DAF_vs_CK-90DAF	121	57	64
CK-60DAF_vs_T-60DAF	71	41	30
CK-90DAF_vs_T-90DAF	62	29	33
CK-30DAF_vs_CK-60DAF	190	106	84
CK-30DAF_vs_CK-90DAF	233	132	101
CK-30DAF_vs_T-60DAF	194	115	79
CK-30DAF_vs_T-90DAF	238	139	99
T-60DAF_vs_T-90DAF	149	58	91

Figure 7. Integrative analysis of the metabolome and transcriptome of flavonoids and flavonoid compounds in mango pulp of the treatment and control groups.

Note: CS1, CS2, CS3 and TS1, TS2, TS3 represent natural light control and enhanced UV-B radiation treatment at 30, 60 and 90 days after flowering.

Data availability statement

All data generated or analysed during this study are included in this published article.