Magnesium: does it reduce ischemia/reperfusion injury in an adnexal torsion rat model?

Figures & data

Figure 1 Torsion model performed in ovaries.

Table 1 Distribution of histopathologic evaluation scores in all groups

Group	Edema	Hemorrhage	Inflammatory cell
Group 1	0.50±0.83	0.33±0.51	0.83±0.98
Group 2	1.16±1.16	0.83±0.75	1.00±01.26
Group 3	2.83±0.40a,b	2.66±0.51a,b	2.80±0.44a,b
Group 4	0.66±0.81	0.50±0.83	0.50±0.83
Group 5	1.00±1.09	0.50+0.54	0.66±0.81
Group 6	1.16±0.75	2.16±0.75a	0.83±0.40c

Notes: Group 1, sham; Group 2, bilateral ovarian torsion; Group 3, bilateral ovarian torsion–detorsion; Group 4, MgSO₄–sham; Group 5, MgSO₄–bilateral ovarian torsion; Group 6, bilateral ovarian torsion–MgSO₄–detorsion. Data are expressed as the mean ± standard deviation.

a p<0.05 when compared to Group 1;

b p<0.05 when compared to Group 2;

c p<0.05 when compared to Group 3. Data presented as mean ± SD.

Abbreviation: MgSO₄, magnesium sulfate.

Figure 2 Histopathologic examination of the tissues (hematoxylin and eosin).

Notes: (A) sham group, (B) torsion group, (C) torsion–detorsion group, (D) MgSO₄–sham group, (E) MgSO₄–torsion group, (F) torsion–MgSO₄–detorsion group. (A, D) normal ovarian tissue structures in the sham and sham–MgSO₄ groups, (B, E) increase in edema (black star) and hemorrhage (black arrow) in the torsion and MgSO₄–torsion groups. (C) More prominent edema (black stars), hemorrhage (black arrows) and inflammatory cell infiltration (green arrows) in the torsion–detorsion group, (F) decrease in inflammatory cells with persistence of edema (black star) and hemorrhage (black arrows) in the MgSO₄ applied torsion–detorsion group.
Abbreviation: MgSO₄, magnesium sulfate.

Table 2 Ovarian tissue TOS, TAS, OSI, MDA and GSH levels and SOD, CAT and GSH-Px activities of all groups

Markers	Group 1	Group 2	Group 3	Group 4	Group 5	Group 6
TOS (µmol H2O2 Eq/g protein)	7.01±0.48	10.10±1.68*,a	12.71±3.95***,b	7.78±1.00	6.39±0.65**,c	6.36±0.30***,d
TAS (mmol Trolax Eq/g protein)	0.50±0.09	0.45±0.03	0.35±0.12*,b	0.52±0.04	0.42±0.07	0.37±0.02
OSI (arbitrary unit)	1.44±0.36	2.22±0.39	3.90±1.67***,b	1.50±0.27	1.54±0.26	1.71±0.14***,d
MDA (nmol/g protein)	6.29±0.79	8.33±0.86*,a	10.17±1.10***,b	4.73±1.15	5.88±0.40**,c	6.37±1.92***,d
SOD (U/g protein)	41.02±12.93	42.99±9.48	35.93±6.61	43.49±10.19	45.02±13.35	49.63±12.29
CAT (k/g)	4.04±0.87	3.04±0.69	2.41±0.44**,b	4.12±0.97	3.62±0.85	3.14±0.80
GSH-Px (U/g protein)	43.03±7.67	32.25±4.38	29.94±5.50*,b	48.79±11.90	44.39±5.77*,c	42.55±7.64*,d
GSH (nmol/mg protein)	3.14±0.52	2.03±0.30***,a	1.84±0.15***,b	3.23±0.54	2.77±0.34*,c	2.58±0.47*,d

Notes: Group 1, sham: Group 2, bilateral ovarian torsion; Group 3, bilateral ovarian torsion–detorsion; Group 4, MgSO₄–sham; Group 5, MgSO₄–bilateral ovarian torsion; Group 6, bilateral ovarian torsion–MgSO₄–detorsion. Data are expressed as the mean ± standard deviation.

* p<0.05;

** p<0.01;

*** p<0.001.

a Group 2 vs Group 1;

b Group 3 vs Group 1;

c Group 5 vs Group 2;

d Group 6 vs Group 3.

Abbreviations: TOS, total oxidant status; TAS, total antioxidant status; OSI, Oxidative Stress Index; MDA, malondialdehyde; GSH, glutathione; SOD, superoxide dismutase; CAT, catalase; GSH-Px, glutathione peroxidase; MgSO₄, magnesium sulfate.