Enhancing lymphatic uptake of Darunavir Ethanolate through TPGS-enriched lipid nanocarriers for oral delivery

References

• Aashish, A. Muheem, N. Nehal, A. Sartaj, S. Baboota, and J. Ali. 2023. Importance of p-gp inhibitors and nanoengineered approaches for effective delivery of anti-retroviral drugs across barriers in HIV management. Journal of Drug Delivery Science and Technology 87: 104791. doi: 10.1016/j.jddst.2023.104791.
   Web of Science ®Google Scholar
• AboulFotouh, K., A. A. Allam, M. El-Badry, and A. M. El-Sayed. 2019. A self-nanoemulsifying drug delivery system for enhancing the oral bioavailability of Candesartan cilexetil: Ex vivo and in vivo evaluation. Journal of Pharmaceutical Sciences 108(11): 3599–608. doi: 10.1016/j.xphs.2019.07.004.
   PubMed Web of Science ®Google Scholar
• Ahammed, V., R. Narayan, J. Paul, Y. Nayak, B. Roy, G. V. Shavi, and U. Y. Nayak. 2017. Development and in vivo evaluation of functionalized ritonavir proliposomes for lymphatic targeting. Life Sciences 183: 11–20. doi: 10.1016/j.lfs.2017.06.022.
   PubMed Web of Science ®Google Scholar
• Aji Alex, M. R., A. J. Chacko, S. Jose, and E. B. Souto. 2011. Lopinavir loaded solid lipid nanoparticles (SLN) for intestinal lymphatic targeting. Journal of the European Federation for Pharmaceutical Sciences 42 (1-2): 11–8. doi: 10.1016/j.ejps.2010.10.002. 20971188
   PubMed Web of Science ®Google Scholar
• Alam, T., S. Khan, B. Gaba, M. F. Haider, S. Baboota, and J. Ali. 2018. Adaptation of quality by design-based development of isradipine nanostructured-lipid carrier and its evaluation for in vitro gut permeation and in vivo solubilization fate. Journal of Pharmaceutical Sciences 107(11): 2914–26. doi: 10.1016/j.xphs.2018.07.021.
   PubMed Web of Science ®Google Scholar
• Alex, M. R., A. J. Chacko, S. Jose, and E. B. Souto. 2011. Lopinavir loaded solid lipid nanoparticles (SLN) for intestinal lymphatic targeting. European Journal of Pharmaceutical Sciences 42(1–2): 11–8. doi: 10.1016/j.ejps.2010.10.002.
   PubMed Web of Science ®Google Scholar
• Alihosseini, F., S. Ghaffari, A. R. Dabirsiaghi, and S. Haghighat. 2015. Freeze-drying of ampicillin solid lipid nanoparticles using mannitol as cryoprotectant. Brazilian Journal of Pharmaceutical Sciences 51(4): 797–802. doi: 10.1590/S1984-82502015000400005.
   Web of Science ®Google Scholar
• Annu, S. B., and J. Ali. 2022. In vitro appraisals and ex vivo permeation prospect of chitosan nanoparticles designed for schizophrenia to intensify nasal delivery. Polymer Bulletin, 79(4): 2263–85. doi: 10.1007/s00289-021-03598-w.
   Web of Science ®Google Scholar
• Araujo, V. H. S., P. B. da Silva, I. O. Szlachetka, S. W. da Silva, B. Fonseca-Santos, M. Chorilli, R. Ganassin, G. R. T. de Oliveira, M. C. O. da Rocha, R. P. Fernandes, et al. 2020. The influence of NLC composition on curcumin loading under a physicochemical perspective and in vitro evaluation. Colloids and Surfaces A: Physicochemical and Engineering Aspects 602: 125070. doi: 10.1016/j.colsurfa.2020.125070.
   Web of Science ®Google Scholar
• Attari, Z., A. Bhandari, P. C. Jagadish, and S. Lewis. 2016. Enhanced ex vivo intestinal absorption of Olmesartan medoxomil nanosuspension: Preparation by combinative technology. Saudi Pharmaceutical Journal 24(1): 57–63. doi: 10.1016/j.jsps.2015.03.008.
   PubMed Web of Science ®Google Scholar
• Bagasra, O. 2006. A unified concept of HIV latency. Expert Opinion on Biological Therapy 6(11): 1135–49. doi: 10.1517/14712598.6.11.1135.
   PubMed Web of Science ®Google Scholar
• Bonde, S., C. G. Bonde, and B. Prabhakar. 2019. Quality by design based development and validation of HPLC method for simultaneous estimation of paclitaxel and vinorelbine tartrate in dual drug loaded liposomes. Microchemical Journal 149: 103982. doi: 10.1016/j.microc.2019.103982.
   Web of Science ®Google Scholar
• Cirri, M., M. Bragagni, N. Mennini, and P. Mura. 2012. Development of a new delivery system consisting in ‘“drug – in cyclodextrin – in nanostructured lipid carriers”’ for ketoprofen topical delivery. European Journal of Pharmaceutics and Biopharmaceutics 80(1): 46–53. doi: 10.1016/j.ejpb.2011.07.015.
   PubMed Web of Science ®Google Scholar
• Cruz Silva, M. M., V. M. C. Madeira, L. M. Almeida, and J. B. A. Custódio. 2000. Hemolysis of human erythrocytes induced by tamoxifen is related to disruption of membrane structure. Biochimica et Biophysica Acta 1464(1): 49–61. doi: 10.1016/s0005-2736(99)00237-0.
   PubMed Web of Science ®Google Scholar
• Economidou, S. N., D. A. Lamprou, and D. Douroumis. 2018. 3D printing applications for transdermal drug delivery. International Journal of Pharmaceutics 544(2): 415–24. doi: 10.1016/j.ijpharm.2018.01.031.
   PubMed Web of Science ®Google Scholar
• Elgart, A., I. Cherniakov, Y. Aldouby, A. J. Domb, and A. Hoffman. 2013. Improved oral bioavailability of BCS class 2 compounds by self nano-emulsifying drug delivery systems (SNEDDS): The underlying mechanisms for amiodarone and talinolol. Pharmaceutical Research 30(12): 3029–44. doi: 10.1007/s11095-013-1063-y.
   PubMed Web of Science ®Google Scholar
• Elkateb, H., H. Cauldbeck, E. Niezabitowska, C. Hogarth, K. Arnold, S. Rannard, and T. O. McDonald. 2023. High drug loading solid lipid nanoparticles, nanostructured lipid carriers and nanoemulsions for the dual drug delivery of the HIV drugs darunavir and ritonavir. JCIS Open 11: 100087. doi: 10.1016/j.jciso.2023.100087.
   Google Scholar
• Elkateb, H., L. M. Tatham, H. Cauldbeck, E. Niezabitowska, A. Owen, S. Rannard, and T. McDonald. 2020. Optimization of the synthetic parameters of lipid polymer hybrid nanoparticles dual loaded with darunavir and ritonavir for the treatment of HIV. International Journal of Pharmaceutics 588: 119794. doi: 10.1016/j.ijpharm.2020.119794.
   PubMed Web of Science ®Google Scholar
• Emami, J., H. Yousefian, and H. Sadeghi. 2018. Targeted nanostructured lipid carrier for brain delivery of artemisinin: Design, preparation, characterization, optimization and cell toxicity. Journal of Pharmacy & Pharmaceutical Sciences 21(1s): 225s–41s. doi: 10.18433/jpps30117.
   PubMedGoogle Scholar
• Ganesan, P, and D. Narayanasamy. 2017. Lipid nanoparticles: Different preparation techniques, characterization, hurdles, and strategies for the production of solid lipid nanoparticles and nanostructured lipid carriers for oral drug delivery. Sustainable Chemistry and Pharmacy 6:37–56. 10.1016/j.scp.2017.07.002.
   Web of Science ®Google Scholar
• Garg, B., S. Beg, R. Kaur, R. Kumar, O. P. Katare, and B. Singh. 2018. Long-chain triglycerides-based self-nanoemulsifying oily formulations (SNEOFs) of darunavir with improved lymphatic targeting potential. Journal of Drug Targeting 26(3): 252–66. doi: 10.1080/1061186X.2017.1365875.
   PubMed Web of Science ®Google Scholar
• Garg, N. K., G. Sharma, B. Singh, P. Nirbhavane, R. K. Tyagi, R. Shukla, and O. P. Katare. 2017. Quality by design (QbD)-enabled development of aceclofenac loaded-nano structured lipid carriers (NLCs): An improved dermatokinetic profile for inflammatory disorder(s). International Journal of Pharmaceutics 517(1–2): 413–31. doi: 10.1016/j.ijpharm.2016.12.010.
   PubMed Web of Science ®Google Scholar
• González-Mira, E., S. Nikolić, M. L. García, M. A. Egea, E. B. Souto, and A. C. Calpena. 2011. Potential use of nanostructured lipid carriers for topical delivery of flurbiprofen. Journal of Pharmaceutical Sciences 100(1): 242–51. doi: 10.1002/jps.22271.
   PubMed Web of Science ®Google Scholar
• Gupta, B. K., S. Kumar, H. Kaur, J. Ali, and S. Baboota. 2018. Attenuation of oxidative damage by coenzyme Q10 loaded nanoemulsion through oral route for the management of Parkinson’s disease. Rejuvenation Research 21(3): 232–48. doi: 10.1089/rej.2017.1959.
   PubMed Web of Science ®Google Scholar
• Gurumukhi, V. C, and S. B. Bari. 2022. Quality by design (QbD)–based fabrication of atazanavir-loaded nanostructured lipid carriers for lymph targeting: bioavailability enhancement using chylomicron flow block model and toxicity studies. Drug Delivery and Translational Research 12 (5):1230–52. 10.1007/s13346-021-01014-4.
   PubMed Web of Science ®Google Scholar
• Halder, J., D. Pradhan, B. Kar, G. Ghosh, and G. Rath. 2022. Nanotherapeutics approaches to overcome P-glycoprotein-mediated multi-drug resistance in cancer. Nanomedicine: Nanotechnology, Biology, and Medicine 40: 102494. doi: 10.1016/j.nano.2021.102494.
   PubMed Web of Science ®Google Scholar
• Hobson, J. J., S. Edwards, R. A. Slater, P. Martin, A. Owen, and S. P. Rannard. 2018. Branched copolymer-stabilised nanoemulsions as new candidate oral drug delivery systems. RSC Advances 8(23): 12984–91. doi: 10.1039/c8ra01944d.
   PubMed Web of Science ®Google Scholar
• Innes, A., A. M. Farrell, R. P. Burden, A. G. Morgan, and R. J. Powell. 1994. Complement activation by cellulosic dialysis membranes. Journal of Clinical Pathology 47(2): 155–8. doi: 10.1136/jcp.47.2.155.
   PubMed Web of Science ®Google Scholar
• Inugala, S., B. B. Eedara, S. Sunkavalli, R. Dhurke, P. Kandadi, R. Jukanti, and S. Bandari. 2015. Solid self-nanoemulsifying drug delivery system (S-SNEDDS) of darunavir for improved dissolution and oral bioavailability: In vitro and in vivo evaluation. European Journal of Pharmaceutical Sciences 74: 1–10. doi: 10.1016/j.ejps.2015.03.024.
   PubMed Web of Science ®Google Scholar
• Iqubal, M. K., A. Kamal, A. Iqubal, M. Imran, J. Ali, and S. Baboota. 2021. Development and validation of a robust HPLC method for simultaneous estimation of 5-fluorouracil and resveratrol and its application in the engineered nanostructured lipid carrier. Current Analytical Chemistry 17(3): 385–95. doi: 10.2174/1573411016999200619184403.
   Web of Science ®Google Scholar
• Izham, M. N. M., Y. Hussin, N. F. C. Rahim, M. N. M. Aziz, S. K. Yeap, H. S. Rahman, M. J. Masarudin, N. E. Mohamad, R. Abdullah, and N. B. Alitheen. 2021. Physicochemical characterization, cytotoxic effect and toxicity evaluation of nanostructured lipid carrier loaded with eucalyptol. BMC Complementary Medicine and Therapies 21(1): 254. doi: 10.1186/s12906-021-03422-y.
   PubMedGoogle Scholar
• Jaiswal, P., B. Gidwani, and A. Vyas. 2016. Nanostructured lipid carriers and their current application in targeted drug delivery. Artificial Cells, Nanomedicine, and Biotechnology 44(1): 27–40. doi: 10.3109/21691401.2014.909822.
   PubMed Web of Science ®Google Scholar
• Jindal, A. B., S. S. Bachhav, and P. V. Devarajan. 2017. In situ hybrid nano drug delivery system (IHN-DDS) of the antiretroviral drug for simultaneous targeting multiple viral reservoirs: An in vivo proof of concept. International Journal of Pharmaceutics 521(1–2): 196–203. doi: 10.1016/j.ijpharm.2017.02.024.
   PubMed Web of Science ®Google Scholar
• Joshi, A. S., H. S. Patel, V. S. Belgamwar, A. Agrawal, and A. R. Tekade. 2012. Solid lipid nanoparticles of ondansetron HCl for intranasal delivery: Development, optimization and evaluation. Journal of Materials Science: Materials in Medicine 23(9): 2163–75. doi: 10.1007/s10856-012-4702-7.
   PubMed Web of Science ®Google Scholar
• Khan, S., M. Ganguli, A. Aditya, S. Khan, S. Baboota, and J. Ali. 2019. Improved in vivo performance and immunomodulatory effect of novel Omega-3 fatty acid-based Tacrolimus nanostructured lipid carrier. Journal of Drug Delivery Science and Technology 52: 138–49. doi: 10.1016/j.jddst.2019.04.019.
   Web of Science ®Google Scholar
• Khan, S., M. Shaharyar, M. Fazil, M. Q. Hassan, S. Baboota, and J. Ali. 2016. Tacrolimus loaded nanostructured lipid carriers for oral delivery-In vivo bioavailability enhancement. European Journal of Pharmaceutics and Biopharmaceutics 109: 149–57. doi: 10.1016/j.ejpb.2016.10.011.
   PubMed Web of Science ®Google Scholar
• Khan, S. A., S. Rehman, B. Nabi, A. Iqubal, N. Nehal, U. A. Fahmy, S. Kotta, S. Baboota, M. Shadab, and J. Ali. 2020. Boosting the brain delivery of atazanavir through nanostructured lipid carrier-based approach for mitigating neuroaids. Pharmaceutics 12(11): 1059. doi: 10.3390/pharmaceutics12111059.
   PubMed Web of Science ®Google Scholar
• Kincl, M., S. Turk, and F. Vrecer. 2005. Application of experimental design methodology in development and optimization of drug release method. International Journal of Pharmaceutics 291(1–2): 39–49. doi: 10.1016/j.ijpharm.2004.07.041.
   PubMed Web of Science ®Google Scholar
• Luiz, M. T., L. D. D. Filippo, R. C. Alves, V. H. S. Araújo, J. L. Duarte, J. M. Marchetti, and M. Chorilli. 2021. The use of TPGS in drug delivery systems to overcome biological barriers. European Polymer Journal 142: 110129. doi: 10.1016/j.eurpolymj.2020.110129.
   Web of Science ®Google Scholar
• Luiz, M. T., J. S. R. Viegas, J. P. Abriata, F. Viegas, F. T. M. C. Vicentini, M. V. L. B. Bentley, M. Chorilli, J. M. Marchetti, and D. R. Tapia-Blácido. 2021. Design of experiments (DoE) to develop and to optimize nanoparticles as drug delivery systems. European Journal of Pharmaceutics and Biopharmaceutics 165: 127–48. doi: 10.1016/j.ejpb.2021.05.011.
   PubMed Web of Science ®Google Scholar
• Luo, T., C. Wu, and L. Duan. 2018. Fishbone diagram and risk matrix analysis method and its application in safety assessment of natural gas spherical tank. Journal of Cleaner Production 174: 296–304. doi: 10.1016/j.jclepro.2017.10.334.
   Web of Science ®Google Scholar
• Malamatari, M., K. M. G. Taylor, S. Malamataris, D. Douroumis, and K. Kachrimanis. 2018. Pharmaceutical nanocrystals: Production by wet milling and applications. Drug Discovery Today 23(3): 534–47. doi: 10.1016/j.drudis.2018.01.016.
   PubMed Web of Science ®Google Scholar
• McKeage, K., C. M. Perry, and S. J. Keam. 2009. Darunavir: A review of its use in the management of HIV infection in adults. Drugs 69(4): 477–503. doi: 10.2165/00003495-200969040-00007.
   PubMed Web of Science ®Google Scholar
• Mishra, N., S. Sharma, R. Deshmukh, A. Kumar, and R. Sharma. 2019. Development and characterization of nasal delivery of selegiline hydrochloride loaded nanolipid carriers for the management of Parkinson’s disease. Central Nervous System Agents in Medicinal Chemistry 19(1): 46–56. doi: 10.2174/1871524919666181126124846.
   PubMedGoogle Scholar
• Muheem, A., S. Baboota, and J. Ali. 2021. An in-depth analysis of novel combinatorial drug therapy via nanocarriers against HIV/AIDS infection and their clinical perspectives: a systematic review. Expert Opinion on Drug Delivery 18 (8):1025–46. doi: 10.1080/17425247.2021.1876660.
   PubMed Web of Science ®Google Scholar
• Muheem, A., M. Wasim, E. Aldosari, S. Baboota, and J. Ali. 2023. Fabrication of TPGS decorated Etravirine loaded lipidic nanocarriers as a neoteric oral bioavailability enhancer for lymphatic targeting. Discov Nano 19 (1):1–5. doi: 10.1186/s11671-023-03954-x.
   Google Scholar
• Nabi, B., S. Rehman, S. Aggarwal, S. Baboota, and J. Ali. 2020. Quality by design adapted chemically engineered lipid architectonics for HIV therapeutics and intervention: Contriving of formulation, appraising the in vitro parameters and in vivo solubilization potential. AAPS PharmSciTech 21(7): 261. doi: 10.1208/s12249-020-01795-w.
   PubMed Web of Science ®Google Scholar
• Negi, L. M., M. Jaggi, and S. Talegaonkar. 2014. Development of protocol for screening the formulation components and assessing common quality problems of nanostructured lipid carriers. International Journal of Pharmaceutics 461(1–2): 403–10. doi: 10.1016/j.ijpharm.2013.12.006.
   PubMed Web of Science ®Google Scholar
• Neupane, Y. R., M. Srivastava, N. Ahmad, N. Kumar, A. Bhatnagar, and K. Kohli. 2014. Lipid based nanocarrier system for the potential oral delivery of decitabine: Formulation design, characterization, ex vivo, and in vivo assessment. International Journal of Pharmaceutics 477(1–2): 601–12. doi: 10.1016/j.ijpharm.2014.11.001.
   PubMed Web of Science ®Google Scholar
• Pai, R. V, and P.R. Vavia. 2020. Chitosan oligosaccharide enhances binding of nanostructured lipid carriers to ocular mucins: Effect on ocular disposition. International Journal of Pharmaceutics 577:119095 10.1016/j.ijpharm.2020.119095.
   PubMed Web of Science ®Google Scholar
• Peczek, S. H., A. P. S. Tartari, I. C. Zittlau, C. Diedrich, C. S. Machado, and R. M. Mainardes. 2023. Enhancing oral bioavailability and brain biodistribution of perillyl alcohol using nanostructured lipid carriers. Pharmaceuticals 16(8): 1055. doi: 10.3390/ph16081055.
   Web of Science ®Google Scholar
• Pham, C. V., and C. Cho. 2017. Application of d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) in transdermal and topical drug delivery systems (TDDS). Journal of Pharmaceutical Investigation 47(2): 111–21. doi: 10.1007/s40005-016-0300-x.
   Google Scholar
• Pokharkar, V., A. Patil-Gadhe, and G. Kaur. 2018. Physicochemical and pharmacokinetic evaluation of rosuvastatin loaded nanostructured lipid carriers: Influence of long- and medium-chain fatty acid mixture. Journal of Pharmaceutical Investigation 48(4): 465–76. doi: 10.1007/s40005-017-0342-8.
   Google Scholar
• Qamar, Z., M. U. Ashhar, Annu, F. F. Qizilibash, P. K. Sahoo, A. Ali, J. Ali, and S. Baboota. 2021. Lipid nanocarrier of selegiline augmented anti-Parkinson’s effect via p-gp modulation using quercetin. International Journal of Pharmaceutics 609: 121131. doi: 10.1016/j.ijpharm.2021.121131.
   PubMed Web of Science ®Google Scholar
• Reddy, B. P., P. Khadgapathi, and G. K. Reddy. 2018. Darunavir compositions. EP Patent 2,568,810, filed May 10, 2010, and issued September 19, 2018.
   Google Scholar
• Rehman, S., B. Nabi, S. Baboota, and J. Ali. 2021. Tailoring lipid nanoconstructs for the oral delivery of paliperidone: Formulation, optimization and in vitro evaluation. Chemistry and Physics of Lipids 234: 105005. doi: 10.1016/j.chemphyslip.2020.105005.
   PubMed Web of Science ®Google Scholar
• Rojekar, S., L. Fotooh Abadi, R. Pai, K. Mahajan, S. Kulkarni, and P. R. Vavia. 2021. Multi-organ targeting of HIV-1 viral reservoirs with etravirine loaded nanostructured lipid carrier: An in-vivo proof of concept. European Journal of Pharmaceutical Sciences 164:105916 10.1016/j.ejps.2021.105916.
   PubMed Web of Science ®Google Scholar
• Ronaldson, P. T., Y. Persidsky, and R. Bendayan. 2008. Regulation of ABC membrane transporters in glial cells: Relevance to the pharmacotherapy of brain HIV-1 infection. Glia 56(16): 1711–35. doi: 10.1002/glia.20725.
   PubMed Web of Science ®Google Scholar
• Salome, A. C., O. C. Godswill, and O. I. Ikechukwu. 2013. Kinetics and mechanisms of drug release from swellable and non swellable matrices: A review. Research Journal of Pharmaceutical, Biological and Chemical Sciences 4(2): 94–103.
   Google Scholar
• Sartaj, A., M. Alam, L. Biswas, M. S. Yar, S. R. Mir, A. K. Verma, S. Baboota, and J. Ali. 2022. Combinatorial delivery of ribociclib and green tea extract mediated nanostructured lipid carrier for oral delivery for the treatment of breast cancer synchronising in silico, in vitro, and in vivo studies. Journal of Drug Targeting 30(10): 1113–34. doi: 10.1080/1061186X.2022.2104292.
   PubMed Web of Science ®Google Scholar
• Shete, H., S. Chatterjee, A. De, and V. Patravale. 2013. Long chain lipid based tamoxifen NLC. Part II: Pharmacokinetic, biodistribution and in vitro anticancer efficacy studies. International Journal of Pharmaceutics 454(1): 584–92. doi: 10.1016/j.ijpharm.2013.03.036.
   PubMed Web of Science ®Google Scholar
• Shevalkar, G, and P. Vavia. 2019. Solidified nanostructured lipid carrier (S-NLC) for enhancing the oral bioavailability of ezetimibe. Journal of Drug Delivery Science and Technology 53:101211. doi: 10.1016/j.jddst.2019.101211.
   Web of Science ®Google Scholar
• Singh, H., S. Jindal, M. Singh, G. Sharma, and I. P. Kaur. 2015. Nanoformulation of rifampicin with enhanced bioavailability: Development, characterization and in-vivo safety. International Journal of Pharmaceutics 485(1–2): 138–51. doi: 10.1016/j.ijpharm.2015.02.050.
   PubMed Web of Science ®Google Scholar
• Tan, S., C. Zou, W. Zhang, M. Yin, X. Gao, and Q. Tang. 2017. Recent developments in d-α-tocopheryl polyethylene glycol-succinate-based nanomedicine for cancer therapy. Drug Delivery 24(1): 1831–42. doi: 10.1080/10717544.2017.1406561.
   PubMed Web of Science ®Google Scholar
• Tarsitano, M., M. C. Cristiano, A. Mancuso, A. Barone, D. Torella, and D. Paolino. 2022. Lipid-based formulations containing labrafil M2125-CS: A deep investigation on nanosystem stability. Nanomanufacturing 2(1): 41–52. doi: 10.3390/nanomanufacturing2010003.
   Google Scholar
• Tsai, M. J., P. C. Wu, Y. B. Huang, J. S. Chang, C. L. Lin, Y. H. Tsai, and J. Y. Fang. 2012. Baicalein loaded in tocol nanostructured lipid carriers (tocol NLCs) for enhanced stability and brain targeting. International Journal of Pharmaceutics 423(2): 461–70. doi: 10.1016/j.ijpharm.2011.12.009.
   PubMed Web of Science ®Google Scholar
• UNAIDS. 2023. Global AIDS Update. Assessed on July 25, 2023. https://thepath.unaids.orgwp-content/themes/unaids2023/assets/files/2023_report.pdf
   Google Scholar
• World Health Organization (WHO). 2023. HIV/AIDS/Fact Sheets-2023. https://www.who.int/news-room/fact-sheets/detail/hiv-aids. Assessed July 25, 2023.
   Google Scholar
• Yallapu, M. M., M. C. Ebeling, N. Chauhan, M. Jaggi, and S. C. Chauhan. 2011. Interaction of curcumin nanoformulations with human plasma proteins and erythrocytes. International Journal of Nanomedicine 6: 2779–90. doi: 10.2147/IJN.S25534.
   PubMed Web of Science ®Google Scholar