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Improving the integrity and reproducibility of research that uses antibodies: a technical, data sharing, behavioral and policy challenge

M. Biddlea NIHR Respiratory BRC, Department of Respiratory Sciences, University of Leicester, Leicester, UKORCID Iconhttps://orcid.org/0000-0002-9853-8815View further author information
ORCID Icon,
P. Stylianoua NIHR Respiratory BRC, Department of Respiratory Sciences, University of Leicester, Leicester, UKORCID Iconhttps://orcid.org/0000-0002-0904-6043View further author information
ORCID Icon,
M. Rekasa NIHR Respiratory BRC, Department of Respiratory Sciences, University of Leicester, Leicester, UKView further author information
,
A. Wrighta NIHR Respiratory BRC, Department of Respiratory Sciences, University of Leicester, Leicester, UKORCID Iconhttps://orcid.org/0000-0003-1621-1622View further author information
ORCID Icon,
J. Sousaa NIHR Respiratory BRC, Department of Respiratory Sciences, University of Leicester, Leicester, UKView further author information
,
D. Ruddya NIHR Respiratory BRC, Department of Respiratory Sciences, University of Leicester, Leicester, UKView further author information
,
M. I. Stefanab JDRF/Wellcome Diabetes and Inflammation Laboratory, Wellcome Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford, UKORCID Iconhttps://orcid.org/0000-0002-4796-5884View further author information
ORCID Icon,
K. Kmiecika NIHR Respiratory BRC, Department of Respiratory Sciences, University of Leicester, Leicester, UKView further author information
,
A. Bandrowskic Department of Neuroscience, UC San Diego, La Jolla, CA, USAORCID Iconhttps://orcid.org/0000-0002-5497-0243View further author information
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R.A. Kahnd Department of Biochemistry, Emory University School of Medicine, Atlanta, USAORCID Iconhttps://orcid.org/0000-0002-0259-0601View further author information
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C. Laflammee Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, CanadaORCID Iconhttps://orcid.org/0000-0001-5906-025XView further author information
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E. M. Krockowf School of Psychology and Vision Sciences, University of Leicester, Leicester, UKORCID Iconhttps://orcid.org/0000-0001-8748-9897View further author information
ORCID Icon &
H.S. Virka NIHR Respiratory BRC, Department of Respiratory Sciences, University of Leicester, Leicester, UKCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0002-9739-9593View further author information
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Article: 2323706 | Received 21 Jan 2024, Accepted 22 Feb 2024, Published online: 06 Mar 2024

References

  • Goodman SL. The antibody horror show: an introductory guide for the perplexed. N Biotechnol. 2018;45:9–8. doi:10.1016/j.nbt.2018.01.006.
  • Baker M. Antibody anarchy: a call to order. Nature. 2015;527(7579):545–51. doi:10.1038/527545a.
  • Baker M. Reproducibility crisis: blame it on the antibodies. Nature. 2015;521(7552):274. doi:10.1038/521274a.
  • Björling E, Uhlén M. Antibodypedia, a portal for sharing antibody and antigen validation data. Mol Cell Proteomics. 2008;7(10):2028–37. doi:10.1074/mcp.M800264-MCP200.
  • Uhlén M, Fagerberg L, Hallström BM, Lindskog C, Oksvold P, Mardinoglu A, Sivertsson Å, Kampf C, Sjöstedt E, Asplund A. et al. Proteomics. Tissue-based map of the human proteome. Sci (New York, NY). 2015;347(6220):1260419–. doi:10.1126/science.1260419.
  • Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M. et al. A proposal for validation of antibodies. Nat Methods. 2016;13(10):823–7. doi:10.1038/nmeth.3995.
  • Andersson S, Sundberg M, Pristovsek N, Ibrahim A, Jonsson P, Katona B, Clausson C-M, Zieba A, Ramström M, Söderberg O. et al. Insufficient antibody validation challenges oestrogen receptor beta research. Nat Commun. 2017;8(1):1–14. doi:10.1038/ncomms15840.
  • Bradbury A, Plückthun A. Reproducibility: standardize antibodies used in research. Nature. 2015;518(7537):27–29. doi:10.1038/518027a.
  • Virk H, Rekas M, Biddle M, Wright A, Sousa J, Weston C, Chachi L, Roach KM, Bradding P. Validation of antibodies for the specific detection of human TRPA1. Sci Rep. 2019;9(1):1–9. doi:10.1038/s41598-019-55133-7.
  • Aponte Santiago N, Konecki S, Porter L, Virk H. Tales of the unexpected. Elife. 2023;12:e87444. doi:10.7554/eLife.87444.
  • Freedman LP, Cockburn IM, Simcoe TS. The economics of reproducibility in preclinical research. PLoS Biol. 2015;13(6):e1002165. doi:10.1371/journal.pbio.1002165.
  • Voskuil JL, Bandrowski A, Begley CG, Bradbury AR, Chalmers ad, Gomes AV, Hardcastle T, Lund-Johansen F, Pluckthun A, Roncador G, et al. The antibody Society’s antibody validation webinar series. MAbs (Vol. 12, No. 1, p. 1794421); 2020: Taylor & Francis.
  • Maier T, Güell M, Serrano L. Correlation of mRNA and protein in complex biological samples. FEBS Lett. 2009;583(24):3966–73. doi:10.1016/j.febslet.2009.10.036.
  • Ayoubi R, Ryan J, Biddle MS, Alshafie W, Fotouhi M, Bolivar SG, Ruiz Moleon V, Eckmann P, Worrall D, McDowell I. et al. Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications. Elife. 2023;12:RP91645. doi:10.7554/eLife.91645.
  • Freedman LP, Gibson MC, Bradbury AR, Buchberg AM, Davis D, Dolled-Filhart MP, Lund-Johansen F, Rimm DL. The need for improved education and training in research antibody usage and validation practices. Biotechniques. 2016;61(1):16–18. doi:10.2144/000114431.
  • Michie S, Van Stralen MM, West R. The behaviour change wheel: a new method for characterising and designing behaviour change interventions. Implement Sci. 2011;6(1):1–12. doi:10.1186/1748-5908-6-42.
  • Berglund L, Björling E, Oksvold P, Fagerberg L, Asplund A, Szigyarto C-K, Persson A, Ottosson J, Wernérus H, Nilsson P. et al. A genecentric human protein atlas for expression profiles based on antibodies. Mol Cell Proteomics. 2008;7(10):2019–27. doi:10.1074/mcp.R800013-MCP200.
  • Kužílková D, Puñet-Ortiz J, Aui PM, Fernández J, Fišer K, Engel P, van Zelm MC, Kalina T. Standardization of workflow and flow cytometry panels for quantitative expression profiling of surface antigens on blood leukocyte subsets: an HCDM CDMaps initiative. Front Immunol. 2022;13:827898. doi:10.3389/fimmu.2022.827898.
  • Laflamme C, Edwards AM, Bandrowski AE, McPherson PS. Opinion: Independent third-party entities as a model for validation of commercial antibodies. N Biotechnol. 2021;65:1–8. doi:10.1016/j.nbt.2021.07.001.
  • Biddle MS, Virk HS. YCharOS open antibody characterisation data: lessons learned and progress made. F1000 Research Limited London, UK. 2023;12:1344. doi:10.12688/f1000research.141719.1.
  • Longworth S, Chalmers A, Duine M. CiteAb for researchers and suppliers: how identifying product citations from publications can help accelerate science. Inf Serv Use. 2022;42(3–4):319–24. doi:10.3233/ISU-220158.
  • Pauly D, Hanack K. How to avoid pitfalls in antibody use. F1000Research. 2015;4:4. doi:10.12688/f1000research.6894.1.
  • Vasilevsky NA, Brush MH, Paddock H, Ponting L, Tripathy SJ, LaRocca GM, Haendel MA. On the reproducibility of science: unique identification of research resources in the biomedical literature. PeerJ. 2013;1:e148. doi:10.7717/peerj.148.
  • Bandrowski AE, Martone ME. Rrids: a simple step toward improving reproducibility through rigor and transparency of experimental methods. Neuron. 2016;90(3):434–6. doi:10.1016/j.neuron.2016.04.030.
  • Xie H. Validated antibody database (VAD): a database of antibodies curated from publications. FASEB J. 2017;31(S1):.983.3–.3. doi:10.1096/fasebj.31.1_supplement.983.3.
  • Helsby MA, Leader PM, Fenn JR, Gulsen T, Bryant C, Doughton G, Sharpe B, Whitley P, Caunt CJ, James K. et al. CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited. BMC Cell Biol. 2014;15(1):1–12. doi:10.1186/1471-2121-15-6.
  • Laflamme C, McKeever PM, Kumar R, Schwartz J, Kolahdouzan M, Chen CX, You Z, Benaliouad F, Gileadi O, McBride HM. et al. Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. Elife. 2019;8. doi:10.7554/eLife.48363.
  • O’Doherty N, Marketing F. Software powered by artificial intelligence accelerates drug discovery. https://www.nature.com/articles/d43747-022-00125-2.

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