mAbs, Volume 16, Issue 1 (2024)

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mAbs, Volume 16, Issue 1 (2024)

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Volume 16, 2024 Vol 15, 2023 Vol 14, 2022 Vol 13, 2021 Vol 12, 2020 Vol 11, 2019 Vol 10, 2018 Vol 9, 2017 Vol 8, 2016 Vol 7, 2015 Vol 6, 2014 Vol 5, 2013 Vol 4, 2012 Vol 3, 2011 Vol 2, 2010 Vol 1, 2009
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Predicting the clinical subcutaneous absorption rate constant of monoclonal antibodies using only the primary sequence: a machine learning approach
Ronghua Bei, Justin Thomas, Shiven Kapur, Mahlet Woldeyes, Adam Rauk, Jason Robarge, Jiangyan Feng & Kaoutar Abbou Oucherif
Article: 2352887
Published online: 14 May 2024
AbstractforPredicting the clinical subcutaneous absorption rate constant of monoclonal antibodies using only the primary sequence: a machine learning approach | Full Text | References | PDF (4.5 MB) | EPUB | Supplemental
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Correction

Correction
Correction
Article: 2354626
Published online: 12 May 2024
CitationforCorrection | Full Text | PDF (585.2 KB) | EPUB
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Antibody association in solution: cluster distributions and mechanisms
Sandi Brudar, Leonid Breydo, Elisha Chung, Ken A. Dill, Nasim Ehterami, Ketan Phadnis, Samir Senapati, Mohammed Shameem, Xiaolin Tang, Muhammmad Tayyab & Barbara Hribar-Lee
Article: 2339582
Published online: 26 Apr 2024
AbstractforAntibody association in solution: cluster distributions and mechanisms | Full Text | References | PDF (2.9 MB) | EPUB | Supplemental
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Review

Review Article
Applications and challenges in designing VHH-based bispecific antibodies: leveraging machine learning solutions
Michael Mullin, James McClory, Winston Haynes, Justin Grace, Nathan Robertson & Gino van Heeke
Article: 2341443
Published online: 26 Apr 2024
AbstractforApplications and challenges in designing VHH-based bispecific antibodies: leveraging machine learning solutions | Full Text | References | PDF (3.3 MB) | EPUB
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Targeted CQA analytical control strategy for commercial antibody products: Replacing ion-exchange chromatography methods for charge heterogeneity with multi-attribute monitoring
Adam R. Evans, Joseph Mulholland, Michael J. Lewis & Ping Hu
Article: 2341641
Published online: 23 Apr 2024
AbstractforTargeted CQA analytical control strategy for commercial antibody products: Replacing ion-exchange chromatography methods for charge heterogeneity with multi-attribute monitoring | Full Text | References | PDF (948.3 KB) | EPUB | Supplemental
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Alternative splicing for tuneable expression of protein subunits at desired ratios
Christel Aebischer-Gumy, Pierre Moretti, Timothee Brunstein Laplace, Jana Frank, Ysaline Grand, Farid Mosbaoui, Emilie Hily, Anna Galea, Megane Peltret, Carole Estoppey, Daniel Ayoub, Roberto Giovannini & Martin Bertschinger
Article: 2342243
Published online: 22 Apr 2024
AbstractforAlternative splicing for tuneable expression of protein subunits at desired ratios | Full Text | References | PDF (2.7 MB) | EPUB | Supplemental
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Reducing neonatal Fc receptor binding enhances clearance and brain-to-blood ratio of TfR-delivered bispecific amyloid-β antibody
Eva Schlein, Ken G. Andersson, Tiffany Dallas, Stina Syvänen & Dag Sehlin
Article: 2339337
Published online: 18 Apr 2024
AbstractforReducing neonatal Fc receptor binding enhances clearance and brain-to-blood ratio of TfR-delivered bispecific amyloid-β antibody | Full Text | References | PDF (3.1 MB) | EPUB
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Review

Review Article
Can antibodies be “vegan”? A guide through the maze of today’s antibody generation methods
Stefan Dübel
Article: 2343499
Published online: 18 Apr 2024
AbstractforCan antibodies be “vegan”? A guide through the maze of today’s antibody generation methods | Full Text | References | PDF (2 MB) | EPUB
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Enrichment strategy and initial characterization of heterodimers enriched from a co-formulated cocktail of therapeutic antibodies against SARS-COV-2
Sophia Liu, Yuetian Yan, Cody M. Secor, Zachary R. Oberholtzer, Donna J. Skow, Mushhood Sheikh, Youmi Moon, Yue Fu, Cristinel Sandu, Shunhai Wang, Ning Li, Jennifer B. Nguyen, Michael P. Rosconi & Erica A. Pyles
Article: 2338301
Published online: 09 Apr 2024
AbstractforEnrichment strategy and initial characterization of heterodimers enriched from a co-formulated cocktail of therapeutic antibodies against SARS-COV-2 | Full Text | References | PDF (2.4 MB) | EPUB | Supplemental
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Predicting deamidation and isomerization sites in therapeutic antibodies using structure-based in silico approaches
David Hoffmann, Joschka Bauer, Markus Kossner, Andrew Henry, Anne R. Karow-Zwick & Giuseppe Licari
Article: 2333436
Published online: 28 Mar 2024
AbstractforPredicting deamidation and isomerization sites in therapeutic antibodies using structure-based in silico approaches | Full Text | References | PDF (2.4 MB) | EPUB | Supplemental
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Correction

Correction
Correction
Article: 2335597
Published online: 28 Mar 2024
CitationforCorrection | Full Text | PDF (3.5 MB) | EPUB
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Commentary and Views

Comment
In silico methods for immunogenicity risk assessment and human homology screening for therapeutic antibodies
Aimee E. Mattei, Andres H. Gutierrez, Soorya Seshadri, Jacob Tivin, Matt Ardito, Amy S. Rosenberg, William D. Martin & Anne S. De Groot
Article: 2333729
Published online: 27 Mar 2024
AbstractforIn silico methods for immunogenicity risk assessment and human homology screening for therapeutic antibodies | Full Text | References | PDF (3.8 MB) | EPUB | Supplemental
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Evaluation of the impact of antibody fragments on aggregation of intact molecules via size exclusion chromatography coupled with native mass spectrometry
Jing Xu, John E. Coughlin, Malgorzata Szyjka, Serene Jabary, Sonal Saluja, Zoran Sosic, Yunqiu Chen & Chong-Feng Xu
Article: 2334783
Published online: 27 Mar 2024
AbstractforEvaluation of the impact of antibody fragments on aggregation of intact molecules via size exclusion chromatography coupled with native mass spectrometry | Full Text | References | PDF (8 MB) | EPUB | Supplemental
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RUBY® – a tetravalent (2+2) bispecific antibody format with excellent functionality and IgG-like stability, pharmacology and developability properties
Barnabas Nyesiga, Mattias Levin, Anna Säll, Anna Rosén, Kim Jansson, Sara Fritzell, Karin Hägerbrand, Dietmar Weilguny & Laura von Schantz
Article: 2330113
Published online: 25 Mar 2024
AbstractforRUBY® – a tetravalent (2+2) bispecific antibody format with excellent functionality and IgG-like stability, pharmacology and developability properties | Full Text | References | PDF (1.9 MB) | EPUB | Supplemental
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Review

Review Article
T and B cell epitope analysis for the immunogenicity evaluation and mitigation of antibody-based therapeutics
Ruoxuan Sun, Mark G. Qian & Xiaobin Zhang
Article: 2324836
Published online: 21 Mar 2024
AbstractforT and B cell epitope analysis for the immunogenicity evaluation and mitigation of antibody-based therapeutics | Full Text | References | PDF (1.3 MB) | EPUB
  • 2844 Views
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Antibody-epitope conjugates deliver immunogenic T-cell epitopes more efficiently when close to cell surfaces
W. van der Wulp, W. Luu, M. E. Ressing, J. Schuurman, S. I. van Kasteren, L. Guelen, R. C. Hoeben, B. Bleijlevens & M. H. M. Heemskerk
Article: 2329321
Published online: 18 Mar 2024
AbstractforAntibody-epitope conjugates deliver immunogenic T-cell epitopes more efficiently when close to cell surfaces | Full Text | References | PDF (2.7 MB) | EPUB | Supplemental
  • 1228 Views
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Do antibody CDR loops change conformation upon binding?
Chu’nan Liu, Lilian M. Denzler, Oliver E.C. Hood & Andrew C.R. Martin
Article: 2322533
Published online: 13 Mar 2024
AbstractforDo antibody CDR loops change conformation upon binding? | Full Text | References | PDF (3.7 MB) | EPUB | Supplemental
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Reduction of monoclonal antibody viscosity using interpretable machine learning
Emily K. Makowski, Hsin-Ting Chen, Tiexin Wang, Lina Wu, Jie Huang, Marissa Mock, Patrick Underhill, Emma Pelegri-O’Day, Erick Maglalang, Dwight Winters & Peter M. Tessier
Article: 2303781
Published online: 12 Mar 2024
AbstractforReduction of monoclonal antibody viscosity using interpretable machine learning | Full Text | References | PDF (3.3 MB) | EPUB | Supplemental
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Perspectives

Comment
A pivotal decade for bispecific antibodies?
Marlena Surowka & Christian Klein
Article: 2321635
Published online: 11 Mar 2024
AbstractforA pivotal decade for bispecific antibodies? | Full Text | References | PDF (4.3 MB) | EPUB
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Exploring molecular determinants and pharmacokinetic properties of IgG1-scFv bispecific antibodies
Kristina M.J. Aertker, Minu Ravindra Pilvankar, Tobias M. Prass, Michaela Blech, Fabian Higel & Srinath Kasturirangan
Article: 2318817
Published online: 06 Mar 2024
AbstractforExploring molecular determinants and pharmacokinetic properties of IgG1-scFv bispecific antibodies | Full Text | References | PDF (2.9 MB) | EPUB | Supplemental
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A novel conditional active biologic anti-EpCAM x anti-CD3 bispecific antibody with synergistic tumor selectivity for cancer immunotherapy
Gerhard Frey, Ana Paula G. Cugnetti, Haizhen Liu, Charles Xing, Christina Wheeler, Hwai Wen Chang, William J. Boyle & Jay M. Short
Article: 2322562
Published online: 06 Mar 2024
AbstractforA novel conditional active biologic anti-EpCAM x anti-CD3 bispecific antibody with synergistic tumor selectivity for cancer immunotherapy | Full Text | References | PDF (4 MB) | EPUB | Supplemental
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Perspectives

Comment
Improving the integrity and reproducibility of research that uses antibodies: a technical, data sharing, behavioral and policy challenge
M. Biddle, P. Stylianou, M. Rekas, A. Wright, J. Sousa, D. Ruddy, M. I. Stefana, K. Kmiecik, A. Bandrowski, R.A. Kahn, C. Laflamme, E. M. Krockow & H.S. Virk
Article: 2323706
Published online: 06 Mar 2024
AbstractforImproving the integrity and reproducibility of research that uses antibodies: a technical, data sharing, behavioral and policy challenge | Full Text | References | PDF (1.1 MB) | EPUB
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Meeting Report

Meeting Report
Proceedings of the 14th European immunogenicity platform open symposium on immunogenicity of biopharmaceuticals
Sophie Tourdot, Daniel Baltrunkonis, Sofie Denies, Viswanath Devanarayan, Joanna Grudzinska-Goebel, Arno Kromminga, Gregor P. Lotz, Laurent Malherbe, Lydia Michaut, Karin N. Weldingh, Joao A. Pedras-Vasconcelos, Laura. I. Salazar-Fontana, Sebastian Spindeldreher, Zuben Sauna, Veerle Snoeck, Daniela Verthelyi & Daniel Kramer
Article: 2324801
Published online: 05 Mar 2024
AbstractforProceedings of the 14th European immunogenicity platform open symposium on immunogenicity of biopharmaceuticals | Full Text | References | PDF (290.5 KB) | EPUB
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Review

Review Article
Biparatopic antibodies: therapeutic applications and prospects
David L. Niquille, Kyle M. Fitzgerald & Nimish Gera
Article: 2310890
Published online: 04 Mar 2024
AbstractforBiparatopic antibodies: therapeutic applications and prospects | Full Text | References | PDF (1.8 MB) | EPUB
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Application of quantitative protein mass spectrometric data in the early predictive analysis of membrane-bound target engagement by monoclonal antibodies
Armin Sepp & Morris Muliaditan
Article: 2324485
Published online: 04 Mar 2024
AbstractforApplication of quantitative protein mass spectrometric data in the early predictive analysis of membrane-bound target engagement by monoclonal antibodies | Full Text | References | PDF (4.1 MB) | EPUB | Supplemental
  • 926 Views
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Review

Review Article
Facilitating high throughput bispecific antibody production and potential applications within biopharmaceutical discovery workflows
Caitlin Fawcett, Joseph. R. Tickle & Charlotte. H. Coles
Article: 2311992
Published online: 21 Feb 2024
AbstractforFacilitating high throughput bispecific antibody production and potential applications within biopharmaceutical discovery workflows | Full Text | References | PDF (2.2 MB) | EPUB
  • 3514 Views
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Production of antibodies and antibody fragments containing non-natural amino acids in Escherichia coli
Jacquelyn Blake-Hedges, Dan Groff, Wilson Foo, Jeffrey Hanson, Elenor Castillo, Miao Wen, Diana Cheung, Mary Rose Masikat, Jian Lu, Young Park, Nina Abi Carlos, Hans Usman, Kevin Fong, Abigail Yu, Sihong Zhou, Joyce Kwong, Cuong Tran, Xiaofan Li, Dawei Yuan, Trevor Hallam & Gang Yin
Article: 2316872
Published online: 21 Feb 2024
AbstractforProduction of antibodies and antibody fragments containing non-natural amino acids in Escherichia coli | Full Text | References | PDF (1.2 MB) | EPUB | Supplemental
  • 1634 Views
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Impact of antibody architecture and paratope valency on effector functions of bispecific NKp30 x EGFR natural killer cell engagers
Ammelie Svea Boje, Lukas Pekar, Katharina Koep, Britta Lipinski, Brian Rabinovich, Andreas Evers, Carina Lynn Gehlert, Steffen Krohn, Yanping Xiao, Simon Krah, Rinat Zaynagetdinov, Lars Toleikis, Sven Poetzsch, Matthias Peipp, Stefan Zielonka & Katja Klausz
Article: 2315640
Published online: 19 Feb 2024
AbstractforImpact of antibody architecture and paratope valency on effector functions of bispecific NKp30 x EGFR natural killer cell engagers | Full Text | References | PDF (2.6 MB) | EPUB | Supplemental
  • 2775 Views
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Structure- and machine learning-guided engineering demonstrate that a non-canonical disulfide in an anti-PD-1 rabbit antibody does not impede antibody developability
Wei-Ching Liang, Hongkang Xi, Dawei Sun, Luigi D’Ascenzo, Jonathan Zarzar, Nicole Stephens, Ryan Cook, Yinyin Li, Zhengmao Ye, Marissa Matsumoto, Jian Payandeh, Matthieu Masureel & Yan Wu
Article: 2309685
Published online: 14 Feb 2024
AbstractforStructure- and machine learning-guided engineering demonstrate that a non-canonical disulfide in an anti-PD-1 rabbit antibody does not impede antibody developability | Full Text | References | PDF (2.6 MB) | EPUB | Supplemental
  • 1891 Views
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Report
CC-96673 (BMS-986358), an affinity-tuned anti-CD47 and CD20 bispecific antibody with fully functional fc, selectively targets and depletes non-Hodgkin’s lymphoma
Dan Zhu, Haralambos Hadjivassiliou, Catherine Jennings, David Mikolon, Massimo Ammirante, Sharmistha Acharya, Jon Lloyd, Mahan Abbasian, Rama Krishna Narla, Joseph R. Piccotti, Katie Stamp, Ho Cho & Kandasamy Hariharan
Article: 2310248
Published online: 13 Feb 2024
AbstractforCC-96673 (BMS-986358), an affinity-tuned anti-CD47 and CD20 bispecific antibody with fully functional fc, selectively targets and depletes non-Hodgkin’s lymphoma | Full Text | References | PDF (3 MB) | EPUB | Supplemental
  • 1787 Views
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Electrostatic properties of human germlines and biodistribution of small biologics
Leticia Maria De Souza Cordeiro, Kelley Christine Atkinson, Argin Aivazian, Patrick Frank Joyce, Fang Jia & Alessandro Mascioni
Article: 2311991
Published online: 09 Feb 2024
AbstractforElectrostatic properties of human germlines and biodistribution of small biologics | Full Text | References | PDF (2.1 MB) | EPUB | Supplemental
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Correction

Correction
Correction
Article: 2312050
Published online: 08 Feb 2024
CitationforCorrection | Full Text | PDF (1.1 MB) | EPUB
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Perspectives

Comment
Charge heterogeneity of therapeutic monoclonal antibodies by different cIEF systems: views on the current situation
Alessandro Ascione, Marcello Belfiore, Jaana Vesterinen, Mihaela Buda, Wolf Holtkamp & Francesca Luciani
Article: 2313737
Published online: 08 Feb 2024
AbstractforCharge heterogeneity of therapeutic monoclonal antibodies by different cIEF systems: views on the current situation | Full Text | References | PDF (822.5 KB) | EPUB | Supplemental
  • 1320 Views
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Report

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In-situ biophysical characterization of high-concentration protein formulations using wNMR
Jing Song, Marc Taraban, Y. Bruce Yu, Lynn Lu, Pallavi Guha Biswas, Wei Xu, Hanmi Xi, Akhilesh Bhambhani, Guangli Hu & Yongchao Su
Article: 2304624
Published online: 01 Feb 2024
AbstractforIn-situ biophysical characterization of high-concentration protein formulations using wNMR | Full Text | References | PDF (2.1 MB) | EPUB | Supplemental
  • 1593 Views
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Variable domain mutational analysis to probe the molecular mechanisms of high viscosity of an IgG1 antibody
Jing Dai, Saeed Izadi, Jonathan Zarzar, Patrick Wu, Angela Oh & Paul J. Carter
Article: 2304282
Published online: 25 Jan 2024
AbstractforVariable domain mutational analysis to probe the molecular mechanisms of high viscosity of an IgG1 antibody | Full Text | References | PDF (3.3 MB) | EPUB | Supplemental
  • 3300 Views
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A tailored lectin microarray for rapid glycan profiling of therapeutic monoclonal antibodies
Shen Luo & Baolin Zhang
Article: 2304268
Published online: 22 Jan 2024
AbstractforA tailored lectin microarray for rapid glycan profiling of therapeutic monoclonal antibodies | Full Text | References | PDF (12.1 MB) | EPUB | Supplemental
  • 1118 Views
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Interactions of the anti-FcRn monoclonal antibody, rozanolixizumab, with Fcγ receptors and functional impact on immune cells in vitro
Omar S. Qureshi, Emma J. Sutton, Rosemary F. Bithell, Shauna M. West, Rona M. Cutler, Gillian McCluskey, Graham Craggs, Asher Maroof, Nicholas M. Barnes, David P. Humphreys, Stephen Rapecki, Bryan J. Smith & Anthony Shock
Article: 2300155
Published online: 19 Jan 2024
AbstractforInteractions of the anti-FcRn monoclonal antibody, rozanolixizumab, with Fcγ receptors and functional impact on immune cells in vitro | Full Text | References | PDF (2.1 MB) | EPUB | Supplemental
  • 2000 Views
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Engineering hydrophobicity and manufacturability for optimized biparatopic antibody–drug conjugates targeting c-MET
Andreas Evers, Simon Krah, Deniz Demir, Ramona Gaa, Desislava Elter, Christian Schroeter, Stefan Zielonka, Nicolas Rasche, Julia Dotterweich, Christine Knuehl & Achim Doerner
Article: 2302386
Published online: 12 Jan 2024
AbstractforEngineering hydrophobicity and manufacturability for optimized biparatopic antibody–drug conjugates targeting c-MET | Full Text | References | PDF (3.9 MB) | EPUB | Supplemental
  • 4469 Views
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Perspectives

Antibodies to watch in 2024
Silvia Crescioli, Hélène Kaplon, Alicia Chenoweth, Lin Wang, Jyothsna Visweswaraiah & Janice M. Reichert
Article: 2297450
Published online: 05 Jan 2024
AbstractforAntibodies to watch in 2024 | Full Text | References | PDF (932.7 KB) | EPUB | Supplemental
  • 17393 Views
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Therapeutic efficacy of a potent anti-Venezuelan equine encephalitis virus antibody is contingent on Fc effector function
Jennifer L. Schwedler, Maxwell A. Stefan, Christine E. Thatcher, Peter R. McIlroy, Anupama Sinha, Ashlee M. Phillips, Christopher A. Sumner, Colleen M. Courtney, Christina Y. Kim, Dina R. Weilhammer & Brooke Harmon
Article: 2297451
Published online: 03 Jan 2024
AbstractforTherapeutic efficacy of a potent anti-Venezuelan equine encephalitis virus antibody is contingent on Fc effector function | Full Text | References | PDF (3.8 MB) | EPUB | Supplemental
  • 1080 Views
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Comprehensive characterization of higher order structure changes in methionine oxidized monoclonal antibodies via NMR chemometric analysis and biophysical approaches
Mingyue Li, Victor A. Beaumont, Shahajahan Akbar, Hannah Duncan, Arch Creasy, Wenge Wang, Kelly Sackett, Lisa Marzilli, Jason C. Rouse & Hai-Young Kim
Article: 2292688
Published online: 20 Dec 2023
AbstractforComprehensive characterization of higher order structure changes in methionine oxidized monoclonal antibodies via NMR chemometric analysis and biophysical approaches | Full Text | References | PDF (2.7 MB) | EPUB | Supplemental
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