Pigs are highly susceptible to but do not transmit mink-derived highly pathogenic avian influenza virus H5N1 clade 2.3.4.4b

References

• Tong S, Li Y, Rivailler P, et al. A distinct lineage of influenza A virus from bats. Proc Natl Acad Sci U S A. 2012;109(11):4269–4274. DOI:10.1073/pnas.1116200109
   PubMed Web of Science ®Google Scholar
• Tong S, Zhu X, Li Y, et al. New world bats harbor diverse influenza A viruses. PLoS Pathog. 2013;9(10):e1003657. DOI:10.1371/journal.ppat.1003657
   PubMed Web of Science ®Google Scholar
• Vey M, Orlich M, Adler S, et al. Hemagglutinin activation of pathogenic avian influenza viruses of serotype H7 requires the protease recognition motif R-X-K/R-R. Virology. 1992;188(1):408–413. DOI:10.1016/0042-6822(92)90775-K
   PubMed Web of Science ®Google Scholar
• Xu X, Subbarao, Cox NJ, et al. Genetic characterization of the pathogenic influenza A/Goose/Guangdong/1/96 (H5N1) virus: similarity of its hemagglutinin gene to those of H5N1 viruses from the 1997 outbreaks in Hong Kong. Virology. 1999;261(1):15–19. DOI:10.1006/viro.1999.9820
   PubMed Web of Science ®Google Scholar
• WHO/OIE/FAO H5N1 Evolution Working Group. Toward a unified nomenclature system for highly pathogenic avian influenza virus (H5N1). Emerg Infect Dis. 2008;14(7):e1. DOI:10.3201/eid1407.071681
   PubMed Web of Science ®Google Scholar
• World Health Organization. Evolution of the influenza A(H5) haemagglutinin: WHO/OIE/FAO H5 Working Group reports a new clade designated 2.3.4.4 2015. Available from: https://www.who.int/publications/m/item/evolution-of-the-influenza-a(h5)-haemagglutinin-who-oie-fao-h5-working-group-reports-a-new-clade-designated-2.3.4.4.
   Google Scholar
• Pohlmann A, King J, Fusaro A, et al. Has epizootic become enzootic? Evidence for a fundamental change in the infection dynamics of highly pathogenic avian influenza in Europe, 2021. mBio. 2022;13(4):e0060922.
   PubMed Web of Science ®Google Scholar
• Bevins SN, Shriner SA, Cumbee JC, et al. Intercontinental movement of highly pathogenic avian influenza A(H5N1) clade 2.3.4.4 virus to the United States, 2021. Emerg Infect Dis. 2022;28(5):1006–1011. DOI:10.3201/eid2805.220318
   PubMed Web of Science ®Google Scholar
• Shinya K, Ebina M, Yamada S, et al. Influenza virus receptors in the human airway. Nature. 2006;440(7083):435–436. DOI:10.1038/440435a
   PubMed Web of Science ®Google Scholar
• World Health Organization. Avian influenza weekly update number. 2023;904.
   Google Scholar
• Harfoot R, Webby RJ. H5 influenza, a global update. J Microbiol. 2017;55(3):196–203. DOI:10.1007/s12275-017-7062-7
   PubMed Web of Science ®Google Scholar
• World Health Organization. Assessment of risk associated with recent influenza A (H5N1) clade 2.3.4.4b viruses. 2022.
   Google Scholar
• Aguero M, Monne I, Sanchez A, et al. Highly pathogenic avian influenza A(H5N1) virus infection in farmed minks, Spain, October 2022. Euro Surveill. 2023;28:3. DOI:10.2807/1560-7917.ES.2023.28.3.2300001
   Google Scholar
• Ma W, Belisle SE, Mosier D, et al. 2009 pandemic H1N1 influenza virus causes disease and upregulation of genes related to inflammatory and immune responses, cell death, and lipid metabolism in pigs. J Virol. 2011;85(22):11626–11637. DOI:10.1128/JVI.05705-11
   PubMed Web of Science ®Google Scholar
• Reed LJ, Muench H. A simple method of estimating fifty per cent endpoints12. Am J Epidemiol 1938;27(3):493–497. DOI:10.1093/oxfordjournals.aje.a118408
   Google Scholar
• Gaudreault NN, Trujillo JD, Carossino M, et al. SARS-CoV-2 infection, disease and transmission in domestic cats. Emerg Microbes Infect. 2020;9(1):2322–2332. DOI:10.1080/22221751.2020.1833687
   PubMed Web of Science ®Google Scholar
• Sponseller BA, Strait E, Jergens A, et al. Influenza A pandemic (H1N1) 2009 virus infection in domestic cat. Emerg Infect Dis. 2010;16(3):534–537. DOI:10.3201/eid1603.091737
   PubMed Web of Science ®Google Scholar
• Gauger PC, Vincent AL. Serum virus neutralization assay for detection and quantitation of serum neutralizing antibodies to influenza A virus in swine. Methods Mol Biol. 2020;2123:321–333. DOI:10.1007/978-1-0716-0346-8_23
   PubMedGoogle Scholar
• Artiaga BL, Morozov I, Ransburgh R, et al. Evaluating alpha-galactosylceramide as an adjuvant for live attenuated influenza vaccines in pigs. Anim Dis. 2022;2(1):19. DOI:10.1186/s44149-022-00051-x
   PubMedGoogle Scholar
• Lee J, Henningson J, Ma J, et al. Effects of PB1-F2 on the pathogenicity of H1N1 swine influenza virus in mice and pigs. J Gen Virol. 2017;98(1):31–42. DOI:10.1099/jgv.0.000695
   PubMed Web of Science ®Google Scholar
• Lee DH. Complete genome sequencing of influenza A viruses using next-generation sequencing. Methods Mol Biol. 2020;2123:69–79. DOI:10.1007/978-1-0716-0346-8_6
   PubMedGoogle Scholar
• Zhou B, Wentworth DE. Influenza A virus molecular virology techniques. Methods Mol Biol. 2012;865:175–192. DOI:10.1007/978-1-61779-621-0_11
   PubMedGoogle Scholar
• Hoffmann E, Stech J, Guan Y, et al. Universal primer set for the full-length amplification of all influenza A viruses. Arch Virol. 2001;146(12):2275–2289. DOI:10.1007/s007050170002
   PubMed Web of Science ®Google Scholar
• Mena I, Nelson MI, Quezada-Monroy F, et al. Origins of the 2009 H1N1 influenza pandemic in swine in Mexico. Elife. 2016;28:5.
   Google Scholar
• Joseph U, Su YC, Vijaykrishna D, et al. The ecology and adaptive evolution of influenza A interspecies transmission. Influenza Other Respir Viruses. 2017;11(1):74–84. DOI:10.1111/irv.12412
   PubMed Web of Science ®Google Scholar
• Ma W, Vincent AL, Gramer MR, et al. Identification of H2N3 influenza A viruses from swine in the United States. Proc Natl Acad Sci U S A. 2007;104(52):20949–20954. DOI:10.1073/pnas.0710286104
   PubMed Web of Science ®Google Scholar
• Garten RJ, Davis CT, Russell CA, et al. Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans. Science. 2009;325(5937):197–201. DOI:10.1126/science.1176225
   PubMed Web of Science ®Google Scholar
• World Health Organization. Tool for influenza pandemic risk assessment (TIPRA). 2020.
   Google Scholar
• Rajao DS, Vincent AL. Swine as a model for influenza A virus infection and immunity. ILAR J. 2015;56(1):44–52. DOI:10.1093/ilar/ilv002
   PubMed Web of Science ®Google Scholar
• Ito T, Couceiro JN, Kelm S, et al. Molecular basis for the generation in pigs of influenza A viruses with pandemic potential. J Virol. 1998;72(9):7367–7373. DOI:10.1128/JVI.72.9.7367-7373.1998
   PubMed Web of Science ®Google Scholar
• Kaplan BS, Torchetti MK, Lager KM, et al. Absence of clinical disease and contact transmission of HPAI H5NX clade 2.3.4.4 from North America in experimentally infected pigs. Influenza Other Respir Viruses. 2017;11(5):464–470. DOI:10.1111/irv.12463
   PubMed Web of Science ®Google Scholar
• Graaf A, Piesche R, Sehl-Ewert J, et al. Low susceptibility of pigs against experimental infection with HPAI virus H5N1 clade 2.3.4.4b. Emerg Infect Dis. 2023;29(7):1492–1495. DOI:10.3201/eid2907.230296
   PubMed Web of Science ®Google Scholar
• Elsmo EJ, Wunschmann A, Beckmen KB, et al. Highly pathogenic avian influenza A(H5N1) virus clade 2.3.4.4b infections in wild terrestrial mammals, United States, 2022. Emerg Infect Dis. 2023;29(12):2451–2460. DOI:10.3201/eid2912.230464
   PubMed Web of Science ®Google Scholar
• Thorsson E, Zohari S, Roos A, et al. Highly pathogenic avian influenza A(H5N1) virus in a harbor porpoise, Sweden. Emerg Infect Dis. 2023;29(4):852–855. DOI:10.3201/eid2904.221426
   PubMed Web of Science ®Google Scholar
• Bordes L, Vreman S, Heutink R, et al. Highly pathogenic avian influenza H5N1 virus infections in wild Red foxes (Vulpes vulpes) show neurotropism and adaptive virus mutations. Microbiol Spectr. 2023;11(1):e0286722. DOI:10.1128/spectrum.02867-22
   PubMed Web of Science ®Google Scholar
• Balzli C, Lager K, Vincent A, et al. Susceptibility of swine to H5 and H7 low pathogenic avian influenza viruses. Influenza Other Respir Viruses. 2016;10(4):346–352. DOI:10.1111/irv.12386
   PubMed Web of Science ®Google Scholar
• Richt JA, Lager KM, Janke BH, et al. Pathogenic and antigenic properties of phylogenetically distinct reassortant H3N2 swine influenza viruses cocirculating in the United States. J Clin Microbiol. 2003;41(7):3198–3205. DOI:10.1128/JCM.41.7.3198-3205.2003
   PubMed Web of Science ®Google Scholar
• Kida H, Ito T, Yasuda J, et al. Potential for transmission of avian influenza viruses to pigs. J Gen Virol. 1994;75(Pt 9):2183–2188. DOI:10.1099/0022-1317-75-9-2183
   PubMedGoogle Scholar
• De Vleeschauwer A, Van Poucke S, Braeckmans D, et al. Efficient transmission of swine-adapted but not wholly avian influenza viruses among pigs and from pigs to ferrets. J Infect Dis. 2009;200(12):1884–1892. DOI:10.1086/648475
   PubMed Web of Science ®Google Scholar
• Shortridge KF, Zhou NN, Guan Y, et al. Characterization of avian H5N1 influenza viruses from poultry in Hong Kong. Virology. 1998;252(2):331–342. DOI:10.1006/viro.1998.9488
   PubMed Web of Science ®Google Scholar
• Choi YK, Nguyen TD, Ozaki H, et al. Studies of H5N1 influenza virus infection of pigs by using viruses isolated in Vietnam and Thailand in 2004. J Virol. 2005;79(16):10821–5. DOI:10.1128/JVI.79.16.10821-10825.2005
   PubMed Web of Science ®Google Scholar
• Maemura T, Guan L, Gu C, et al. Characterization of highly pathogenic clade 2.3.4.4b H5N1 mink influenza viruses. EBioMedicine. 2023;97:104827. DOI:10.1016/j.ebiom.2023.104827
   PubMed Web of Science ®Google Scholar
• Lee JH, Pascua PN, Song MS, et al. Isolation and genetic characterization of H5N2 influenza viruses from pigs in Korea. J Virol. 2009;83(9):4205–4215. DOI:10.1128/JVI.02403-08
   PubMed Web of Science ®Google Scholar
• Bussey KA, Bousse TL, Desmet EA, et al. PB2 residue 271 plays a key role in enhanced polymerase activity of influenza A viruses in mammalian host cells. J Virol. 2010;84(9):4395–4406. DOI:10.1128/JVI.02642-09
   PubMed Web of Science ®Google Scholar
• Neumann G, Chen H, Gao GF, et al. H5N1 influenza viruses: outbreaks and biological properties. Cell Res. 2010;20(1):51–61. DOI:10.1038/cr.2009.124
   PubMed Web of Science ®Google Scholar
• Kim JH, Hatta M, Watanabe S, et al. Role of host-specific amino acids in the pathogenicity of avian H5N1 influenza viruses in mice. J Gen Virol. 2010;91(5):1284–1289. DOI:10.1099/vir.0.018143-0
   PubMedGoogle Scholar
• Herfst S, Schrauwen EJ, Linster M, et al. Airborne transmission of influenza A/H5N1 virus between ferrets. Science. 2012;336(6088):1534–1541. DOI:10.1126/science.1213362
   PubMed Web of Science ®Google Scholar
• Linster M, van Boheemen S, de Graaf M, et al. Identification, characterization, and natural selection of mutations driving airborne transmission of A/H5N1 virus. Cell. 2014;157(2):329–339. DOI:10.1016/j.cell.2014.02.040
   PubMed Web of Science ®Google Scholar
• Hatta M, Gao P, Halfmann P, et al. Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses. Science. 2001;293(5536):1840–1842. DOI:10.1126/science.1062882
   PubMed Web of Science ®Google Scholar
• Steel J, Lowen AC, Mubareka S, et al. Transmission of influenza virus in a mammalian host is increased by PB2 amino acids 627 K or 627E/701N. PLoS Pathog. 2009;5(1):e1000252. DOI:10.1371/journal.ppat.1000252
   PubMed Web of Science ®Google Scholar
• Taft AS, Ozawa M, Fitch A, et al. Identification of mammalian-adapting mutations in the polymerase complex of an avian H5N1 influenza virus. Nat Commun. 2015;6:7491. DOI:10.1038/ncomms8491
   PubMed Web of Science ®Google Scholar
• Song W, Wang P, Mok BW, et al. The K526R substitution in viral protein PB2 enhances the effects of E627 K on influenza virus replication. Nat Commun. 2014;5:5509. DOI:10.1038/ncomms6509
   PubMed Web of Science ®Google Scholar
• Stevens J, Blixt O, Tumpey TM, et al. Structure and receptor specificity of the hemagglutinin from an H5N1 influenza virus. Science. 2006;312(5772):404–410. DOI:10.1126/science.1124513
   PubMed Web of Science ®Google Scholar
• Lindh E, Lounela H, Ikonen N, et al. Highly pathogenic avian influenza A(H5N1) virus infection on multiple fur farms in the South and Central Ostrobothnia regions of Finland, 2023. Euro Surveill. 2023;28:31. DOI:10.2807/1560-7917.ES.2023.28.31.2300400
   Google Scholar
• Adlhoch C, Fusaro A, Gonzales JL, et al. Avian influenza overview April–June 2023. EFSA J. 2023;21(7):e08191.
   PubMed Web of Science ®Google Scholar